| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,703,531 | T→C | 11.9% | L230L (TTG→CTG) | PP_2369 → | leucine‑rich repeat‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,703,531 | 0 | T | C | 11.9% | 79.2 / 4.0 | 34 | L230L (TTG→CTG) | PP_2369 | leucine‑rich repeat‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (13/16); new base C (2/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGACCCGTATGCCCCGGTGAGTTGGCACACCAGCTGG > NC_002947/2703388‑2703674 | gCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAg < 1:153446/149‑1 (MQ=255) cACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAgt < 6:167761/149‑1 (MQ=255) ccTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAgtgt > 1:2782/1‑148 (MQ=255) ccTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAgtgt > 8:106584/1‑148 (MQ=255) ccTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAgtgt > 3:75625/1‑148 (MQ=255) ggCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGc < 3:310082/149‑1 (MQ=255) ggCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCTACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGtt > 4:144849/1‑123 (MQ=255) ggCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGtt < 3:144849/123‑1 (MQ=255) cTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAag > 4:26720/1‑147 (MQ=255) atccTTGCGGCGATTGCAGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTGTTTCCTGCCAGCCGACAACGCCGTGGTGAAGTGTCCCACCCTGCGGGTGTTGGGGAAGGAGGt > 6:82635/4‑149 (MQ=255) atccTTGCGGCGATTGCAGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAagga < 7:160237/143‑1 (MQ=255) atccTTGCGGCGATTGCAGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAagga > 8:160237/4‑146 (MQ=255) gctgcAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTg < 2:2782/148‑1 (MQ=255) aaggatcaaggatGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACGGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTgggg > 4:248216/1‑121 (MQ=255) aaggatcaaggatGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACCGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTgggg < 3:248216/121‑1 (MQ=255) gatcaaggatGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAg < 7:106584/149‑1 (MQ=255) aaggatGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGc < 2:36155/148‑1 (MQ=255) gATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGa > 3:104119/1‑127 (MQ=255) gATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGa < 4:104119/127‑1 (MQ=255) aGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCtt < 3:26720/149‑1 (MQ=255) aGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGt < 7:278527/122‑1 (MQ=255) aGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGt > 8:278527/1‑122 (MQ=255) gATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCGAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGt > 4:147552/1‑149 (MQ=255) aaCCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGCTGAAGTGTCACTCCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAg < 2:126400/109‑1 (MQ=255) aaCCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGCTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAg > 1:130990/1‑109 (MQ=255) aaCCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGCTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAg < 2:130990/109‑1 (MQ=255) aaCCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGCTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAg > 1:126400/1‑109 (MQ=255) aGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCt > 7:47507/1‑149 (MQ=255) gCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGa < 8:47507/149‑1 (MQ=255) cGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAg < 4:75625/149‑1 (MQ=255) tGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGAc < 3:147552/149‑1 (MQ=255) tCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCCGGCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGACCCGTATGccc < 1:224368/149‑1 (MQ=255) aCAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGACCCGTATGCCCCGGTGAGTTGGCacac > 2:7260/1‑148 (MQ=255) cGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGACCCGTATGCCCCGGTGAGTTGGCACACCAGCt > 3:261941/1‑149 (MQ=255) gCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGACCCGTATGCCCCGGTGAGTTGGCACACCAGCTg > 8:38340/1‑149 (MQ=255) ccGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGACCCGTATGCCCCGGTGAGTTGGCACACCAGCTgg < 7:38340/149‑1 (MQ=255) | GCACCCTGGAGGAGGCGTTGAGGGCCAACCTGGCGCTTGCTGCAATCGCCGCAAGGATCAAGGATGAAATCGATGAGCAGACCTGTCAGTGGATCAACCAGGCGGTGGGCACAGCGTCTTTCCTGCCAGCCGACAACGCCGTGTTGAAGTGTCACACCCTGCGGCTGTTGGGGAAGGAGGTCATTGGCGCACTGGTGATCGAAGTGCGTCAGAACGCCC‑GCCTGCTTGGCGTTATCGCGTGGTTCCCTGAGGACCCGTATGCCCCGGTGAGTTGGCACACCAGCTGG > NC_002947/2703388‑2703674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |