Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,495,416 C→T 8.0% intergenic (+7/+16) PP_2188 → / ← PP_2189 tRNA 2‑methylthio‑N6‑isopentenyl adenosine(37) hydroxylase MiaE/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,495,4160CT 8.0% 143.3 / 2.4 50intergenic (+7/+16)PP_2188/PP_2189tRNA 2‑methylthio‑N6‑isopentenyl adenosine(37) hydroxylase MiaE/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/20);  new base T (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

TGGCGCACAACTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTT  >  NC_002947/2495273‑2495563
                                                                                                                                                |                                                                                                                                                   
tGGCGCACAACTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTAGGCCGCCTGATGGGCACCAggc                                                                                                                                                >  6:167334/1‑149 (MQ=255)
   cgcACAACTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAggcggc                                                                                                                                             <  4:21699/149‑1 (MQ=255)
     cacaACTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGGTGCCCATCAggcggcca                                                                                                                                           <  6:141409/149‑3 (MQ=255)
      acaACTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGgcggcg                                                                                                                                            >  7:178953/1‑147 (MQ=255)
       ctaCTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGGACGCGCGGCGGAAATGGAGCTG‑ATCCAGGCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCGCGCAGGCTTTTGCCGCCTGATGGGCACCAGGCGGCGCAg                                                                                                                                         <  5:167334/147‑1 (MQ=255)
          cTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGTCCGCCTGATGGGCACCAGGCGGCGCAGGcc                                                                                                                                      <  2:41645/149‑1 (MQ=255)
           tACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCt                                                                                                                                     >  1:167597/1‑149 (MQ=255)
                    gaAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGGTGCCCATCAggcggccaaag                                                                                                                                        >  8:223566/1‑132 (MQ=255)
                    gaAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGGTGCCCATCAggcggccaaag                                                                                                                                        <  7:223566/137‑6 (MQ=255)
                       gCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCgg                                                                                                                         >  1:110118/1‑149 (MQ=255)
                             aTCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTGCATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAg                                                                                                                    >  1:224725/1‑149 (MQ=255)
                                     ctGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGcc                                                                                                           >  7:263458/1‑149 (MQ=255)
                                            gAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGc                                                                                                     >  8:93827/1‑148 (MQ=255)
                                                  gTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGccc                                                                                               >  4:10099/1‑148 (MQ=255)
                                                   tACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGa                                                                                             <  7:126661/149‑1 (MQ=255)
                                                       cgcgGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGcc                                                                                         <  8:178953/149‑1 (MQ=255)
                                                        gcggcgGAAATGGAGCTG‑ATCCAGTCACCAGACAAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGAGCCGGCCCAGTAGACGGCCTACCGCCACCCCCAGGCCg                                                                                        >  8:126938/1‑149 (MQ=255)
                                                            cggAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCGTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAg                                                                                    <  4:15366/149‑1 (MQ=255)
                                                                  tGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAg                                                                              <  2:164557/149‑1 (MQ=255)
                                                                     aGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCaa                                                                           <  7:93827/149‑1 (MQ=255)
                                                                      gCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCaa                                                                           >  7:160673/1‑148 (MQ=255)
                                                                         g‑ATCCAGTCACCAGACCAGGAGCTGCGCTTACACAGCGGCATACCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAg                                                                                                                    >  1:140628/1‑104 (MQ=255)
                                                                          gatggaGTCACGAGAGCAGGAGCTGCGCTTCCACAGCTGCATGCCGCAGGGTTTGGCCGCCTGATGGTCACCAGGCGGCGCAGGGCTTAACTGCGCCGTCCCAg                                                                                                                    <  2:140628/99‑1 (MQ=255)
                                                                             ccAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTg                                                                    >  4:168077/1‑149 (MQ=255)
                                                                               aGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCATTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGAAACAGTCTGc                                                                   >  2:87555/1‑148 (MQ=255)
                                                                               aGTCACCAGACCAGGAGCTGCGATTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGcc                                                                  >  8:15899/1‑149 (MQ=255)
                                                                                gTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGc                                                                   >  2:291723/1‑147 (MQ=255)
                                                                                   accacaccacGCGCCGCGCCGCCACAGCGGCATCCCCCAGGCTTTGGCCGCCTGATGGGCACCACGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAggg                                                              <  4:286696/149‑1 (MQ=255)
                                                                                    ccagaccagGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAgggg                                                             <  8:165630/149‑1 (MQ=255)
                                                                                      agaccagGAGCTGCGCTTCCTCAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTg                                                           <  7:126938/149‑1 (MQ=255)
                                                                                      agaccagGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTg                                                           >  3:236346/1‑149 (MQ=255)
                                                                                      agaccagGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTg                                                           >  6:40963/1‑149 (MQ=255)
                                                                                                cTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGTCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGACGCCGCCAGAAATAGCAACAGACTGCCAGCGCTGgcacatcgg                                                  >  6:74673/1‑141 (MQ=255)
                                                                                                   cgcTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTa                                               <  2:167597/148‑1 (MQ=255)
                                                                                                      ttCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTcc                                           >  6:191426/1‑149 (MQ=255)
                                                                                                      ttCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTcc                                           >  6:44498/1‑149 (MQ=255)
                                                                                                        ccACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGt                                                <  2:136547/142‑1 (MQ=255)
                                                                                                        ccACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGt                                                >  1:136547/1‑142 (MQ=255)
                                                                                                         cacaGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTgg                                        <  4:68767/149‑1 (MQ=255)
                                                                                                         cacaGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTgg                                        >  3:68767/1‑149 (MQ=255)
                                                                                                            aGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTg                                     <  3:10099/149‑1 (MQ=255)
                                                                                                              cGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGc                                   >  1:146697/1‑149 (MQ=255)
                                                                                                              cGGCATCCCGCAGGCTTTGGCCGCCTGAGGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGc                                   <  2:115338/149‑1 (MQ=255)
                                                                                                                gCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGAAGTAGTCCTGGGAGGcct                                 >  7:239486/1‑147 (MQ=255)
                                                                                                                         aGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGTCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCg                        >  2:67624/1‑149 (MQ=255)
                                                                                                                             tttGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTgg                                        >  6:313395/1‑129 (MQ=255)
                                                                                                                             tttGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTgg                                        <  5:313395/129‑1 (MQ=255)
                                                                                                                             ctgcgccgccTGGTGCCCATCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTg                    >  2:6533/5‑149 (MQ=255)
                                                                                                                                      cTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTtgct           <  8:160673/149‑1 (MQ=255)
                                                                                                                                            atcgccAGGCGGCGCAGGCCTTACCTGCGCCGGCCCAGGAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAACTAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCGGGGGGGCGTCCACCACCGGTTGCGCCTTGCTGCGCAc     <  8:155955/145‑1 (MQ=255)
                                                                                                                                               aCCAGGCGGCGCAGGCCTTAACTGCGCCCGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGtt  <  5:191426/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGGCGCACAACTACGGTGATGAAGCGGACATCGCTCGCTGCGTCGAGCTGGTACGCGCGGCGGAAATGGAGCTG‑ATCCAGTCACCAGACCAGGAGCTGCGCTTCCACAGCGGCATCCCGCAGGCTTTGGCCGCCTGATGGGCACCAGGCGGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTT  >  NC_002947/2495273‑2495563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑