Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,461,715 A→C 7.6% V238V (GTA→GTC) ‡ lolC → lipoprotein releasing system transmembrane protein LolC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,461,7150AC 7.6% 154.8 / 2.9 51V238V (GTA→GTC) ‡lolClipoprotein releasing system transmembrane protein LolC
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base C (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCACGTTGGCGCGGGGGAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGC  >  NC_002947/2461574‑2461858
                                                                                                                                             |                                                                                                                                                 
cTTCCACGTTGGCGCGGGGGAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGa                                                                                                                                            <  1:171644/149‑1 (MQ=255)
 ttCCACGTTGGCGCGGGGGAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑g                                                                                                                                          >  4:271410/1‑149 (MQ=255)
   ccACGTTGGCGCGGGGGAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCTACGCGCGGCGCCTGGaa                                                                                                                                           <  2:54904/147‑2 (MQ=255)
                  ggAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTgg                                                                                                                        <  3:123208/149‑1 (MQ=255)
                       tCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCTGCTGGGCGat                                                                                                                   <  3:271410/149‑2 (MQ=255)
                       tCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGAc                                                                                                                   <  8:93220/149‑1 (MQ=255)
                            ggCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAgg                                                                                                               >  5:163300/1‑148 (MQ=255)
                            ggCTACCTGGGCCTGACCAACATAAGCGACCTGTCACGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGcc                                                                                                                                   >  2:186496/1‑129 (MQ=255)
                              cTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGACGCATCGCCCA‑GCAGCTGGGCGACCAGGaa                                                                                                             <  1:23407/149‑1 (MQ=255)
                                           aCCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGa                                                                                               >  8:67371/1‑149 (MQ=255)
                                             cAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCtata                                                                                                      >  8:191055/1‑140 (MQ=255)
                                             cAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCtata                                                                                                      <  7:191055/140‑1 (MQ=255)
                                             cAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACt                                                                                             >  3:232297/1‑149 (MQ=255)
                                               aCATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTg                                                                                            >  2:14977/1‑148 (MQ=255)
                                                       gACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTAc                                                                                   <  4:232297/149‑1 (MQ=255)
                                                         ccTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTAccc                                                                                 >  2:132101/1‑149 (MQ=255)
                                                          cTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTa                                                                                    <  8:17458/145‑1 (MQ=255)
                                                          cTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTa                                                                                    >  7:17458/1‑145 (MQ=255)
                                                           tGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAg                                                                                                                >  3:254988/1‑116 (MQ=255)
                                                           tGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAg                                                                                                                <  4:254988/116‑1 (MQ=255)
                                                             tCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAg                                                                                                     <  3:162932/125‑1 (MQ=255)
                                                             tCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAg                                                                                                     >  4:162932/1‑125 (MQ=255)
                                                             tCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACgg                                                                             >  7:20144/1‑149 (MQ=255)
                                                                   cTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCt                                                                       <  7:67371/149‑1 (MQ=255)
                                                                      cACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTa                                                                    <  6:163300/149‑1 (MQ=255)
                                                                      cACCGCTGGAAGCCAGACCAGGTGCAGGGCCTGCGCCTGAAGTTCGACGACCTTTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTa                                                                    <  5:157242/149‑1 (MQ=255)
                                                                         cGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTg                                                                      <  7:137351/144‑1 (MQ=255)
                                                                         cGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTg                                                                      >  8:137351/1‑144 (MQ=255)
                                                                         cGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCa                                                                 <  4:172436/149‑1 (MQ=255)
                                                                           cTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAgg                                                               <  1:14977/149‑1 (MQ=255)
                                                                              gAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGa                                                            >  6:212400/1‑149 (MQ=255)
                                                                               aaGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGAt                                                           <  3:48944/149‑1 (MQ=255)
                                                                                 gCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGTCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATcc                                                         >  1:241117/1‑149 (MQ=255)
                                                                                     gACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCTGTACCCACGGCAACCTGTACCAGGCGATCCGTAt                                                     <  4:74770/149‑1 (MQ=255)
                                                                                      aCCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATg                                                    >  3:298590/1‑149 (MQ=255)
                                                                                        cAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATgg                                                   >  7:268153/1‑148 (MQ=255)
                                                                                         aGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGATTTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGa                                                  >  6:316869/1‑148 (MQ=255)
                                                                                         aGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGa                                                  >  3:126724/1‑148 (MQ=255)
                                                                                          ggTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAAtt                                                                                                           <  8:89556/90‑1 (MQ=255)
                                                                                          ggTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAAtt                                                                                                           >  7:89556/1‑90 (MQ=255)
                                                                                                     tGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACAAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCACGAt                                      >  5:59549/1‑148 (MQ=255)
                                                                                                         ccTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCAGGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGc                                  >  1:82593/1‑149 (MQ=255)
                                                                                                                    acgacCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTtgctg                      <  4:126724/149‑1 (MQ=255)
                                                                                                                    acgacCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTtgctg                      <  4:298590/149‑1 (MQ=255)
                                                                                                                      gacCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTgctgct                    <  2:82593/149‑1 (MQ=255)
                                                                                                                        gatgctCCAGGCTACGCGCGGCGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGa                  >  3:110372/6‑149 (MQ=255)
                                                                                                                            ctCCAGGCTACGCGCGGCGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAAc                                                                         <  7:74453/89‑1 (MQ=38)
                                                                                                                             tCCAGGCTACGCGCGGCGCCTGGA‑GCATCGCACA‑GCAGCTGGCCGACCAGGAATTCTATAGCCGTGACTGGACCCCTACCCACGGCAACCAGTACAACGCGATACCTATGGAAAAGGCCATGATCGGCCGGCTGTTGCGGCTCAACgtt             >  6:87492/1‑148 (MQ=255)
                                                                                                                               cAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCgtgg            <  2:241117/148‑1 (MQ=255)
                                                                                                                                  gcgcCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCgtggcggt        >  2:248644/1‑149 (MQ=255)
                                                                                                                                        cgcgTAGCCTGGA‑GCATCGCCCC‑GCAGCTGGGCGACCAGGCACTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTCCCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTggcggc  <  8:268153/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CTTCCACGTTGGCGCGGGGGAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGA‑GCATCGCCCA‑GCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGC  >  NC_002947/2461574‑2461858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑