Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,311,049	T→C	8.0%	V267A (GTA→GCA) ‡	PP_2033 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,311,049	0	T	C	8.0%	176.3 / 4.6	62	V267A (GTA→GCA) ‡	PP_2033	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base C (2/3);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310903‑2311197                                                                                                                                                    |                                                                                                                                                     ggACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAg                                                                                                                                                    <  6:204779/149‑1 (MQ=255)     gAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAc                                                                                                                                                <  5:259529/149‑1 (MQ=255)          gcggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcact                                                                                                                                           <  7:97517/149‑4 (MQ=255)                    ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAcgc                                                                                                                                              >  5:63608/1‑136 (MQ=255)                    ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCAcgc                                                                                                                                              <  6:63608/136‑1 (MQ=255)                    ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcac                                                                                                                                            <  1:138497/138‑3 (MQ=255)                    ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcac                                                                                                                                            >  2:138497/1‑136 (MQ=255)                    ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGCGCGTGCCTACAGGCAcgca                                                                                                                                             <  4:105679/137‑2 (MQ=255)                    ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGCGCGTGCCTACAGGCAcgca                                                                                                                                             >  3:105679/1‑136 (MQ=255)                     tCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGc                                                                                                                                >  3:149860/1‑149 (MQ=255)                                       gCCACGACATCGTGCTGCCGCGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                               >  7:221571/1‑148 (MQ=255)                                         cACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAg                                                                                                            >  4:282965/1‑149 (MQ=255)                                         cACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAg                                                                                                            >  5:326822/1‑149 (MQ=255)                                          aCGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGt                                                                                                           <  1:129562/149‑1 (MQ=255)                                            gACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTTGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGc                                                                                                         <  4:57379/149‑1 (MQ=255)                                              cATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCt                                                                                                       <  7:42582/149‑1 (MQ=255)                                               aTCGTGCTGCCGGGCGTTCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGTGCAGGTGTTTCTGTGCAAGCCGCAGTCCCAGTGCGTTCCTGTAGGAACGCGTGTCGATCAGTGCCGCGTACATGCCAAGGTGAGTGCCtt                                                                                                      <  5:282787/149‑1 (MQ=255)                                                   tgctgcCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTg                                                                                                  <  5:41840/149‑1 (MQ=255)                                                     gtgcCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGc                                                                                                <  1:271320/148‑1 (MQ=255)                                                               tGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTTCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                                      <  4:149860/149‑1 (MQ=255)                                                                gCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTg                                                                                     >  5:114732/1‑149 (MQ=255)                                                                  aCTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGcc                                                                                   >  3:145854/1‑149 (MQ=255)                                                                    tCGAATATTGGGGGTGGTTACCCGGAGACCCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGcc                                                                                   >  1:193381/1‑148 (MQ=255)                                                                    tCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                                      >  4:93621/1‑144 (MQ=255)                                                                    tCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                                      <  3:93621/144‑1 (MQ=255)                                                                    tCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  4:91163/1‑149 (MQ=255)                                                                       aaTATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGatat                                                                              >  8:17918/1‑149 (MQ=255)                                                                       aaTATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTGTGCCGatat                                                                              >  8:13308/1‑149 (MQ=255)                                                                                    gtTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAgg                                                                 <  1:262550/149‑1 (MQ=255)                                                                                     ttACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGc                                                                >  3:102401/1‑149 (MQ=255)                                                                                        cccGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcg                                                              <  2:193381/148‑1 (MQ=255)                                                                                         ccGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                            >  6:279412/1‑149 (MQ=255)                                                                                             aga‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtg                                                        <  6:114732/149‑1 (MQ=255)                                                                                              ga‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtga                                                       >  2:204610/1‑149 (MQ=255)                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAg                                                                  >  1:221833/1‑135 (MQ=255)                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAg                                                                  <  2:221833/135‑1 (MQ=255)                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  7:159851/1‑149 (MQ=255)                                                                                                    ttCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAg                                                                  <  7:238008/133‑1 (MQ=255)                                                                                                    ttCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAg                                                                  >  8:238008/1‑133 (MQ=255)                                                                                                     tCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCt                                                 >  4:297313/1‑149 (MQ=255)                                                                                                        ggAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTgg                                               >  3:11008/1‑148 (MQ=255)                                                                                                              ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                        >  2:170276/1‑149 (MQ=255)                                                                                                                     cTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   >  7:19499/1‑147 (MQ=255)                                                                                                                     cTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   <  8:19499/147‑1 (MQ=255)                                                                                                                        tgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGcc                                <  4:145854/147‑1 (MQ=255)                                                                                                                        tgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                               <  6:326822/148‑1 (MQ=255)                                                                                                                           aaGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTgg                           >  2:300703/1‑149 (MQ=255)                                                                                                                           aaGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTgg                           <  1:300703/149‑1 (MQ=255)                                                                                                                             gCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggc                         <  8:159851/149‑1 (MQ=255)                                                                                                                               cGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcgg                       >  8:88538/1‑149 (MQ=255)                                                                                                                                gCAGTCCCAGTGCATGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGc                                 >  7:238654/1‑138 (MQ=255)                                                                                                                                gCAGTCCCAGTGCATGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGc                                 <  8:238654/138‑1 (MQ=255)                                                                                                                                 cAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                               >  6:82522/1‑139 (MQ=255)                                                                                                                                 cAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                               <  5:82522/139‑1 (MQ=255)                                                                                                                                 cAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGgcggcg                     <  3:282965/149‑1 (MQ=255)                                                                                                                                    tCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGa                  <  7:13308/149‑1 (MQ=255)                                                                                                                                     cccAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGa                  <  7:17918/148‑1 (MQ=255)                                                                                                                                          tgcgtgcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgc            <  4:102401/149‑1 (MQ=255)                                                                                                                                               gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                   >  3:44329/1‑121 (MQ=255)                                                                                                                                               gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                   >  3:48428/1‑121 (MQ=255)                                                                                                                                               gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                   <  4:48428/121‑1 (MQ=255)                                                                                                                                               gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                   <  4:44329/121‑1 (MQ=255)                                                                                                                                                    tAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGTCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGTGACGAGGCGCAAAAGCAg  <  8:221571/149‑1 (MQ=255)                                                                                                                                                    tAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGc    <  3:91163/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGA‑CCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310903‑2311197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑