| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,631,141 | C→G | 11.3% | L202L (CTC→CTG) | lon‑II → | DNA‑binding ATP‑dependent protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,631,141 | 0 | C | G | 11.3% | 174.4 / 13.2 | 62 | L202L (CTC→CTG) | lon‑II | DNA‑binding ATP‑dependent protease |
| Reads supporting (aligned to +/- strand): ref base C (24/31); new base G (3/4); total (27/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
ACGAGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAG > NC_002947/2631002‑2631280 | acgaGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTc < 1:160909/149‑1 (MQ=255) cgaGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTc > 4:101990/1‑148 (MQ=255) gaGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCg > 7:213032/1‑148 (MQ=255) aGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCTGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGAGCTCCTGCAGGTCGa > 3:29560/1‑148 (MQ=255) aGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGaa < 5:167399/149‑1 (MQ=255) ccAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGc < 4:109474/139‑1 (MQ=255) ccAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGc > 3:109474/1‑139 (MQ=255) aGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTGCAGGAGGTCGatttcg < 3:45563/149‑6 (MQ=255) gcCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCAtt > 8:13158/1‑149 (MQ=255) acacCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg < 2:69025/149‑1 (MQ=255) acacCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg < 2:73537/149‑1 (MQ=255) acCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGt < 7:7258/149‑1 (MQ=255) ccATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGt > 7:292950/1‑148 (MQ=255) ccATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATACCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTc < 8:222545/149‑1 (MQ=255) aTGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTc > 7:293591/1‑147 (MQ=255) gCCGCGCACATTGCCCTGAAGATCGAGCAGAAGCATGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATTCCGAAATCTACCTCCTGCAGGTCTAAAAGCGCATTCGCGGCCGGGTCaaga < 2:160875/148‑1 (MQ=255) gCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGTGTCaaga < 4:213796/148‑1 (MQ=255) aTGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGa < 7:82412/149‑1 (MQ=255) gAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATg > 6:326715/1‑139 (MQ=255) gAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATg < 5:326715/139‑1 (MQ=255) gAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGa < 3:101990/149‑1 (MQ=255) gAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGa < 5:128738/149‑1 (MQ=255) aGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGc < 6:209852/126‑1 (MQ=255) aGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGc > 5:209852/1‑126 (MQ=255) gAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGa > 3:157280/1‑129 (MQ=255) gAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGa < 4:157280/129‑1 (MQ=255) aGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGtactac < 8:93576/149‑1 (MQ=255) gcaaTTCTTGAATTCCTCGAGTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGATGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCCCATTCGCGGCCGGGTCAAGAACCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGaa < 4:29560/147‑1 (MQ=255) gAAATTCTTGAAATTGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGaa > 4:143194/1‑149 (MQ=255) cTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGc < 7:286026/148‑1 (MQ=255) ttGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAg > 4:255343/1‑146 (MQ=255) tcgACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGcc < 8:293591/149‑1 (MQ=255) aCTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCaagaa > 4:188047/1‑94 (MQ=255) aCTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCaagaa < 3:188047/94‑1 (MQ=255) gACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGc < 5:133345/131‑1 (MQ=255) gACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGc > 6:133345/1‑131 (MQ=255) gACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCa < 6:54789/147‑1 (MQ=255) aCTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCaga > 1:280908/1‑148 (MQ=255) ccGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATTGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATg < 8:130984/91‑1 (MQ=255) ccGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATTGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATg > 7:130984/1‑91 (MQ=255) ccGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAAc > 4:86736/1‑149 (MQ=255) tggcGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTg < 3:169514/146‑1 (MQ=255) gAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGa > 1:264732/1‑149 (MQ=255) gCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATg < 2:264732/149‑1 (MQ=255) gTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGa < 8:213032/149‑1 (MQ=255) gcgcTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCagaaag > 4:289121/1‑127 (MQ=255) gcgcTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCagaaag < 3:289121/127‑1 (MQ=255) cctgcagGAGGTCGATTTCGGCATCCAGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGc > 5:21953/2‑148 (MQ=255) ctgGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCcaca < 2:280908/148‑1 (MQ=255) ctgGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGACCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACaa > 4:140231/1‑149 (MQ=255) tgGATGCCGAAATCGTCCTCCTTCAGGTCGAAAAGCGCATTCGCGGCCGTGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTTAATGAGCAGATGAAGGCCATTCAGAAAGAACtt < 8:295474/125‑1 (MQ=255) tgGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACtt > 7:295474/1‑125 (MQ=255) gATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGAATTCGCGGCCGGGTCATGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACATCAATGAGCAGATGAAGGCAATTCAGATAGAACTTGGCGATGGTGACGAAGGCCACAATg > 6:319969/1‑148 (MQ=255) gcttttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTc > 2:89926/6‑148 (MQ=255) gcttttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTc < 1:89926/143‑1 (MQ=255) cGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAAt < 6:313875/142‑1 (MQ=255) cGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAAt > 5:313875/1‑142 (MQ=255) ttttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCg > 8:152044/4‑147 (MQ=255) ttttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCg > 8:156550/4‑147 (MQ=255) aaTCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAag < 8:6439/149‑1 (MQ=255) ttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAag < 7:152044/147‑1 (MQ=255) ttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAag < 7:156550/147‑1 (MQ=255) | ACGAGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAG > NC_002947/2631002‑2631280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |