Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,419,298 C→A 10.6% A150E (GCG→GAG) ‡ ctpH → methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,419,2980CA10.6% 138.3 / 6.4 47A150E (GCG→GAG) ‡ctpHmethyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand):  ref base C (20/22);  new base A (2/3);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TGGCAGCAGTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGCCAGTGCCGAG  >  NC_002947/2419150‑2419446
                                                                                                                                                    |                                                                                                                                                    
tGGCAGCAGTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTAGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGc                                                                                                                                                      >  2:196103/1‑149 (MQ=255)
      cagTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCt                                                                                                                                                <  7:84271/149‑1 (MQ=255)
      cagTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCt                                                                                                                                                >  6:299480/1‑149 (MQ=255)
       agTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTg                                                                                                                                               <  4:298563/149‑1 (MQ=255)
         ttCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTggc                                                                                                                                             <  3:28471/149‑1 (MQ=255)
         ttCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTggc                                                                                                                                             >  3:95213/1‑149 (MQ=255)
            cAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcgg                                                                                                                                           <  1:283588/148‑1 (MQ=255)
              ggCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAg                                                                                                                                        >  1:39542/1‑149 (MQ=255)
               gCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAgg                                                                                                                                       >  1:307029/1‑149 (MQ=255)
                cccGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcggccttg                                                                                                                                      <  6:37268/149‑5 (MQ=255)
                 ccGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGAGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAggcg                                                                                                                                     >  4:38631/1‑149 (MQ=255)
                  cGAGAACAGCAAGTTCAACATCTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAggcgg                                                                                                                                    <  1:196103/149‑1 (MQ=255)
                  cGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCg                                                                                                                                                     <  5:145426/132‑1 (MQ=255)
                  cGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCg                                                                                                                                                     >  6:145426/1‑132 (MQ=255)
                                  aacaacTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcggc                                                                                                                                          >  2:24826/1‑127 (MQ=255)
                                  aacaacTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcggc                                                                                                                                          <  1:24826/127‑1 (MQ=255)
                                      acTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGAcc                                                                                                                <  5:310324/149‑1 (MQ=255)
                                             gggCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCgg                                                                                                          >  5:2468/1‑148 (MQ=255)
                                                 aaTTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCaaa                                                                                                     <  5:299480/149‑1 (MQ=255)
                                                 aaTTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCa                                                                                                       >  1:5222/1‑147 (MQ=255)
                                                    tGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAgg                                                                                                   <  6:2468/148‑1 (MQ=255)
                                                      aTCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGt                                                                                                <  2:307029/149‑1 (MQ=255)
                                                         gACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGa                                                                                             >  4:208031/1‑149 (MQ=255)
                                                          ggcacaGCGATGCCAGGCGCACGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGAc                                                                                            <  1:149463/146‑1 (MQ=255)
                                                             tcaGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                         <  4:31065/148‑1 (MQ=255)
                                                             acaGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                         >  2:299966/1‑149 (MQ=255)
                                                              caGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTg                                                                                        <  4:147246/149‑1 (MQ=255)
                                                               aGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTgc                                                                                       >  3:240825/1‑149 (MQ=255)
                                                                        aGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCAGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGa                                                                              <  5:163436/149‑1 (MQ=255)
                                                                             cAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCaaa                                                                         >  8:260688/1‑149 (MQ=255)
                                                                              aaGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAAt                                                                        <  2:5222/149‑1 (MQ=255)
                                                                                          cagcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                         >  8:221259/1‑120 (MQ=255)
                                                                                          cagcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                         <  7:221259/120‑1 (MQ=255)
                                                                                           agcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTc                                                           <  4:95213/149‑1 (MQ=255)
                                                                                            gcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCg                                                                                                                   >  1:132067/1‑92 (MQ=255)
                                                                                            gcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCg                                                                                                                   <  2:132067/92‑1 (MQ=255)
                                                                                                              ccGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCa                                                                                  >  3:111147/1‑107 (MQ=255)
                                                                                                              ccGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCa                                                                                  <  4:111147/107‑1 (MQ=255)
                                                                                                                                cTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcagc                        >  2:275767/1‑147 (MQ=255)
                                                                                                                                 tATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcagccag                     >  1:176737/1‑149 (MQ=255)
                                                                                                                                      cgcctgccgccAGGAACGCCTGGCGGCCGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATc                 >  7:14962/6‑148 (MQ=255)
                                                                                                                                      acaAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGc                           <  5:55211/138‑1 (MQ=255)
                                                                                                                                      acaAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGc                           >  6:55211/1‑138 (MQ=255)
                                                                                                                                      acaAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATc                 >  5:294199/1‑148 (MQ=255)
                                                                                                                                          ggCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGc            <  7:260688/149‑1 (MQ=255)
                                                                                                                                          ggCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGc            <  4:240825/149‑1 (MQ=255)
                                                                                                                                             cgccAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcag                         <  3:190435/133‑1 (MQ=255)
                                                                                                                                             cgccAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcag                         >  4:190435/1‑133 (MQ=255)
                                                                                                                                                    cGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGCCAGTGCCGAg  <  3:208031/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGCAGCAGTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGCCAGTGCCGAG  >  NC_002947/2419150‑2419446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑