Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,419,301 T→G 10.6% F151C (TTC→TGC) ‡ ctpH → methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,419,3010TG10.6% 135.4 / 8.5 47F151C (TTC→TGC) ‡ctpHmethyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand):  ref base T (20/22);  new base G (2/3);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CAGTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGCCAGTGCCGAGC  >  NC_002947/2419156‑2419447
                                                                                                                                                 |                                                                                                                                                  
cagTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCt                                                                                                                                                 <  7:84271/149‑1 (MQ=255)
cagTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCt                                                                                                                                                 >  6:299480/1‑149 (MQ=255)
 agTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTg                                                                                                                                                <  4:298563/149‑1 (MQ=255)
   ttCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTggc                                                                                                                                              <  3:28471/149‑1 (MQ=255)
   ttCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTggc                                                                                                                                              >  3:95213/1‑149 (MQ=255)
      cAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcgg                                                                                                                                            <  1:283588/148‑1 (MQ=255)
        ggCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAg                                                                                                                                         >  1:39542/1‑149 (MQ=255)
         gCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAgg                                                                                                                                        >  1:307029/1‑149 (MQ=255)
          cccGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcggccttg                                                                                                                                       <  6:37268/149‑5 (MQ=255)
           ccGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGAGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAggcg                                                                                                                                      >  4:38631/1‑149 (MQ=255)
            cGAGAACAGCAAGTTCAACATCTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAggcgg                                                                                                                                     <  1:196103/149‑1 (MQ=255)
                            aacaacTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcggc                                                                                                                                           >  2:24826/1‑127 (MQ=255)
                            aacaacTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGAACGCCTggcggc                                                                                                                                           <  1:24826/127‑1 (MQ=255)
                                acTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGAcc                                                                                                                 <  5:310324/149‑1 (MQ=255)
                                       gggCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCgg                                                                                                           >  5:2468/1‑148 (MQ=255)
                                           aaTTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCaaa                                                                                                      <  5:299480/149‑1 (MQ=255)
                                           aaTTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCa                                                                                                        >  1:5222/1‑147 (MQ=255)
                                              tGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAgg                                                                                                    <  6:2468/148‑1 (MQ=255)
                                                aTCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGt                                                                                                 <  2:307029/149‑1 (MQ=255)
                                                   gACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGa                                                                                              >  4:208031/1‑149 (MQ=255)
                                                    ggcacaGCGATGCCAGGCGCACGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGAc                                                                                             <  1:149463/146‑1 (MQ=255)
                                                       tcaGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                          <  4:31065/148‑1 (MQ=255)
                                                       acaGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                          >  2:299966/1‑149 (MQ=255)
                                                        caGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTg                                                                                         <  4:147246/149‑1 (MQ=255)
                                                         aGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTgc                                                                                        >  3:240825/1‑149 (MQ=255)
                                                                  aGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCAGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGa                                                                               <  5:163436/149‑1 (MQ=255)
                                                                       cAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCaaa                                                                          >  8:260688/1‑149 (MQ=255)
                                                                        aaGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAAt                                                                         <  2:5222/149‑1 (MQ=255)
                                                                                    cagcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                          >  8:221259/1‑120 (MQ=255)
                                                                                    cagcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGt                                                                                          <  7:221259/120‑1 (MQ=255)
                                                                                     agcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTc                                                            <  4:95213/149‑1 (MQ=255)
                                                                                      gcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCg                                                                                                                    >  1:132067/1‑92 (MQ=255)
                                                                                      gcaATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCg                                                                                                                    <  2:132067/92‑1 (MQ=255)
                                                                                                        ccGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCa                                                                                   >  3:111147/1‑107 (MQ=255)
                                                                                                        ccGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCa                                                                                   <  4:111147/107‑1 (MQ=255)
                                                                                                                          cTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcagc                         >  2:275767/1‑147 (MQ=255)
                                                                                                                           tATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcagccag                      >  1:176737/1‑149 (MQ=255)
                                                                                                                                cgcctgccgccAGGAACGCCTGGCGGCCGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATc                  >  7:14962/6‑148 (MQ=255)
                                                                                                                                acaAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGc                            <  5:55211/138‑1 (MQ=255)
                                                                                                                                acaAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGc                            >  6:55211/1‑138 (MQ=255)
                                                                                                                                acaAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATc                  >  5:294199/1‑148 (MQ=255)
                                                                                                                                    ggCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGc             <  7:260688/149‑1 (MQ=255)
                                                                                                                                    ggCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGc             <  4:240825/149‑1 (MQ=255)
                                                                                                                                       cgccAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcag                          <  3:190435/133‑1 (MQ=255)
                                                                                                                                       cgccAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGcag                          >  4:190435/1‑133 (MQ=255)
                                                                                                                                              cGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGCCAGTGCCGAg   <  3:208031/149‑1 (MQ=255)
                                                                                                                                               gTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGCCCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGCCAGTGCCGAgc  >  5:6919/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CAGTTCCAGGCCCGAGAACAGCAAGTTCAACAACTGCTCGGGCAATTGATCGACAACAGCGATGCCAGGCTCAAGGCGTCGCTGCAGCAATTGCGCGACAGCCACCGCCAGTTGGGCCAGGCCTATGCACAAGGCCGCCAGGCGTTCCTGGCGGCAGGCGGCGACCCGGTAGCGGGCGACCGGGCGGTCAAAGGCGTGGACCGTGCCGCCAGTGAGCAAATGAGCGAACTGGTCGAGCAACTGCGCGCCGATGCTCGCCAGCGTGCAGCCAGCATCAATGCCAGTGCCGAGC  >  NC_002947/2419156‑2419447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑