Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,329,767 T→C 7.3% L40L (CTT→CTC)  PP_2048 → acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,329,7670TC 7.3% 159.1 / 2.7 55L40L (CTT→CTC) PP_2048acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (23/28);  new base C (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

TCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCT  >  NC_002947/2329623‑2329909
                                                                                                                                                |                                                                                                                                              
tCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTgag                                                                                                                                             <  6:147029/148‑1 (MQ=255)
   aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGGATGAACTGTTCGACCTGAGCGGACATTGCCAGCACCTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGaggagg                                                                                                                                         >  8:235339/1‑149 (MQ=255)
   aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCaaggatg                                                                                                                                         >  4:121322/1‑147 (MQ=255)
   aaCCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTACATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGaggagg                                                                                                                                         <  3:233282/149‑1 (MQ=255)
         gTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                   <  5:155942/149‑1 (MQ=255)
          tGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcga                                                                                                                                  <  3:121322/149‑5 (MQ=255)
          tGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACg                                                                                                                                  >  6:28977/1‑149 (MQ=255)
                  ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCAc                                                                                                                            >  8:17258/1‑147 (MQ=255)
                  ttCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCAc                                                                                                                            <  7:17258/147‑1 (MQ=255)
                     aGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGc                                                                                                                        <  2:181312/148‑1 (MQ=255)
                     aGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGACATCCTTGAGGAGGCCGCACGTTTCACTGGc                                                                                                                        <  2:44715/148‑1 (MQ=255)
                     aGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCCCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGcc                                                                                                                       <  1:228718/149‑1 (MQ=255)
                             aCTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGatcatc                                                                                                               <  6:142017/149‑1 (MQ=255)
                                     gcgcCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGc                                                                                                        >  7:290549/1‑148 (MQ=255)
                                          ccTTCGCGACATGCGTTTCGTGCGGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                   <  5:143989/116‑1 (MQ=255)
                                          ccTTCGCGACATGCGTTTCGTGCAGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCAc                                                                                                                                   >  6:143989/1‑116 (MQ=255)
                                           cTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGc                                                                                                             >  3:259410/1‑137 (MQ=255)
                                           cTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGc                                                                                                             <  4:259410/137‑1 (MQ=255)
                                                    aTGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGa                                                                                        <  5:28977/149‑1 (MQ=255)
                                                     tGCGTTTCGTGCTGGATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCg                                                                                                         >  6:180585/1‑131 (MQ=255)
                                                     tGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCg                                                                                                         <  5:180585/131‑1 (MQ=255)
                                                         tttCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcgag                                                                                                                                 <  7:209831/103‑6 (MQ=255)
                                                         tttCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCCTCCTCAAGGATGCCgtcgag                                                                                                                                 >  8:209831/1‑98 (MQ=255)
                                                           tCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAg                                                                                 >  5:251010/1‑149 (MQ=255)
                                                               gctgcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCTCTCAATCGCAACAGTGACGAACAgg                                                                                <  8:290549/146‑1 (MQ=255)
                                                                  gcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                       >  6:260210/1‑136 (MQ=255)
                                                                  gcATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGAc                                                                                       <  5:260210/136‑1 (MQ=255)
                                                                   cATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGt                                                                           >  1:283114/1‑147 (MQ=255)
                                                                    aTGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCACCAGTGACGAACAGGGCTGTCAg                                                                        >  2:83679/1‑149 (MQ=255)
                                                                       aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  1:83679/149‑1 (MQ=255)
                                                                       aaCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  1:37897/149‑1 (MQ=255)
                                                                        aCTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  7:235339/148‑1 (MQ=255)
                                                                                aCCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGGCACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGt                                                            >  1:65249/1‑149 (MQ=255)
                                                                                  cTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGa                                                          <  2:305341/149‑1 (MQ=255)
                                                                                     aGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAg                                                         <  6:251010/147‑1 (MQ=255)
                                                                                                ccAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAg                                                         <  8:134492/136‑1 (MQ=255)
                                                                                                ccAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAg                                                         >  7:134492/1‑136 (MQ=255)
                                                                                                         cGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGc                                   <  2:283114/149‑1 (MQ=255)
                                                                                                             aTCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     >  3:70529/1‑111 (MQ=255)
                                                                                                             aTCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgg                                                                     <  4:70529/111‑1 (MQ=255)
                                                                                                                gAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            <  4:139570/149‑1 (MQ=255)
                                                                                                                gAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            >  3:194567/1‑149 (MQ=255)
                                                                                                                 aaCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            <  4:94213/148‑1 (MQ=255)
                                                                                                                 aaCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGc                            >  3:94213/1‑148 (MQ=255)
                                                                                                                      gACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgt                                                                       >  3:268539/1‑100 (MQ=255)
                                                                                                                      gACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgt                                                                       <  4:268539/100‑1 (MQ=255)
                                                                                                                         cgcgACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATAATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTCTCGGc                    >  1:281072/1‑148 (MQ=255)
                                                                                                                           cgACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCaa                                                                                              <  6:54762/72‑1 (MQ=255)
                                                                                                                           cgACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCaa                                                                                              >  5:54762/1‑72 (MQ=255)
                                                                                                                                 ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgggtgg                                                                 <  6:9957/95‑1 (MQ=255)
                                                                                                                                 ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgggtgg                                                                 >  5:9957/1‑95 (MQ=255)
                                                                                                                                 ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGAGGGCGGTGAGGTCCCAACCCCGGACGGCAGGCTGGACGCCTATCTGCAATACGTc            >  5:157587/1‑148 (MQ=255)
                                                                                                                                          cATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCt  >  3:136774/1‑149 (MQ=255)
                                                                                                                                            tCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgggt                                                                   >  3:40311/1‑82 (MQ=255)
                                                                                                                                            tCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAgtgggt                                                                   <  4:40311/82‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TCGAACCGCGTGGGGAGGTTCAGGCATGAACTACCAGGCGCCCCTTCGCGACATGCGTTTCGTGCTGCATGAACTGTTCGACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCT  >  NC_002947/2329623‑2329909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑