Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,332,952 C→A 8.9% T111T (ACC→ACA)  PP_2050 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,332,9520CA 8.9% 122.3 / 4.0 45T111T (ACC→ACA) PP_2050hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/19);  new base A (2/2);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

GCTCCGCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGCCACTG  >  NC_002947/2332813‑2333096
                                                                                                                                           |                                                                                                                                                
gCTCCTCCTGCTAGTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGTCCAGCCACTGGCCTGTGCCACCGGCCGCTTc                                                                                                                                         <  4:160712/149‑1 (MQ=255)
    cGCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGt                                                                                                                                       <  6:56435/147‑1 (MQ=255)
     gCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCa                                                                                                                                    <  7:285856/149‑1 (MQ=255)
                ccAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATtcgtc                                                                                                                          >  6:131258/1‑148 (MQ=255)
                             gCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggcg                                                                                                            >  5:178313/1‑149 (MQ=255)
                             gCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggcg                                                                                                            >  6:282721/1‑149 (MQ=255)
                             gCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggc                                                                                                             <  5:131258/148‑1 (MQ=255)
                              ccTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGggcgg                                                                                                           >  3:299553/1‑149 (MQ=255)
                                 tCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGt                                                                                                        >  2:4260/1‑149 (MQ=255)
                                       aTGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTccggtg                                                                                                  >  5:52596/1‑149 (MQ=255)
                                                                   ccGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATgtgt                                                                      >  4:236817/1‑149 (MQ=255)
                                                                    cGGTTGGGCAACACGTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGtt                                                                     <  1:4260/149‑1 (MQ=255)
                                                                     ggTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTg                                                                    <  6:178313/149‑1 (MQ=255)
                                                                      gTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGa                                                                   <  5:282721/149‑1 (MQ=255)
                                                                      gTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGa                                                                   <  6:52596/149‑1 (MQ=255)
                                                                          ggCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCg                                                                >  8:103404/1‑148 (MQ=255)
                                                                             aaCAGCTCGGTGCACTATCAGTTGGCGGTGTTCCTGTCCGGCCAGCCTCTGGCCTGTGCGACCGGCCGCTTCTTCCATCTCTTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACt                                                            <  4:252366/149‑1 (MQ=255)
                                                                                          aCTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATcc                                               >  7:139325/1‑149 (MQ=255)
                                                                                           cTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCct                                              >  8:97919/1‑149 (MQ=255)
                                                                                            tATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCctc                                             >  4:282073/1‑149 (MQ=255)
                                                                                                               tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGa                                          >  2:311/1‑133 (MQ=255)
                                                                                                               tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGa                                          <  1:311/133‑1 (MQ=255)
                                                                                                               tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATg                           >  1:235288/1‑148 (MQ=255)
                                                                                                               tctcCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATg                           >  3:138007/1‑148 (MQ=255)
                                                                                                                   cGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                            <  5:253302/143‑1 (MQ=255)
                                                                                                                   cGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                            >  6:253302/1‑143 (MQ=255)
                                                                                                                   cGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGaaa                        <  4:299553/147‑1 (MQ=255)
                                                                                                                        agccaCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCg                 <  7:97919/149‑1 (MQ=255)
                                                                                                                         gatttcggCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGCCCAGCCATCATGAAAGATGCCGt                <  7:199204/143‑1 (MQ=255)
                                                                                                                          ccaCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGt                >  3:228392/1‑148 (MQ=255)
                                                                                                                           caCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                            <  5:78254/135‑1 (MQ=255)
                                                                                                                           caCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCcatcat                            >  6:78254/1‑135 (MQ=255)
                                                                                                                            aCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCt                                                                                 >  1:123599/1‑81 (MQ=255)
                                                                                                                            aCTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCt                                                                                 <  2:123599/81‑1 (MQ=255)
                                                                                                                             gcggCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTACAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtc            >  3:146067/3‑149 (MQ=255)
                                                                                                                             cTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtc            <  3:236817/149‑1 (MQ=255)
                                                                                                                               ggCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGGACAGCCATCATGAAAGATGCCGt                >  8:199204/1‑143 (MQ=255)
                                                                                                                                gCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtcgt          <  7:103404/148‑1 (MQ=255)
                                                                                                                                gCCGGTGGCACAGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtcgtc         <  3:284303/149‑1 (MQ=255)
                                                                                                                                  cTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGc       >  6:312818/1‑149 (MQ=255)
                                                                                                                                    gtgCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtcg           >  7:204324/1‑143 (MQ=255)
                                                                                                                                    gtgCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGAtcg           <  8:204324/143‑1 (MQ=255)
                                                                                                                                     tgCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGCCa     <  2:235288/148‑1 (MQ=255)
                                                                                                                                       ccACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGCCACTg  >  5:183874/1‑149 (MQ=255)
                                                                                                                                       ccACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGCCACTg  >  5:32340/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                
GCTCCGCCTGCGACTACCAGGCGCCGGTGGCCTTCCCGCATGACATCGAAGTGGGCCTTGCGGTGAGCCGGTTGGGCAACACCTCGGTGCACTATCAGTTGGCGGTGTTCCTCTCCGGCCAGCCACTGGCCTGTGCCACCGGCCGCTTCGTCCATGTATTCGTCGACCGCGAAGGGCGGCGTCCGGTGCCGGTGCCTGACTGCCTACGGCATGTGTTGAGCGAACTGCAGCCCCTATCCTCTGAGGAACAGCCATCATGAAAGATGCCGTGATCGTCGCCACTG  >  NC_002947/2332813‑2333096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑