Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,480,116	G→C	6.8%	intergenic (‑55/+52)	PP_2173 ← / ← PP_2174	AraC family transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,480,116	0	G	C	6.8%	182.0 / 3.3	59	intergenic (‑55/+52)	PP_2173/PP_2174	AraC family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base C (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

TCACCGACAGGTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTATCAAGCGTTCC  >  NC_002947/2479968‑2480263                                                                                                                                                     |                                                                                                                                                     tCACCGACAGGTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCg                                                                                                                                                      >  6:21702/1‑149 (MQ=255)   aCCGACAGGTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCggg                                                                                                                                                    <  8:10699/149‑1 (MQ=255)     cGACAGGTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGt                                                                                                                                                  >  4:13299/1‑149 (MQ=255)          ggTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTa                                                                                                                                             <  5:21702/149‑1 (MQ=255)           gTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGt                                                                                                                                                  <  6:150720/143‑1 (MQ=255)           gTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGt                                                                                                                                                  >  5:150720/1‑143 (MQ=255)            tCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCCCGGTGCCtg                                                                                                                                             <  4:125750/147‑2 (MQ=255)            tCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCCCGGTGCCtg                                                                                                                                             >  3:125750/1‑146 (MQ=255)            gcACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCCCGGTGCCtgaa                                                                                                                                           <  4:95933/148‑4 (MQ=255)                cTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGt                                                                                                                                        >  7:163610/1‑148 (MQ=255)                 ttCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGTATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGa                                                                                                                                      >  2:257212/1‑149 (MQ=255)                      ggTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGct                                                                                                                                 <  3:143844/149‑1 (MQ=255)                                    tAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCtt                                                                                                                   >  7:118660/1‑149 (MQ=255)                                    tAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAAGCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCtt                                                                                                                   <  4:13427/149‑1 (MQ=255)                                       gCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGTGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGAt                                                                                                                <  1:235773/149‑1 (MQ=255)                                                     gAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGc                                                                                                    <  7:2100/147‑1 (MQ=255)                                                     gAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGc                                                                                                    >  8:2100/1‑147 (MQ=255)                                                     gAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGt                                                                                                  >  2:113290/1‑149 (MQ=255)                                                      aaCCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGtgc                                                                                                            <  3:36146/138‑1 (MQ=255)                                                      aaCCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGtgc                                                                                                            >  4:36146/1‑138 (MQ=255)                                                      aaCCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGt                                                                                                  >  6:208144/1‑148 (MQ=255)                                                      aaCCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGt                                                                                                  >  6:203484/1‑148 (MQ=255)                                                               aTGTAGGTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCTTGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCa                                                                                         <  5:221667/148‑1 (MQ=255)                                                               aTGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGCGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATATGATGCCACACGTTATGAGt                                                                                           >  6:221667/1‑146 (MQ=255)                                                                tGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAgg                                                                                       <  3:146760/149‑1 (MQ=255)                                                                 gTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGct                                                                                                                                 >  1:30653/1‑106 (MQ=255)                                                                 gTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGct                                                                                                                                 <  2:30653/106‑1 (MQ=255)                                                                  tAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAggg                                                                                      <  3:9508/148‑1 (MQ=255)                                                                        gggTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACa                                                                                 <  8:163610/147‑1 (MQ=255)                                                                        gggTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACa                                                                                 <  7:65686/147‑1 (MQ=255)                                                                        gggTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAg                                                                                <  8:118660/148‑1 (MQ=255)                                                                           ttGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTg                                                                            <  3:13299/149‑1 (MQ=255)                                                                             gtgCTGTGAGTCAGTCAACGAACGGTATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCAGTGTTCAGGCACCGGCGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGa                                                                           <  1:257212/148‑1 (MQ=255)                                                                              tgCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAg                                                                          <  5:208144/148‑1 (MQ=255)                                                                              tgCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAg                                                                          <  5:203484/148‑1 (MQ=255)                                                                                      gtcagtcaACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAgtg                                                                >  5:232182/1‑147 (MQ=255)                                                                                       tcagtcaACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCt                                                                                                                                              <  1:316684/71‑1 (MQ=255)                                                                                       tcagtcaACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCt                                                                                                                                              >  2:316684/1‑71 (MQ=255)                                                                                         agtcaACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAgggg                                                               >  8:131315/1‑147 (MQ=255)                                                                                         agtcaACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAgggg                                                               >  3:225742/1‑147 (MQ=255)                                                                                               cgaacgGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCg                                                       >  8:217268/1‑149 (MQ=255)                                                                                                gaacgGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGt                                                      >  2:86346/1‑149 (MQ=255)                                                                                                 aacgGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGa                                                                           >  7:239212/1‑128 (MQ=255)                                                                                                 aacgGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGa                                                                           <  8:239212/128‑1 (MQ=255)                                                                                                       aTCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACa                                                                                 >  6:166899/1‑116 (MQ=255)                                                                                                       aTCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACa                                                                                 <  5:166899/116‑1 (MQ=255)                                                                                                              gAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGt                                        <  7:131315/149‑1 (MQ=255)                                                                                                                                   aTCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGaa                     >  5:225004/1‑147 (MQ=255)                                                                                                                                    tCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCCTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACATGGTGACTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAg                  <  1:190900/149‑1 (MQ=255)                                                                                                                                      cGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGa                <  7:217268/149‑1 (MQ=255)                                                                                                                                      cGTTGTGCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGAGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGa                <  7:126147/149‑1 (MQ=255)                                                                                                                                        ttgttCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGt              <  4:225742/149‑1 (MQ=255)                                                                                                                                          gttCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTAt            <  1:113290/149‑1 (MQ=255)                                                                                                                                           ttCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACCTAAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTAt            <  1:86346/149‑1 (MQ=255)                                                                                                                                            ctaGGCACCCCGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTATc           >  8:3820/3‑148 (MQ=255)                                                                                                                                                gCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGAc                                                  <  2:43121/105‑1 (MQ=255)                                                                                                                                                gCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGAc                                                  >  1:43121/1‑105 (MQ=255)                                                                                                                                                gCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTATCAAGCg      >  6:91079/1‑149 (MQ=255)                                                                                                                                                  aCCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTATCAAGCGtt    >  8:254376/1‑149 (MQ=255)                                                                                                                                                  aCCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCGGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTATCAAGCGtt    >  1:229730/1‑149 (MQ=255)                                                                                                                                                    cGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTATCAAGCGTTcc  <  6:225004/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TCACCGACAGGTCACCTTCGAGGTGGCTGTCGATGTAGGCAAGCACGGTTTCGAACCGTTTCATGTAGCTAGGGTTGTGCTGTGAGTCAGTCAACGAACGGAATCTCTGGAAGGTGCGGTAAGCGCTAGCATCCGTTGTTCAGGCACCGGGGTGCCTAGCCGTGATTGCTCTATCGGGTGGCTTGATGTGCTGCCAAGCGTTATGAGTCAGGGTTACAGGGTGAGTACC‑AAGGGGCAGCAGCGTCGACGTGAACATGTTTCTGCTTCCTGGGCAGAAAAGGAGTATCAAGCGTTCC  >  NC_002947/2479968‑2480263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑