Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,441,371 C→T 7.5% A170V (GCC→GTC)  topA → DNA topoisomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,441,3710CT 7.5% 192.5 / 4.3 67A170V (GCC→GTC) topADNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (26/35);  new base T (3/2);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCGTGAGCGTGAGATCCG  >  NC_002947/2441223‑2441515
                                                                                                                                                    |                                                                                                                                                
gAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGc                                                                                                                                                  <  6:24018/149‑1 (MQ=255)
   ggggAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCca                                                                                                                                               <  5:92135/149‑1 (MQ=255)
      gAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCcag                                                                                                                                              >  2:58041/1‑147 (MQ=255)
           cATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGgcgc                                                                                                                                       >  8:31749/1‑149 (MQ=255)
            aTTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGGCATCGACCGGGTCAATGCCCAGCAGGcgcg                                                                                                                                      >  2:128286/1‑149 (MQ=255)
             ttGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCgcgg                                                                                                                                     <  6:63782/149‑1 (MQ=255)
                ccTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGt                                                                                                                                  >  1:146843/1‑149 (MQ=255)
                    gCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCACGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTcc                                                                                                                              <  8:53143/149‑1 (MQ=255)
                      aCCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTc                                                                                                                            <  4:39424/149‑1 (MQ=255)
                       ccTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTc                                                                                                                            >  5:297112/1‑148 (MQ=255)
                           cgcgAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGAcc                                                                                                                        <  7:31517/148‑1 (MQ=255)
                              gAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGt                                                                                                                    >  5:239593/1‑149 (MQ=255)
                               aGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTg                                                                                                                   <  3:39884/149‑1 (MQ=255)
                                 gCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGt                                                                                                                 >  5:10918/1‑149 (MQ=255)
                                    aTCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCgg                                                                                                              >  5:194626/1‑149 (MQ=255)
                                       gtgggTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTa                                                                                                           <  6:271166/147‑1 (MQ=255)
                                           gTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATg                                                                                                       <  7:31749/149‑1 (MQ=255)
                                                   acCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGcc                                                                                               >  8:269754/1‑149 (MQ=255)
                                                   acCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGcc                                                                                               <  2:146843/149‑1 (MQ=255)
                                                            aaGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCtgtg                                                                                      >  1:275971/1‑149 (MQ=255)
                                                              gcgcGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTggg                                                                                    <  6:194626/149‑1 (MQ=255)
                                                                   tggtgTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGcc                                                                                  >  5:29557/1‑146 (MQ=255)
                                                                    ggtgTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGa                                                                              <  7:269754/149‑1 (MQ=255)
                                                                     gtgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGGCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGa                                                                              <  6:160131/148‑1 (MQ=255)
                                                                      tgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACcgc                                                                                                                      <  3:7033/107‑1 (MQ=255)
                                                                      tgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACcgc                                                                                                                      >  4:7033/1‑107 (MQ=255)
                                                                        ttCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTc                                                                                                  <  6:162707/125‑1 (MQ=255)
                                                                        ttCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTc                                                                                                  >  5:162707/1‑125 (MQ=255)
                                                                        ttCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGc                                                                          >  1:26813/1‑149 (MQ=255)
                                                                           aaCGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGcc                                                                                                                                                 <  8:50855/75‑1 (MQ=255)
                                                                           aaCGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGcc                                                                                                                                                 >  7:50855/1‑75 (MQ=255)
                                                                           aaCGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCg                                                                       >  8:5283/1‑149 (MQ=255)
                                                                           aaCGAAATTACCAAGAAGGACATTAAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGACCAGCAGCCGCGTCGTTTCCTCGAACGCTTGGTCGGTTACATGGTTTCGCAACTGCTGTGCGCCAAGATTGccct                                                                       >  7:217917/1‑148 (MQ=255)
                                                                                 aTTACCGAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCt                                                                 <  1:14391/149‑1 (MQ=255)
                                                                                 aTTACCAAGAATGCCATTCAGGAATCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGAGCGGCTTTTCCTCGACCGCGTGGTCGTTTAAATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCt                                                                 <  8:217917/149‑1 (MQ=255)
                                                                                           agGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGGCATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTgg                                                        <  1:128286/148‑1 (MQ=255)
                                                                                                aTTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgtg                                                   >  7:218016/1‑148 (MQ=255)
                                                                                                aTTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgtg                                                   >  7:129983/1‑148 (MQ=255)
                                                                                                 ttCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTa                                                 <  6:29557/149‑1 (MQ=255)
                                                                                                 ttCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTa                                                 <  7:5283/149‑1 (MQ=255)
                                                                                                         gCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGt                                         <  8:132545/149‑1 (MQ=255)
                                                                                                         gCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGt                                         <  8:128298/149‑1 (MQ=255)
                                                                                                           cTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTATGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgt                                                    <  4:293777/136‑1 (MQ=255)
                                                                                                           cTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTATGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgt                                                    >  3:293777/1‑136 (MQ=255)
                                                                                                            ttCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCt                                                                                                                             >  6:211772/1‑62 (MQ=255)
                                                                                                            ttCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCt                                                                                                                             <  5:211772/62‑1 (MQ=255)
                                                                                                                 gCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCgg                                    <  2:254/146‑1 (MQ=255)
                                                                                                                       aGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCg                                           <  5:181597/133‑1 (MQ=255)
                                                                                                                       aGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCg                                           >  6:181597/1‑133 (MQ=255)
                                                                                                                         gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGAt                                                                             >  8:23893/1‑97 (MQ=255)
                                                                                                                         gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGAt                                                                             <  7:23893/97‑1 (MQ=255)
                                                                                                                         gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGACCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtggtg                         <  6:239593/149‑1 (MQ=255)
                                                                                                                            tgCTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGACAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGtt                      >  7:287920/2‑149 (MQ=255)
                                                                                                                            aGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGtt                      <  2:26813/149‑1 (MQ=255)
                                                                                                                             gCTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTTGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtggtg                         <  8:24923/145‑1 (MQ=255)
                                                                                                                             gCTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtggtg                         >  7:24923/1‑145 (MQ=255)
                                                                                                                              cTGGGCATTGACCCGGTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGtt                      >  5:260368/1‑147 (MQ=255)
                                                                                                                              cTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTtga                    <  8:129983/149‑1 (MQ=255)
                                                                                                                              cTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTATACAGTCGGTGGCGGTGAAGCTGGTGGTtga                    <  2:275971/149‑1 (MQ=255)
                                                                                                                                ggACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTtgagc                  >  7:63040/1‑149 (MQ=255)
                                                                                                                                 gACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTtgagcg                 >  4:308849/1‑149 (MQ=255)
                                                                                                                                       gACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTtgagcgtgagc            <  6:10918/148‑1 (MQ=255)
                                                                                                                                          ccggTCGATGTCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAgcgtgagcgtga        <  6:260368/147‑1 (MQ=255)
                                                                                                                                            ggTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCg                                                            <  2:305846/95‑1 (MQ=255)
                                                                                                                                            ggTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCg                                                            >  1:305846/1‑95 (MQ=255)
                                                                                                                                                aaTGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCGTGAGCGTGAGATCcg  <  1:58041/149‑1 (MQ=255)
                                                                                                                                                  tGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTgagcgtgagcgt          >  3:259203/1‑139 (MQ=255)
                                                                                                                                                  tGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTgagcgtgagcgt          <  4:259203/139‑1 (MQ=255)
                                                                                                                                                   gCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtg                            <  8:217721/120‑1 (MQ=255)
                                                                                                                                                   gCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCtg                            >  7:217721/1‑120 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GAAGGGGAAGCCATTGCCTGGCACCTGCGCGAGGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCGTGAGCGTGAGATCCG  >  NC_002947/2441223‑2441515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑