Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 318,967 G→A 10.7% R259C (CGC→TGC)  PP_0264 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947318,9670GA10.7% 150.4 / 7.2 56R259C (CGC→TGC) PP_0264sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (2/4);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

GCCGCCGTTGCATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGAT  >  NC_002947/318823‑319114
                                                                                                                                                  |                                                                                                                                                   
gccgccGTTGCATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTc                                                                                                                                                 >  5:219236/1‑149 (MQ=255)
    ccgTTGCATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCAC‑‑ACCAGTTTCCTTGATGTCGCGGGTCTggg                                                                                                                                             >  3:182932/1‑149 (MQ=255)
       ttGCATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGa                                                                                                                                          <  5:157351/149‑1 (MQ=255)
       ttGCATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCAGGCCTggatca                                                                                                                                          >  7:144267/1‑145 (MQ=255)
         gCATCACCAGGCCTGCCAGTAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATc                                                                                                                                        >  4:86016/1‑149 (MQ=255)
          cATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATc                                                                                                                                        >  7:242247/1‑148 (MQ=255)
          cATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATc                                                                                                                                        >  4:242293/1‑148 (MQ=255)
          cATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGAATTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATc                                                                                                                                        >  2:91004/1‑148 (MQ=255)
           aTCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCAGGCCTggatcacc                                                                                                                                        >  2:175769/1‑141 (MQ=255)
             cACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAgg                                                                                                                                    >  7:251060/1‑149 (MQ=255)
             cACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAgg                                                                                                                                    >  3:56169/1‑149 (MQ=255)
               ccAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGcc                                                                                                                                  <  8:251060/149‑1 (MQ=255)
                 aGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCAGGCCTGGATCACCCAGAcccg                                                                                                                                <  1:175769/149‑3 (MQ=255)
                 aGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCAGGCCTGGATCACCCAGAcccg                                                                                                                                <  8:144267/149‑3 (MQ=255)
                  ggCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGc                                                                                                                               <  4:56169/149‑1 (MQ=255)
                  ggCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCAGGCCTGGATCACCCAGACCCGc                                                                                                                               <  7:256840/149‑1 (MQ=255)
                   gCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCg                                                                                                                              >  8:213613/1‑149 (MQ=255)
                     cTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTgg                                                                                                                                              <  4:37448/131‑1 (MQ=255)
                     cTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTgg                                                                                                                                              >  3:37448/1‑131 (MQ=255)
                        ccAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTg                                                                                                                         <  1:93101/149‑1 (MQ=255)
                        ccAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCAGGCCTGGATCACCCAGACCcgcgacat                                                                                                                          <  7:30462/148‑5 (MQ=255)
                         cAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGTACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTga                                                                                                                        <  2:311479/149‑1 (MQ=255)
                         cAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTga                                                                                                                        <  8:242247/149‑1 (MQ=255)
                                   aGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCg                                                                                                               <  3:242293/148‑1 (MQ=255)
                                    gTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGATTTGCCACCACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCg                                                                                                               <  1:91004/149‑1 (MQ=255)
                                      aCCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCtg                                                                                                           >  4:88804/1‑149 (MQ=255)
                                       ccAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCtgt                                                                                                          <  8:244316/149‑1 (MQ=255)
                                         agcagcGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCgg                                                                                                                             <  2:27079/128‑1 (MQ=255)
                                         agcagcGTACCGGCACCGATGGCCATTACCCTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCgg                                                                                                                             >  1:27079/1‑128 (MQ=255)
                                          gcagcGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTggg                                                                                                       <  3:305826/149‑1 (MQ=255)
                                          gcagcGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTggg                                                                                                       >  6:63024/1‑149 (MQ=255)
                                                     gCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCg                                                                                             >  3:309502/1‑148 (MQ=255)
                                                       aCCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGAt                                                                                          <  3:86016/149‑1 (MQ=255)
                                                         cGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTc                                                                                              <  1:89215/143‑1 (MQ=255)
                                                         cGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTc                                                                                              >  2:89215/1‑143 (MQ=255)
                                                              gCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTg                                                                                   <  8:146743/149‑1 (MQ=255)
                                                                          tGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCggg                                                                                                                            >  7:58240/1‑96 (MQ=255)
                                                                          tGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCggg                                                                                                                            <  8:58240/96‑1 (MQ=255)
                                                                            cACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATgg                                                                     >  8:136918/1‑149 (MQ=255)
                                                                                gAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTg                                                                 <  5:63024/149‑1 (MQ=255)
                                                                                 aGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTgg                                                                >  2:161553/1‑149 (MQ=255)
                                                                                           cAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGt                                                      >  4:246265/1‑149 (MQ=255)
                                                                                           cAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGt                                                      <  7:136918/149‑1 (MQ=255)
                                                                                                                gCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCAc                                 >  2:209570/1‑149 (MQ=255)
                                                                                                                  tGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACg                                >  3:126553/1‑148 (MQ=255)
                                                                                                                      ttGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTGTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAgc                           >  1:25328/1‑149 (MQ=255)
                                                                                                                         ccA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAgcgc                         <  1:209570/148‑1 (MQ=255)
                                                                                                                         ccA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAgcgc                         <  2:25328/148‑1 (MQ=255)
                                                                                                                               ccAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGaa                             <  6:312936/140‑1 (MQ=255)
                                                                                                                               ccAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGaa                             >  5:312936/1‑140 (MQ=255)
                                                                                                                                      ccTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCgat                                                                          >  4:256341/1‑88 (MQ=255)
                                                                                                                                      ccTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCgat                                                                          <  3:256341/88‑1 (MQ=255)
                                                                                                                                       cTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCcgc             >  6:325735/1‑148 (MQ=255)
                                                                                                                                        ttGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCcgcg            >  6:105319/1‑148 (MQ=255)
                                                                                                                                          taTGTCGCGGGGCGGGGTGATCCAGGCCGGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGaa          <  4:182932/147‑1 (MQ=255)
                                                                                                                                           aTGGCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGt        <  7:213613/149‑1 (MQ=255)
                                                                                                                                                gcgGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAgg    <  3:88804/148‑1 (MQ=255)
                                                                                                                                                 cgGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGAt  <  4:126553/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCCGCCGTTGCATCACCAGGCCTGCCAGCAACATCAGTACCAGCAGCGTACCGGCACCGATGGCCATTACCGTCTGCACCGAGCGATCAACCAGCGTGCGCGGGGCGAGGATGCTGACTTGCCA‑‑ACCAGTTTCCTTGATGTCGCGGGTCTGGGTGATCCAGGCCTGCGGGTTGAGAACCAGCGGCTGTGGGGCCTGGGTCGGATAGGGCTGGATGGCGATGATGGCCTGGCGCTCGGCGTCGGTCAGCGTGCGGGTAGCACGGAAGCGCCAGTCGGGCCGCGAAGTCAGGAT  >  NC_002947/318823‑319114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑