Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,329,762	A→C	6.9%	I39L (ATC→CTC)	PP_2048 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,329,762	0	A	C	6.9%	182.2 / 3.5	58	I39L (ATC→CTC)	PP_2048	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (25/29);  new base C (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AAGCGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCT  >  NC_002947/2329616‑2329909                                                                                                                                                   |                                                                                                                                                    aaGCGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATc                                                                                                                                                   >  1:181312/1‑149 (MQ=255) aaGCGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATc                                                                                                                                                   >  5:176319/1‑149 (MQ=255)  aGCGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATcc                                                                                                                                                  <  4:291755/149‑1 (MQ=255)   gCGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGATCTGTTCGACCTGAGCGGACATTGCCAGCAAGTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCt                                                                                                                                                 <  2:262279/149‑1 (MQ=255)   gCGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGACATCCt                                                                                                                                                 >  1:44715/1‑149 (MQ=255)        tCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTgag                                                                                                                                             <  6:147029/148‑1 (MQ=255)           aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGGATGAACTGTTCGACCTGAGCGGACATTGCCAGCACCTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGaggagg                                                                                                                                         >  8:235339/1‑149 (MQ=255)           aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCaaggatg                                                                                                                                         >  4:121322/1‑147 (MQ=255)           aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTACATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGaggagg                                                                                                                                         <  3:233282/149‑1 (MQ=255)                 gTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                   <  5:155942/149‑1 (MQ=255)                  tGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcga                                                                                                                                  <  3:121322/149‑5 (MQ=255)                  tGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACg                                                                                                                                  >  6:28977/1‑149 (MQ=255)                          ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCAc                                                                                                                            <  7:17258/147‑1 (MQ=255)                          ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCAc                                                                                                                            >  8:17258/1‑147 (MQ=255)                             aGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGc                                                                                                                        <  2:181312/148‑1 (MQ=255)                             aGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGACATCCTTGAGGAGGCCGCACGTTTCACTGGc                                                                                                                        <  2:44715/148‑1 (MQ=255)                             aGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCCCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGcc                                                                                                                       <  1:228718/149‑1 (MQ=255)                                     aCTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGatcatc                                                                                                               <  6:142017/149‑1 (MQ=255)                                             gcgcCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGc                                                                                                        >  7:290549/1‑148 (MQ=255)                                                  ccTTCGCGACATGCGTTTCGTGCGGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                   <  5:143989/116‑1 (MQ=255)                                                  ccTTCGCGACATGCGTTTCGTGCAGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                   >  6:143989/1‑116 (MQ=255)                                                   cTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGc                                                                                                             <  4:259410/137‑1 (MQ=255)                                                   cTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGc                                                                                                             >  3:259410/1‑137 (MQ=255)                                                            aTGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGa                                                                                        <  5:28977/149‑1 (MQ=255)                                                             tGCGTTTCGTGCTGGATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCg                                                                                                         >  6:180585/1‑131 (MQ=255)                                                             tGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCg                                                                                                         <  5:180585/131‑1 (MQ=255)                                                                 tttCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcgag                                                                                                                                 >  8:209831/1‑98 (MQ=255)                                                                 tttCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcgag                                                                                                                                 <  7:209831/103‑6 (MQ=255)                                                                   tCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAg                                                                                 >  5:251010/1‑149 (MQ=255)                                                                       gctgcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCTCTCAATCGCAACAGTGACGAACAgg                                                                                <  8:290549/146‑1 (MQ=255)                                                                          gcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                       >  6:260210/1‑136 (MQ=255)                                                                          gcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                       <  5:260210/136‑1 (MQ=255)                                                                           cATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGt                                                                           >  1:283114/1‑147 (MQ=255)                                                                            aTGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCACCAGTGACGAACAGGGCTGTCAg                                                                        >  2:83679/1‑149 (MQ=255)                                                                               aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  1:83679/149‑1 (MQ=255)                                                                               aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  1:37897/149‑1 (MQ=255)                                                                                aCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  7:235339/148‑1 (MQ=255)                                                                                        aCCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGGCACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGt                                                            >  1:65249/1‑149 (MQ=255)                                                                                          cTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGa                                                          <  2:305341/149‑1 (MQ=255)                                                                                             aGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAg                                                         <  6:251010/147‑1 (MQ=255)                                                                                                        ccAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAg                                                         <  8:134492/136‑1 (MQ=255)                                                                                                        ccAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAg                                                         >  7:134492/1‑136 (MQ=255)                                                                                                                 cGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGc                                   <  2:283114/149‑1 (MQ=255)                                                                                                                     aTCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     >  3:70529/1‑111 (MQ=255)                                                                                                                     aTCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  4:70529/111‑1 (MQ=255)                                                                                                                        gAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            <  4:139570/149‑1 (MQ=255)                                                                                                                        gAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            >  3:194567/1‑149 (MQ=255)                                                                                                                         aaCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            >  3:94213/1‑148 (MQ=255)                                                                                                                         aaCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            <  4:94213/148‑1 (MQ=255)                                                                                                                              gACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgt                                                                       <  4:268539/100‑1 (MQ=255)                                                                                                                              gACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgt                                                                       >  3:268539/1‑100 (MQ=255)                                                                                                                                 cgcgACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATAATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTCTCGGc                    >  1:281072/1‑148 (MQ=255)                                                                                                                                   cgACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCaa                                                                                              <  6:54762/72‑1 (MQ=255)                                                                                                                                   cgACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCaa                                                                                              >  5:54762/1‑72 (MQ=255)                                                                                                                                         ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgggtgg                                                                 <  6:9957/95‑1 (MQ=255)                                                                                                                                         ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgggtgg                                                                 >  5:9957/1‑95 (MQ=255)                                                                                                                                         ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGAGGGCGGTGAGGTCCCAACCCCGGACGGCAGGCTGGACGCCTATCTGCAATACGTc            >  5:157587/1‑148 (MQ=255)                                                                                                                                                  cATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCt  >  3:136774/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AAGCGTTTCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCT  >  NC_002947/2329616‑2329909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑