Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,367,410 A→C 7.1% Q76H (CAA→CAC)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,367,4100AC 7.1% 177.2 / 4.6 56Q76H (CAA→CAC) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base C (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CCTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAA  >  NC_002947/2367266‑2367557
                                                                                                                                                |                                                                                                                                                   
ccTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGc                                                                                                                                                 >  4:269879/1‑149 (MQ=255)
 cTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCg                                                                                                                                                >  5:250898/1‑149 (MQ=255)
      acaAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgtgt                                                                                                                                           <  4:248719/149‑1 (MQ=255)
         aGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcg                                                                                                                                        <  3:256481/149‑6 (MQ=255)
          gTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACaa                                                                                                                                       <  8:76044/149‑1 (MQ=255)
            ggCGCTGTTCGCCGAGGAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcggg                                                                                                                                      <  4:209572/148‑8 (MQ=255)
              cgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCt                                                                                                                                   <  3:269879/149‑1 (MQ=255)
                 tGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGAt                                                                                                                                >  1:208169/1‑149 (MQ=255)
                 tGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGAt                                                                                                                                >  2:225842/1‑149 (MQ=255)
                  gTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGAt                                                                                                                                >  6:149014/1‑148 (MQ=255)
                   ttCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCTTCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTa                                                                                                                              <  1:87608/149‑1 (MQ=255)
                       ccGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGaa                                                                                                                          >  1:116011/1‑149 (MQ=255)
                           gCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAAcc                                                                                                                                     >  4:300888/1‑134 (MQ=255)
                           gCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAAcc                                                                                                                                     <  3:300888/134‑1 (MQ=255)
                              gTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtg                                                                                                                   >  2:2096/1‑149 (MQ=255)
                                    cGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctg                                                                                                               <  1:96960/147‑1 (MQ=255)
                                    cGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggct                                                                                                                <  7:147215/146‑1 (MQ=255)
                                              tctctGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGc                                                                                                                       <  5:133958/129‑1 (MQ=255)
                                              tctctGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGc                                                                                                                       >  6:133958/1‑129 (MQ=255)
                                                  tGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGTGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATAcc                                                                                               >  3:91900/1‑149 (MQ=255)
                                                  tGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGa                                                                                                                                 >  3:208092/1‑115 (MQ=255)
                                                  tGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGa                                                                                                                                 <  4:208092/115‑1 (MQ=255)
                                                       gAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGt                                                                                          >  6:100577/1‑149 (MQ=255)
                                                           tCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCg                                                                                        >  6:14875/1‑147 (MQ=255)
                                                              cccAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtg                                                                                   >  2:98759/1‑149 (MQ=255)
                                                                   cgcAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCTGTCGACCCATACCGTGGTCGAAGtgctgct                                                                              <  2:116011/149‑2 (MQ=255)
                                                                          tttCCGACCTGGCCGGCTGCCCCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                       >  6:226743/1‑149 (MQ=255)
                                                                            tCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGTGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                       <  4:91900/147‑1 (MQ=255)
                                                                            tCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAAGGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                       <  4:34628/147‑1 (MQ=255)
                                                                                aCCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCg                                                                 >  7:51383/1‑149 (MQ=255)
                                                                                    ggCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTcc                                                             >  2:112353/1‑149 (MQ=255)
                                                                                     gCCGGCTGCACCCTGTCATCCTGGCGCATCATCGATCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTc                                                              <  5:161525/147‑1 (MQ=255)
                                                                                     gCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTc                                                              <  5:100577/147‑1 (MQ=255)
                                                                                              aCCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCAc                                                                             <  7:140180/123‑1 (MQ=255)
                                                                                              aCCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCAc                                                                             >  8:140180/1‑123 (MQ=255)
                                                                                                ccTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCgg                                                 >  1:130201/1‑149 (MQ=255)
                                                                                                 cTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGt                                                <  1:178796/149‑1 (MQ=255)
                                                                                                    tCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTgcg                                             >  3:297369/1‑149 (MQ=255)
                                                                                                               catcatCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAgtcga                                  <  1:225842/149‑5 (MQ=255)
                                                                                                                         cGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTgg                        <  5:14875/149‑1 (MQ=255)
                                                                                                                          gTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGc                       <  8:51383/149‑1 (MQ=255)
                                                                                                                             tCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTa                     <  5:149014/148‑1 (MQ=255)
                                                                                                                                aCCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGt                                                         <  2:66657/109‑1 (MQ=255)
                                                                                                                                aCCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGt                                                         >  1:66657/1‑109 (MQ=255)
                                                                                                                                 ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGa                                                      <  8:184792/111‑1 (MQ=255)
                                                                                                                                 ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGa                                                      >  7:184792/1‑111 (MQ=255)
                                                                                                                                  cccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGc                       >  2:194467/1‑141 (MQ=255)
                                                                                                                                  cccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGc                       <  1:194467/141‑1 (MQ=255)
                                                                                                                                   ccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAATTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATc              <  1:98759/149‑1 (MQ=255)
                                                                                                                                     ttacaCCCGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACACGGTGCGAACCGAGCTCAAACGCCGTGGCTACAGCATccc            >  6:268842/5‑148 (MQ=255)
                                                                                                                                     gAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTa                     <  4:184200/140‑1 (MQ=255)
                                                                                                                                     gAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTa                     >  3:184200/1‑140 (MQ=255)
                                                                                                                                        caCCCGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCacac         >  3:83277/2‑149 (MQ=255)
                                                                                                                                           ccGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCt      >  7:261535/1‑149 (MQ=255)
                                                                                                                                              cAAGTGCGGGTGTACAACCCTGATTAGGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTg                                                           <  3:149907/93‑1 (MQ=255)
                                                                                                                                              cAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACAATGACCTGCCCTTCATg                                                           >  4:149907/1‑93 (MQ=255)
                                                                                                                                               aaGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCaa  <  2:208169/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CCTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAA  >  NC_002947/2367266‑2367557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑