Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,568,701 C→A 8.9% V283F (GTC→TTC)  PP_2252 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,568,7010CA 8.9% 118.4 / 2.3 42V283F (GTC→TTC) PP_2252hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/16);  new base A (2/2);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

CCGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGC  >  NC_002947/2568554‑2568848
                                                                                                                                                    |                                                                                                                                                   
cgggCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACATGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGTTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGAcc                                                                                                                                                    <  7:273976/147‑1 (MQ=255)
 cGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCa                                                                                                                                                   >  7:16396/1‑149 (MQ=255)
  ggCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGATGGTCGAACaa                                                                                                                                                  >  3:48209/1‑148 (MQ=255)
        ggcgCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                            <  4:48209/149‑1 (MQ=255)
               tGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCa                                                                                                                                     >  6:177478/1‑149 (MQ=255)
                 gCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCa                                                                                                                                     <  7:277321/147‑1 (MQ=255)
                        aGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATgg                                                                                                                            <  5:177478/149‑1 (MQ=255)
                               cTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTGCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtcatca                                                                                                                     >  6:291221/1‑149 (MQ=255)
                                tCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCAc                                                                                                                    >  2:312144/1‑149 (MQ=255)
                                tCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCAc                                                                                                                    >  2:5459/1‑149 (MQ=255)
                                tCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                            <  3:182299/125‑1 (MQ=255)
                                tCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                            >  4:182299/1‑125 (MQ=255)
                                        ccccGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAAc                                                                                                            <  3:127374/149‑1 (MQ=255)
                                               aGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGc                                                                                                     <  3:285412/149‑1 (MQ=255)
                                                ggTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCg                                                                                                    <  8:16396/149‑1 (MQ=255)
                                                 gTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCgg                                                                                                   >  2:211796/1‑149 (MQ=255)
                                                          ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGGTTAAGGATCACCCGAAGTTGTTCGCCCAACTGCGGTTGGGCACGGGCATGGTGCATCCCCCCTTACCCGGGTGCGCCatacga                                                                                            >  1:90251/1‑143 (MQ=255)
                                                                  tGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                   >  1:15870/1‑100 (MQ=255)
                                                                  tGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                   <  2:15870/100‑1 (MQ=255)
                                                                  tGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGcc                                                                                  >  8:116482/1‑149 (MQ=255)
                                                                     ccccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTCGTTCAACGCCAC‑GTTCAGGATCACCCGCAGATGTTCGACCATCTGCGGGTGGTCACAGTCATGGGTCATCACCCCGAAACCGGCTGAGGCAATCGAAATCATCGCCCCt                                                                               >  4:20312/1‑149 (MQ=255)
                                                                           tctGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCAcc                                                                         >  5:119476/1‑149 (MQ=255)
                                                                                        cATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGcc                                                             >  2:85002/1‑148 (MQ=255)
                                                                                          tCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGAt                                                          <  1:211796/149‑1 (MQ=255)
                                                                                                    ccATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGc                                                  <  1:85002/147‑1 (MQ=255)
                                                                                                       ttacTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCATGCAACCTTGCCCACTGCCGAt                                                          >  3:54933/4‑136 (MQ=255)
                                                                                                       ttCCTGGTTCAACGCCAC‑GTTCAGGATCACCCTCAGTTGTTCGAGCATCTGCGGTTGGTCACGGTCATTGGTCATCACCCCGTAACCTGATGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCGGAt                                                          <  4:54933/136‑1 (MQ=255)
                                                                                                         ccTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCACTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGc                                            >  7:62429/1‑148 (MQ=255)
                                                                                                          cTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGa                                          >  1:169558/1‑149 (MQ=255)
                                                                                                           tGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGc                                                                             <  6:235877/113‑1 (MQ=255)
                                                                                                              ttCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATccc                                      <  7:116482/149‑1 (MQ=255)
                                                                                                                    gCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTgg                                <  2:169558/149‑1 (MQ=255)
                                                                                                                          gTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGc                             >  7:115397/1‑147 (MQ=255)
                                                                                                                            tCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTCCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAgg                         >  7:125638/1‑149 (MQ=255)
                                                                                                                             cAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGGGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAgg                         <  2:90251/148‑1 (MQ=255)
                                                                                                                                gATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTgg                     >  6:302046/1‑149 (MQ=255)
                                                                                                                                      ccGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAatcatc                                                                                     >  6:205846/1‑79 (MQ=255)
                                                                                                                                      ccGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAatcatc                                                                                     <  5:205846/79‑1 (MQ=255)
                                                                                                                                          aGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCtt           >  4:94881/1‑149 (MQ=255)
                                                                                                                                                  gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCcg                                     <  7:50930/115‑1 (MQ=255)
                                                                                                                                                  gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCcg                                     >  8:50930/1‑115 (MQ=255)
                                                                                                                                                  gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTg    <  1:312144/148‑1 (MQ=255)
                                                                                                                                                   aCCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGc  <  5:291221/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCAC‑GTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGC  >  NC_002947/2568554‑2568848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑