| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,542,853 | C→A | 14.8% | L105M (CTG→ATG) ‡ | PP_2235 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,542,853 | 0 | C | A | 14.8% | 67.1 / 5.3 | 27 | L105M (CTG→ATG) ‡ | PP_2235 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (11/12); new base A (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
TTGCAGTACCTGCAATGGCAGTCACGCCAGGCCTGGGCTGCGGCGGCCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGGAGCGAGCTATACGCTTTCTGGC > NC_002947/2542715‑2543000 | ttGCAGTACCTGCAATGGCAGTCACGCCAGGCCTGGGCTGCGGCGGCCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCgg < 3:106628/149‑1 (MQ=255) tGGCAGTCACGCCAGGCCTGGGCTGCGGCGGCCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGtgct > 7:154692/1‑149 (MQ=255) ccAGGCCTGGGCTGCGGCGGCCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGa < 3:2304/149‑1 (MQ=255) tGCGGCGGCCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCCGCGCACCAGGCTGCGCagggc < 4:39722/114‑2 (MQ=255) tGCGGCGGCCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCCGCGCACCAGGCTGCGCagggc > 3:39722/1‑113 (MQ=255) ggcggcCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCAtt > 4:20624/1‑128 (MQ=255) ggcggcCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCAtt < 3:20624/128‑1 (MQ=255) gcggcCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGTGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAgg > 4:102253/1‑148 (MQ=255) ggcCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCAt < 8:154692/149‑1 (MQ=255) ttCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGc > 1:305210/1‑149 (MQ=255) gTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAg < 2:305210/148‑1 (MQ=255) tttCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGAt < 2:79916/107‑1 (MQ=255) tttCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGAt > 1:79916/1‑107 (MQ=255) tttCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCCGCGCACCAGGCTGCGCaggg < 5:315719/82‑1 (MQ=37) tttCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCCGCGCACCAGGCTGCGCaggg > 6:315719/1‑82 (MQ=37) gACCTGCTGGGCCAGCATCAGGCAGGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAg > 5:98729/1‑149 (MQ=255) ctgctgGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCg > 6:118534/1‑149 (MQ=255) aCGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGc < 3:102253/149‑1 (MQ=255) aCTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATgcc > 4:89283/1‑149 (MQ=255) cTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATgccg > 3:139960/1‑149 (MQ=255) gccgccTACAAGGCCCTGCGCAGCCTGGAGCGCGGGGACGATCGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAg > 7:230507/1‑149 (MQ=255) ccTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGgagc < 4:139960/149‑1 (MQ=255) ccAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGgagcga < 8:230507/147‑1 (MQ=255) agCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGGAGCGAGCtat < 5:18721/148‑1 (MQ=255) cTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGGAGCGAGCTATACGc > 1:153202/1‑149 (MQ=255) cgcAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGGAGCGAGCTATACGCt < 5:118534/147‑1 (MQ=255) gCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGGAGCGAGCTATACGCTTTCTgg < 3:89283/149‑1 (MQ=255) ccTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGGAGCGAGCTATACGCTTTCTGGc > 7:125094/1‑149 (MQ=255) | TTGCAGTACCTGCAATGGCAGTCACGCCAGGCCTGGGCTGCGGCGGCCGTGCACTTCGTCGAGGAATCGTTTCTTGACCTGCTGGGCCAGCATCAGGCACGCGGTGTCAACTTCGCCGCCTACCAGGCCCTGCGCAGCCTGGTGCGCGGGGACGATGGCGTGCTGCATTGCCAGATGGGCGGTCCTCAGGCATACCAAGGTGCGTAGTGCGGGTAGCGATCCAGGCGGGCACCGATGACCATTTCGCTGATCCATGCCGCCAGGAGCGAGCTATACGCTTTCTGGC > NC_002947/2542715‑2543000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |