Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,332,944 T→G 9.8% C109G (TGT→GGT)  PP_2050 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,332,9440TG 9.8% 103.0 / 3.9 41C109G (TGT→GGT) PP_2050hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/18);  new base G (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

GCGCTGGTGGTCAGCTCCGCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGC  >  NC_002947/2332800‑2333091
                                                                                                                                                |                                                                                                                                                   
gcgcTGGTGGTCAGCTCCGCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGc                                                                                                                                                 <  6:185569/149‑1 (MQ=255)
             gCTCCTCCTGCTAGTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGTCCAGCCACTGGCCTGTGCCACCGGCCGCTTc                                                                                                                                    <  4:160712/149‑1 (MQ=255)
                 cGCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGt                                                                                                                                  <  6:56435/147‑1 (MQ=255)
                  gCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCa                                                                                                                               <  7:285856/149‑1 (MQ=255)
                             ccAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATtcgtc                                                                                                                     >  6:131258/1‑148 (MQ=255)
                                          gCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggcg                                                                                                       >  5:178313/1‑149 (MQ=255)
                                          gCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggcg                                                                                                       >  6:282721/1‑149 (MQ=255)
                                          gCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggc                                                                                                        <  5:131258/148‑1 (MQ=255)
                                           ccTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggcgg                                                                                                      >  3:299553/1‑149 (MQ=255)
                                              tCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGt                                                                                                   >  2:4260/1‑149 (MQ=255)
                                                    aTGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTccggtg                                                                                             >  5:52596/1‑149 (MQ=255)
                                                      gACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCt                                                                                                                                                     >  8:1911/1‑91 (MQ=255)
                                                      gACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCt                                                                                                                                                     <  7:1911/91‑1 (MQ=255)
                                                                                ccGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATgtgt                                                                 >  4:236817/1‑149 (MQ=255)
                                                                                 cGGTTGGGCAACACGTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGtt                                                                <  1:4260/149‑1 (MQ=255)
                                                                                  ggTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTg                                                               <  6:178313/149‑1 (MQ=255)
                                                                                   gTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGa                                                              <  6:52596/149‑1 (MQ=255)
                                                                                   gTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGa                                                              <  5:282721/149‑1 (MQ=255)
                                                                                       ggCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCg                                                           >  8:103404/1‑148 (MQ=255)
                                                                                          aaCAGCTCGGTGCACTATCAGTTGGCGGTGTTCCTGTCCGGCCAGCCTCTGGCCTGTGCGACCGGCCGCTTCTTCCATCTCTTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACt                                                       <  4:252366/149‑1 (MQ=255)
                                                                                                       aCTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATcc                                          >  7:139325/1‑149 (MQ=255)
                                                                                                        cTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCct                                         >  8:97919/1‑149 (MQ=255)
                                                                                                         tATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCctc                                        >  4:282073/1‑149 (MQ=255)
                                                                                                                            tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGa                                     <  1:311/133‑1 (MQ=255)
                                                                                                                            tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGa                                     >  2:311/1‑133 (MQ=255)
                                                                                                                            tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATg                      >  1:235288/1‑148 (MQ=255)
                                                                                                                            tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATg                      >  3:138007/1‑148 (MQ=255)
                                                                                                                                cGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                       <  5:253302/143‑1 (MQ=255)
                                                                                                                                cGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                       >  6:253302/1‑143 (MQ=255)
                                                                                                                                cGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGaaa                   <  4:299553/147‑1 (MQ=255)
                                                                                                                                     agccaCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCg            <  7:97919/149‑1 (MQ=255)
                                                                                                                                      gatttcggCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGCCCAGCCATCATGAAAGATGCCGt           <  7:199204/143‑1 (MQ=255)
                                                                                                                                       ccaCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGt           >  3:228392/1‑148 (MQ=255)
                                                                                                                                        caCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                       <  5:78254/135‑1 (MQ=255)
                                                                                                                                        caCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                       >  6:78254/1‑135 (MQ=255)
                                                                                                                                         aCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCt                                                                            <  2:123599/81‑1 (MQ=255)
                                                                                                                                         aCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCt                                                                            >  1:123599/1‑81 (MQ=255)
                                                                                                                                          gcggCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTACAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtc       >  3:146067/3‑149 (MQ=255)
                                                                                                                                          cTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtc       <  3:236817/149‑1 (MQ=255)
                                                                                                                                            ggCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGGACAGCCATCATGAAAGATGCCGt           >  8:199204/1‑143 (MQ=255)
                                                                                                                                             gCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtcgt     <  7:103404/148‑1 (MQ=255)
                                                                                                                                             gCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtcgtc    <  3:284303/149‑1 (MQ=255)
                                                                                                                                               cTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGc  >  6:312818/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCGCTGGTGGTCAGCTCCGCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGC  >  NC_002947/2332800‑2333091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑