Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,417,286	C→G	6.5%	H107Q (CAC→CAG)	PP_2119 →	ABC transporter permease/ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,417,286	0	C	G	6.5%	192.7 / 3.9	62	H107Q (CAC→CAG)	PP_2119	ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (23/35);  new base G (2/2);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CCGCTCCGCGCACCAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGTTCCTGCGCAACGCGCTGATGGTCATTGGCGGTG  >  NC_002947/2417139‑2417434                                                                                                                                                     |                                                                                                                                                     ccGCTCCGCGCACCAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCAcc                                                                                                                                                     <  6:55234/149‑1 (MQ=255) ccGCTCCGCGCACCAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCAcc                                                                                                                                                     >  4:91585/1‑149 (MQ=255) ccGCTCCGCGCACCAGCTCAATCAGACCATCGGGCTGTTACTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCAcc                                                                                                                                                     >  4:109889/1‑149 (MQ=255)    cTCCGCGCACCAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGa                                                                                                                                                  <  1:62029/149‑1 (MQ=255)         cgcACCAGCTCAATGAGACGTTCTGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGTCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATc                                                                                                                                                <  2:158697/146‑1 (MQ=255)            aCCAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTg                                                                                                                                          >  5:147317/1‑149 (MQ=255)             ccAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTg                                                                                                                                          >  8:94310/1‑148 (MQ=255)              ccgCTCACTCCGAGCTTAGGGCTGTTCCTGATGCTGGGGCTGGCTCTGGGGGTGGGTACCTTCTGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGATCGTTGTGTGGCGGATTTCCGTCGTGCGGTCTTCGATCAGGTGATCgaaga                                                                                                                                           <  8:199808/144‑5 (MQ=255)              cAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCAGGTGATCgaagacca                                                                                                                                        >  7:265622/1‑142 (MQ=255)              cAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCAGGTGATCgaagac                                                                                                                                          <  8:265622/147‑6 (MQ=255)              cAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGG‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCAGGTGATCgatga                                                                                                                                           >  7:199808/1‑142 (MQ=255)                gCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGc                                                                                                                                             >  2:81169/1‑142 (MQ=255)                gCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGc                                                                                                                                             <  1:81169/142‑1 (MQ=255)                    aaTCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAgg                                                                                                                                  >  2:143401/1‑149 (MQ=255)                    aaTCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAg                                                                                                                                   <  4:286829/148‑1 (MQ=255)                     aTCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGt                                                                                                                                 <  6:208119/149‑1 (MQ=255)                         gACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGttttt                                                                                                                             >  8:202771/1‑149 (MQ=255)                          aCCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGtttttt                                                                                                                            <  3:109889/149‑1 (MQ=255)                          aCCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGtttttt                                                                                                                            <  3:91585/149‑1 (MQ=255)                            cATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTc                                                                                                                           >  4:203027/1‑148 (MQ=255)                                gggCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGa                                                                                                                      <  3:307413/149‑1 (MQ=255)                                 ggCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGAc                                                                                                                     <  5:235967/149‑1 (MQ=255)                                      ttCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCTGGTTTTTTCGAGGACAACCg                                                                                                                >  6:313550/1‑149 (MQ=255)                                      ttCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAAc                                                                                                                  <  3:207148/147‑1 (MQ=255)                                       tCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCg                                                                                                                >  7:24528/1‑148 (MQ=255)                                           gctgctgGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCt                                                                                                           >  2:87666/1‑149 (MQ=255)                                                     ctggcgctggcgGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATc                                                                                                   >  8:25387/1‑147 (MQ=255)                                                     ctggcgctggcgGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCa                                                                                                 <  7:172047/149‑1 (MQ=255)                                                      tggcgctggcgGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGTAGGATTGGCGAGCGTTTTGTGGCCGATAACCTTCTTGCGGTATTCGATCACATGATCGGCCTGCACCAAGGTTTTTTCGAGGAAAACCGCAGATCCGATATCCa                                                                                                 >  1:65881/1‑149 (MQ=255)                                                        gcgctggcgGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGtc                                                                                              >  4:144954/1‑149 (MQ=255)                                                             ggcgGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGc                                                                                         <  7:25387/149‑1 (MQ=255)                                                               cgGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTg                                                                                       <  3:255003/149‑1 (MQ=255)                                                                ggTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGa                                                                                      <  2:245525/149‑1 (MQ=255)                                                                     gTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCg                                                                                 <  6:147317/149‑1 (MQ=255)                                                                            cAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGacac                                                                            <  1:143401/147‑1 (MQ=255)                                                                            cAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGacac                                                                            <  3:144954/147‑1 (MQ=255)                                                                             aGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGAcacca                                                                          <  8:24528/148‑1 (MQ=255)                                                                                        tCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCga                                                                                                       >  2:225841/1‑108 (MQ=255)                                                                                        tCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCAGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCga                                                                                                       <  1:225841/108‑1 (MQ=255)                                                                                           ggTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAg                                                           >  3:27156/1‑149 (MQ=255)                                                                                            gtgtCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAg                                                           <  2:65881/148‑1 (MQ=255)                                                                                                cGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATc                                                      <  4:237494/149‑1 (MQ=255)                                                                                                    ggATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGt                                                   >  6:153075/1‑149 (MQ=255)                                                                                                    ggATTGGCGAGCGTTGTGAGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGt                                                   >  6:5453/1‑149 (MQ=255)                                                                                                     gATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGt                                                   <  5:153075/148‑1 (MQ=255)                                                                                                     gATTGGCGAGCGTTGTGGGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGt                                                   <  5:5453/148‑1 (MQ=255)                                                                                                       ttGGCGAGCGTTGTGTGGCCGATATCGGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTc                                                 <  8:103600/148‑1 (MQ=255)                                                                                                        tGGCTAGCGTTGTTTGGCCGATATCCGTCGTGCTGTCTTCGATCACCTGATAGGGCTGCACCCAGTTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGa                                                                               <  5:239586/117‑1 (MQ=255)                                                                                                         ggCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCAt                                               <  1:87666/148‑1 (MQ=255)                                                                                                         ggCGAGCGGTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCAt                                               <  7:202771/148‑1 (MQ=255)                                                                                                          gCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCaa                                                               >  1:213712/1‑131 (MQ=255)                                                                                                          gCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCaa                                                               <  2:213712/131‑1 (MQ=255)                                                                                                            gAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTTATCGCt                                           >  5:170736/1‑149 (MQ=255)                                                                                                            gAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCt                                           >  8:47422/1‑149 (MQ=255)                                                                                                            gAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCt                                           >  5:275527/1‑149 (MQ=255)                                                                                                                     gtgGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGtt                                  <  3:203027/149‑1 (MQ=255)                                                                                                                      tgGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGTTc                                 <  6:275527/149‑1 (MQ=255)                                                                                                                        gCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGTTCCt                               <  6:169354/149‑1 (MQ=255)                                                                                                                          cGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAAt                                                              <  8:85407/116‑1 (MQ=255)                                                                                                                          cGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAAt                                                              >  7:85407/1‑116 (MQ=255)                                                                                                                               tCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCTGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGTTCCTGCGCAAc                        <  5:313550/149‑1 (MQ=255)                                                                                                                                 cgtcgtGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGTGGACACCACCTTGCTGCAATCAGt                                                          >  4:204257/1‑113 (MQ=255)                                                                                                                                 cgtcgtGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGTGGACACCACCTTGCTGCAATCAGt                                                          <  3:204257/113‑1 (MQ=255)                                                                                                                                      tGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTTATCGCTGTCGATGTTCCTGCGCAACGCGCTGa                 <  6:170736/149‑1 (MQ=255)                                                                                                                                             ttCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGTTCCTGCGCAACGCGCTGATGGTCAt          >  4:263037/1‑149 (MQ=255)                                                                                                                                                     ccTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGTTCCTGCGCAACGCGCTGATGGTCATTGGCGgtg  <  3:263037/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCGCTCCGCGCACCAGCTCAATCAGACCATCGGGCTGTTCCTGCTGCTGGTGCTGGCGCTGGCGGTGGGTACCTTCAGCCGTTTCTATCTGGTGTCGT‑GGATTGGCGAGCGTTGTGTGGCCGATATCCGTCGTGCGGTCTTCGATCACCTGATCGGCCTGCACCCAGGTTTTTTCGAGGACAACCGCAGCTCCGAGATCCAGTCTCGGCTGACTGCGGACACCACCTTGCTGCAATCAGTGATCGGTTCATCGCTGTCGATGTTCCTGCGCAACGCGCTGATGGTCATTGGCGGTG  >  NC_002947/2417139‑2417434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑