Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,441,353	A→G	8.7%	D164G (GAC→GGC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,441,353	0	A	G	8.7%	194.2 / 6.6	69	D164G (GAC→GGC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base G (4/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACCGACTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCG  >  NC_002947/2441206‑2441500                                                                                                                                                    |                                                                                                                                                    cAACCGACTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGAc                                                                                                                                                    <  4:252958/149‑1 (MQ=255)  aaCCGACTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACa                                                                                                                                                   >  5:24018/1‑149 (MQ=255)    ccGACTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAACCGCGTGGTGTTCAACGAAATTACCGAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATc                                                                                                                                                 >  2:14391/1‑149 (MQ=255)     cGACTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATc                                                                                                                                                 >  2:133267/1‑148 (MQ=255)       aCTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGAc                                                                                                                                              <  2:63115/149‑1 (MQ=255)            gACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGt                                                                                                                                         >  5:63782/1‑149 (MQ=255)            gACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGt                                                                                                                                         >  3:39424/1‑149 (MQ=255)                gcgAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAAt                                                                                                                                     >  7:45842/1‑149 (MQ=255)                  gAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGc                                                                                                                                   <  6:24018/149‑1 (MQ=255)                     ggggAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCca                                                                                                                                <  5:92135/149‑1 (MQ=255)                        gAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCcag                                                                                                                               >  2:58041/1‑147 (MQ=255)                             cATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGgcgc                                                                                                                        >  8:31749/1‑149 (MQ=255)                              aTTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGGCATCGACCGGGTCAATGCCCAGCAGGcgcg                                                                                                                       >  2:128286/1‑149 (MQ=255)                               ttGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCgcgg                                                                                                                      <  6:63782/149‑1 (MQ=255)                                  ccTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGt                                                                                                                   >  1:146843/1‑149 (MQ=255)                                      gCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCACGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTcc                                                                                                               <  8:53143/149‑1 (MQ=255)                                        aCCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTc                                                                                                             <  4:39424/149‑1 (MQ=255)                                         ccTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTc                                                                                                             >  5:297112/1‑148 (MQ=255)                                             cgcgAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGAcc                                                                                                         <  7:31517/148‑1 (MQ=255)                                                gAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGt                                                                                                     >  5:239593/1‑149 (MQ=255)                                                 aGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTg                                                                                                    <  3:39884/149‑1 (MQ=255)                                                   gCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGt                                                                                                  >  5:10918/1‑149 (MQ=255)                                                      aTCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCgg                                                                                               >  5:194626/1‑149 (MQ=255)                                                         gtgggTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTa                                                                                            <  6:271166/147‑1 (MQ=255)                                                             gTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATg                                                                                        <  7:31749/149‑1 (MQ=255)                                                                     acCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGcc                                                                                <  2:146843/149‑1 (MQ=255)                                                                     acCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGcc                                                                                >  8:269754/1‑149 (MQ=255)                                                                              aaGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCtgtg                                                                       >  1:275971/1‑149 (MQ=255)                                                                                gcgcGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTggg                                                                     <  6:194626/149‑1 (MQ=255)                                                                                     tggtgTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGcc                                                                   >  5:29557/1‑146 (MQ=255)                                                                                      ggtgTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGa                                                               <  7:269754/149‑1 (MQ=255)                                                                                       gtgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGGCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGa                                                               <  6:160131/148‑1 (MQ=255)                                                                                        tgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACcgc                                                                                                       <  3:7033/107‑1 (MQ=255)                                                                                        tgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACcgc                                                                                                       >  4:7033/1‑107 (MQ=255)                                                                                          ttCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTc                                                                                   >  5:162707/1‑125 (MQ=255)                                                                                          ttCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTc                                                                                   <  6:162707/125‑1 (MQ=255)                                                                                          ttCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGc                                                           >  1:26813/1‑149 (MQ=255)                                                                                             aaCGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGcc                                                                                                                                  <  8:50855/75‑1 (MQ=255)                                                                                             aaCGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGcc                                                                                                                                  >  7:50855/1‑75 (MQ=255)                                                                                             aaCGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCg                                                        >  8:5283/1‑149 (MQ=255)                                                                                             aaCGAAATTACCAAGAAGGACATTAAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGACCAGCAGCCGCGTCGTTTCCTCGAACGCTTGGTCGGTTACATGGTTTCGCAACTGCTGTGCGCCAAGATTGccct                                                        >  7:217917/1‑148 (MQ=255)                                                                                                   aTTACCGAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCt                                                  <  1:14391/149‑1 (MQ=255)                                                                                                   aTTACCAAGAATGCCATTCAGGAATCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGAGCGGCTTTTCCTCGACCGCGTGGTCGTTTAAATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCt                                                  <  8:217917/149‑1 (MQ=255)                                                                                                             agGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGGCATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTgg                                         <  1:128286/148‑1 (MQ=255)                                                                                                                  aTTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgtg                                    >  7:129983/1‑148 (MQ=255)                                                                                                                  aTTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgtg                                    >  7:218016/1‑148 (MQ=255)                                                                                                                   ttCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTa                                  <  6:29557/149‑1 (MQ=255)                                                                                                                   ttCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTa                                  <  7:5283/149‑1 (MQ=255)                                                                                                                           gCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGt                          <  8:132545/149‑1 (MQ=255)                                                                                                                           gCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGt                          <  8:128298/149‑1 (MQ=255)                                                                                                                             cTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTATGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgt                                     >  3:293777/1‑136 (MQ=255)                                                                                                                             cTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTATGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgt                                     <  4:293777/136‑1 (MQ=255)                                                                                                                              ttCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCt                                                                                                              <  5:211772/62‑1 (MQ=255)                                                                                                                              ttCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCt                                                                                                              >  6:211772/1‑62 (MQ=255)                                                                                                                                   gCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCgg                     <  2:254/146‑1 (MQ=255)                                                                                                                                         aGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCg                            <  5:181597/133‑1 (MQ=255)                                                                                                                                         aGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCg                            >  6:181597/1‑133 (MQ=255)                                                                                                                                           gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGAt                                                              <  7:23893/97‑1 (MQ=255)                                                                                                                                           gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGAt                                                              >  8:23893/1‑97 (MQ=255)                                                                                                                                           gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGACCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtggtg          <  6:239593/149‑1 (MQ=255)                                                                                                                                              tgCTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGACAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGtt       >  7:287920/2‑149 (MQ=255)                                                                                                                                              aGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGtt       <  2:26813/149‑1 (MQ=255)                                                                                                                                               gCTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTTGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtggtg          <  8:24923/145‑1 (MQ=255)                                                                                                                                               gCTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtggtg          >  7:24923/1‑145 (MQ=255)                                                                                                                                                cTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGtt       >  5:260368/1‑147 (MQ=255)                                                                                                                                                cTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTtga     <  8:129983/149‑1 (MQ=255)                                                                                                                                                cTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTATACAGTCGGTGGCGGTGAAGCTGGTGGTtga     <  2:275971/149‑1 (MQ=255)                                                                                                                                                  ggACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTtgagc   >  7:63040/1‑149 (MQ=255)                                                                                                                                                   gACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTtgagcg  >  4:308849/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAACCGACTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCG  >  NC_002947/2441206‑2441500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑