Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,311,147 A→G 8.7% T300A (ACC→GCC)  PP_2033 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,311,1470AG 8.7% 124.4 / 3.1 46T300A (ACC→GCC) PP_2033hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base G (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

CCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTGGCGGTGCGCG  >  NC_002947/2310999‑2311285
                                                                                                                                                    |                                                                                                                                          
ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                                                                                                            >  7:159851/1‑149 (MQ=255)
   tCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCt                                                                                                                                         >  4:297313/1‑149 (MQ=255)
      ggAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTgg                                                                                                                                       >  3:11008/1‑148 (MQ=255)
            ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                                                                                                                >  2:170276/1‑149 (MQ=255)
                   cTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                                                                                                           <  8:19499/147‑1 (MQ=255)
                   cTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                                                                                                           >  7:19499/1‑147 (MQ=255)
                      tgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGcc                                                                                                                        <  4:145854/147‑1 (MQ=255)
                      tgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                                                                                                                       <  6:326822/148‑1 (MQ=255)
                         aaGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTgg                                                                                                                   <  1:300703/149‑1 (MQ=255)
                         aaGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTgg                                                                                                                   >  2:300703/1‑149 (MQ=255)
                           gCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggc                                                                                                                 <  8:159851/149‑1 (MQ=255)
                             cGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcgg                                                                                                               >  8:88538/1‑149 (MQ=255)
                              gCAGTCCCAGTGCATGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGc                                                                                                                         >  7:238654/1‑138 (MQ=255)
                              gCAGTCCCAGTGCATGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGc                                                                                                                         <  8:238654/138‑1 (MQ=255)
                               cAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                                                                                                                       <  5:82522/139‑1 (MQ=255)
                               cAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCg                                                                                                                       >  6:82522/1‑139 (MQ=255)
                               cAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGgcggcg                                                                                                             <  3:282965/149‑1 (MQ=255)
                                  tCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGa                                                                                                          <  7:13308/149‑1 (MQ=255)
                                   cccAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGa                                                                                                          <  7:17918/148‑1 (MQ=255)
                                        tgcgtgcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgc                                                                                                    <  4:102401/149‑1 (MQ=255)
                                             gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                                                                                                           >  3:44329/1‑121 (MQ=255)
                                             gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                                                                                                           <  4:48428/121‑1 (MQ=255)
                                             gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                                                                                                           <  4:44329/121‑1 (MQ=255)
                                             gcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGGCCTGGGAGGAGCCGAtt                                                                                                                           >  3:48428/1‑121 (MQ=255)
                                                  tAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGTCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGTGACGAGGCGCAAAAGCAg                                                                                          <  8:221571/149‑1 (MQ=255)
                                                  tAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGc                                                                                            <  3:91163/147‑1 (MQ=255)
                                                       aCGCGTGTCGAGCAGTGCCGCATACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTa                                                                                     >  6:44207/1‑149 (MQ=255)
                                                        cgcgTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTAt                                                                                    <  1:204610/149‑1 (MQ=255)
                                                            tgtCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATgcgg                                                                                >  5:161656/1‑149 (MQ=255)
                                                                  gCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGgtg                                                                           >  3:40448/1‑148 (MQ=255)
                                                                                  gCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGt                                                          >  3:115736/1‑149 (MQ=255)
                                                                                      aGGTGAGTGCCTTGCTGGCTTCGGCGTTTGGCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCg                                                      >  1:26297/1‑149 (MQ=255)
                                                                                         tGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCgggg                                                   <  5:279412/149‑1 (MQ=255)
                                                                                               ccTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACtt                                              <  3:297313/148‑1 (MQ=255)
                                                                                                       gCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTGCCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGgt                                     <  5:44207/149‑1 (MQ=255)
                                                                                                         ttCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCggg                                                                    <  6:117094/116‑1 (MQ=255)
                                                                                                         ttCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCggg                                                                    >  5:117094/1‑116 (MQ=255)
                                                                                                                               gAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGtt             <  4:11008/149‑1 (MQ=255)
                                                                                                                                 aCCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTgg           >  3:123485/1‑149 (MQ=255)
                                                                                                                                   ccAGGCCGCGCGTGTTGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTGGCg         >  1:146182/1‑149 (MQ=255)
                                                                                                                                   ccAGGCCGCGCGTGATGACCTGGGAGGAGCCAATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTgg           <  7:215612/147‑1 (MQ=255)
                                                                                                                                   ccAGGCCGCGCGTGATGACCTGGGAGGAGCCAATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTgg           >  8:215612/1‑147 (MQ=255)
                                                                                                                                        ccGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTGGCGGt       <  5:213040/146‑1 (MQ=255)
                                                                                                                                        ccGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTGGCGGt       >  6:213040/1‑146 (MQ=255)
                                                                                                                                          gcgcgTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTGGCGGTgcgcg  >  1:119446/1‑149 (MQ=255)
                                                                                                                                            gcgTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTg            >  8:180127/1‑137 (MQ=255)
                                                                                                                                            gcgTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTg            <  7:180127/137‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
CCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAGGTCTATGCGGCGGTGTACCGGGAGCGGCACGTGGCGGGGCACTTGTCCCGGGTGTACTTGAGCGTGATGCGGGCGTTGGCGGTGCGCG  >  NC_002947/2310999‑2311285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑