Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,603,077	A→C	13.1%	K12Q (AAG→CAG) ‡	PP_5513 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,603,077	0	A	C	13.1%	162.8 / 15.5	61	K12Q (AAG→CAG) ‡	PP_5513	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/30);  new base C (3/5);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

AGGCGATGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCAC  >  NC_002947/2602931‑2603224                                                                                                                                                       |                                                                                                                                                    aGGCGATGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCaa                                                                                                                                                    <  4:9651/148‑1 (MQ=255) aGGCGATGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCaa                                                                                                                                                    <  2:167466/148‑1 (MQ=255) aGGCGATGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAg                                                                                                                                                   >  5:61381/1‑149 (MQ=255)  ggCGATGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGt                                                                                                                                                  <  3:221699/149‑1 (MQ=255)  ggCGATGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGt                                                                                                                                                  <  1:34639/149‑1 (MQ=255)       tGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCCACttgatg                                                                                                                                             <  7:229157/149‑1 (MQ=255)       tGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGtggat                                                                                                                                              <  5:21662/148‑1 (MQ=255)          gCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGtggatgga                                                                                                                                           <  4:9376/148‑1 (MQ=255)          gCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATggag                                                                                                                                          <  5:156123/149‑1 (MQ=255)                 gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTTCCTCCATCCACTTGATGGA‑GAAGTgt                                                                                                                                   <  4:295297/149‑2 (MQ=255)                 gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTg                                                                                                                                    >  2:45613/1‑148 (MQ=255)                 gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑AA‑‑ACACTT‑CTCCATCAAGtggatg                                                                                                                                             <  8:40577/139‑1 (MQ=255)                 gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑AA‑‑ACACTT‑CTCCATCAAGtggatg                                                                                                                                             >  7:40577/1‑139 (MQ=255)                     tACCGCAGTGACCCGGCATAACGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATGAAGTGGATGGAGGAAGTGGATgg                                                                                                                               >  4:54787/1‑149 (MQ=255)                       ccGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTTCCTCCATCCACTTGATGGA‑GAAGTgttcgtc                                                                                                                              <  3:238849/148‑7 (MQ=38)                       ccGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTTCCTCCATCCACTTGATGGA‑GAAGTgttcgtc                                                                                                                              <  5:62933/148‑7 (MQ=38)                        cGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCTATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGt                                                                                                                            <  2:63743/149‑1 (MQ=255)                            gTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGac                                                                                                                        <  2:187409/149‑1 (MQ=255)                                cccGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGactact                                                                                                                    <  6:61381/149‑1 (MQ=255)                                cccGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGactact                                                                                                                    <  1:221379/149‑1 (MQ=255)                                  cGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCCACTTGATggagaa                                                                                                                                        >  2:100096/1‑125 (MQ=255)                                  cGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCCACTTGATggagaa                                                                                                                                        <  1:100096/127‑3 (MQ=255)                                        aCCGTGCCCTAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAATACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGa                                                                                                                         <  6:181377/136‑1 (MQ=255)                                        aCCGTGCCAGAGTCCAGGCCAAGCTCCTCCGTTCCAAAGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGCCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGa                                                                                                                         >  5:181377/1‑136 (MQ=255)                                           gTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCg                                                                                                         >  3:106133/1‑149 (MQ=255)                                              cccGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCAACAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCt                                                                                                      >  3:200119/1‑149 (MQ=255)                                                      cAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGACTTACTACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCg                                                                                                 <  1:256759/149‑1 (MQ=255)                                                       aGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTAc                                                                                             >  4:111883/1‑149 (MQ=255)                                                        ggCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACt                                                                                            <  7:79504/149‑1 (MQ=255)                                                         gCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTg                                                                                           <  6:272337/149‑1 (MQ=255)                                                               ctcctcCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCgg                                                                                     <  8:93294/149‑1 (MQ=255)                                                                     cGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCg                                                                                <  6:180384/148‑1 (MQ=255)                                                                       ttCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAg                                                                             >  6:82438/1‑149 (MQ=255)                                                                          cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCg                                                                                                                             <  5:83065/98‑1 (MQ=255)                                                                          cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCg                                                                                                                             >  6:83065/1‑98 (MQ=255)                                                                          cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGa                                                                          <  4:200119/149‑1 (MQ=255)                                                                          cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGa                                                                          >  3:110385/1‑149 (MQ=255)                                                                           aaCGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAA‑GA‑‑ACACTT‑CTCCTTCAAGTGGATGGAGGTAGTTGATGGAGTGGACTACTGGGCATCTTCGGCTTTCCGCTACTGGATCGGTCCCGAAGGGAc                                                                         <  7:194718/149‑1 (MQ=255)                                                                               tcttctGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTggg                                                                                                                 <  1:121957/105‑1 (MQ=255)                                                                               tcttctGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTggg                                                                                                                 >  2:121957/1‑105 (MQ=255)                                                                                    tGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTc                                                                                                          <  5:309811/107‑1 (MQ=255)                                                                                    tGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTc                                                                                                          >  6:309811/1‑107 (MQ=255)                                                                                            ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg                                                                                                                                   <  4:16127/74‑1 (MQ=255)                                                                                            ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg                                                                                                                                   <  4:11930/74‑1 (MQ=255)                                                                                            ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg                                                                                                                                   >  3:16127/1‑74 (MQ=255)                                                                                            ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg                                                                                                                                   >  3:11930/1‑74 (MQ=255)                                                                                             gCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGAt                                                         <  5:82438/147‑1 (MQ=255)                                                                                             gCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGAt                                                         <  3:111883/147‑1 (MQ=255)                                                                                               cGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTcc                                                     >  1:125266/1‑149 (MQ=255)                                                                                                gCAATAAGAAAAACAACAATGAGCGGCTGCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTAGCCTTTCCGCTACTGGATAGGTCCCGAAGGGACTGGCCGGTACGTGGGTGTccc                                                    >  1:18845/1‑149 (MQ=255)                                                                                                  aaTAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCg                                                  >  4:143262/1‑149 (MQ=255)                                                                                                       gAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGt                                                             <  6:15799/133‑1 (MQ=255)                                                                                                       gAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGt                                                             >  5:15799/1‑133 (MQ=255)                                                                                                                  aTGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGa                                  <  1:45613/149‑1 (MQ=255)                                                                                                                      gCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATg                                <  2:15502/147‑1 (MQ=255)                                                                                                                      gCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATg                                >  1:15502/1‑147 (MQ=255)                                                                                                                              aCCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGt                      <  4:110385/149‑1 (MQ=255)                                                                                                                                  ac‑ga‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGGTGGAGCTTCTGTAccc                  >  6:224705/1‑149 (MQ=255)                                                                                                                                         acacTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCccgtc              >  3:58944/1‑149 (MQ=255)                                                                                                                                           acttcctcCATCCACTTGATGGA‑GAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTCCCccgtccg            >  7:54639/6‑149 (MQ=255)                                                                                                                                           acttcctcCATCCACTTGATGGA‑GAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACAccgtccg            >  7:58995/6‑149 (MQ=255)                                                                                                                                                   cATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTgg      <  3:143262/148‑1 (MQ=255)                                                                                                                                                   cATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGt     >  2:264451/1‑149 (MQ=255)                                                                                                                                                       aaGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGGTGGAGCTTCTGTACCCCGTCCGTTCTGGTCAc  <  5:224705/148‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                    AGGCGATGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCAC  >  NC_002947/2602931‑2603224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑