| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,603,081 | G→T | 12.8% | W13L (TGG→TTG) | PP_5513 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,603,081 | 0 | G | T | 12.8% | 155.1 / 11.1 | 54 | W13L (TGG→TTG) | PP_5513 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (21/26); new base T (3/4); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
TGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCAC > NC_002947/2602937‑2603224 | tGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCCACttgatg < 7:229157/149‑1 (MQ=255)tGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGtggat < 5:21662/148‑1 (MQ=255) gCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGtggatgga < 4:9376/148‑1 (MQ=255) gCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATggag < 5:156123/149‑1 (MQ=255) gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTTCCTCCATCCACTTGATGGA‑GAAGTgt < 4:295297/149‑2 (MQ=255) gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTg > 2:45613/1‑148 (MQ=255) gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑AA‑‑ACACTT‑CTCCATCAAGtggatg > 7:40577/1‑139 (MQ=255) gcgcTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑AA‑‑ACACTT‑CTCCATCAAGtggatg < 8:40577/139‑1 (MQ=255) tACCGCAGTGACCCGGCATAACGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATGAAGTGGATGGAGGAAGTGGATgg > 4:54787/1‑149 (MQ=255) ccGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTTCCTCCATCCACTTGATGGA‑GAAGTgttcgtc < 3:238849/148‑7 (MQ=38) ccGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTTCCTCCATCCACTTGATGGA‑GAAGTgttcgtc < 5:62933/148‑7 (MQ=38) cGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCTATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGt < 2:63743/149‑1 (MQ=255) gTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGac < 2:187409/149‑1 (MQ=255) cccGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGactact < 1:221379/149‑1 (MQ=255) cccGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGactact < 6:61381/149‑1 (MQ=255) cGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCCACTTGATggagaa > 2:100096/1‑125 (MQ=255) cGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCCACTTGATggagaa < 1:100096/127‑3 (MQ=255) aCCGTGCCCTAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAATACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGa < 6:181377/136‑1 (MQ=255) aCCGTGCCAGAGTCCAGGCCAAGCTCCTCCGTTCCAAAGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGCCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGa > 5:181377/1‑136 (MQ=255) gTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCg > 3:106133/1‑149 (MQ=255) cccGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCAACAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCt > 3:200119/1‑149 (MQ=255) cAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGACTTACTACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCg < 1:256759/149‑1 (MQ=255) aGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTAc > 4:111883/1‑149 (MQ=255) ggCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACt < 7:79504/149‑1 (MQ=255) gCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTg < 6:272337/149‑1 (MQ=255) ctcctcCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCgg < 8:93294/149‑1 (MQ=255) cGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCg < 6:180384/148‑1 (MQ=255) ttCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAg > 6:82438/1‑149 (MQ=255) cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCg < 5:83065/98‑1 (MQ=255) cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCg > 6:83065/1‑98 (MQ=255) cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGa < 4:200119/149‑1 (MQ=255) cAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGa > 3:110385/1‑149 (MQ=255) aaCGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAA‑GA‑‑ACACTT‑CTCCTTCAAGTGGATGGAGGTAGTTGATGGAGTGGACTACTGGGCATCTTCGGCTTTCCGCTACTGGATCGGTCCCGAAGGGAc < 7:194718/149‑1 (MQ=255) tcttctGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTggg < 1:121957/105‑1 (MQ=255) tcttctGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTggg > 2:121957/1‑105 (MQ=255) tGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTc < 5:309811/107‑1 (MQ=255) tGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTc > 6:309811/1‑107 (MQ=255) ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg < 4:16127/74‑1 (MQ=255) ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg < 4:11930/74‑1 (MQ=255) ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg > 3:16127/1‑74 (MQ=255) ggCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTgg > 3:11930/1‑74 (MQ=255) gCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGAt < 5:82438/147‑1 (MQ=255) gCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGAt < 3:111883/147‑1 (MQ=255) cGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTcc > 1:125266/1‑149 (MQ=255) gCAATAAGAAAAACAACAATGAGCGGCTGCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTAGCCTTTCCGCTACTGGATAGGTCCCGAAGGGACTGGCCGGTACGTGGGTGTccc > 1:18845/1‑149 (MQ=255) aaTAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCg > 4:143262/1‑149 (MQ=255) gAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGt < 6:15799/133‑1 (MQ=255) gAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGt > 5:15799/1‑133 (MQ=255) aTGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGa < 1:45613/149‑1 (MQ=255) gCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATg < 2:15502/147‑1 (MQ=255) gCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATg > 1:15502/1‑147 (MQ=255) aCCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGt < 4:110385/149‑1 (MQ=255) ac‑ga‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGGTGGAGCTTCTGTAccc > 6:224705/1‑149 (MQ=255) acacTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCccgtc > 3:58944/1‑149 (MQ=255) acttcctcCATCCACTTGATGGA‑GAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTCCCccgtccg > 7:54639/6‑149 (MQ=255) acttcctcCATCCACTTGATGGA‑GAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACAccgtccg > 7:58995/6‑149 (MQ=255) cATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTgg < 3:143262/148‑1 (MQ=255) cATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGt > 2:264451/1‑149 (MQ=255) aaGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGGTGGAGCTTCTGTACCCCGTCCGTTCTGGTCAc < 5:224705/148‑1 (MQ=255) | TGAGCGATAAGCGCTACCGCAGTGACCCGGCATACCGTGCCCGAGTCCAGGCCAAGCTCCTCCGTTCCAACGTCTTCTGACGCCTGGCCGCAATAAGAAAAACAACAATGAGCGGCTTCACCGAC‑GA‑‑ACACTT‑CTCCATCAAGTGGATGGAGGAAGTGGATGGCGTGGACTACTGGGCATCTTCGCCTTTCCGCTACTGGATCGGTCCCGAAGGGACTGGCCGGTACGTGGATGTCCCCGCTGGCTTCATCACTGATGGAGCTTCTGTACCCCGTCCGTTCTGGTCAC > NC_002947/2602937‑2603224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |