Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,753,883 A→C 12.7% E70A (GAG→GCG)  czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,753,8830AC12.7% 145.3 / 12.0 54E70A (GAG→GCG) czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (21/26);  new base C (3/4);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCTGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAG  >  NC_002947/2753735‑2754031
                                                                                                                                                    |                                                                                                                                                    
gTCCTGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCga                                                                                                                                                      <  3:231290/149‑1 (MQ=255)
   cTGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCgaga                                                                                                                                                    <  5:34923/148‑1 (MQ=255)
         gCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAt                                                                                                                                             >  5:35521/1‑149 (MQ=255)
           cgcgGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAtgg                                                                                                                                           <  8:60986/149‑1 (MQ=255)
           cgcgGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAtgg                                                                                                                                           <  4:63888/149‑1 (MQ=255)
            gcgGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGGCTCGAtggc                                                                                                                                          <  6:210652/149‑1 (MQ=255)
               gTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAtggcaaa                                                                                                                                       <  1:92613/149‑4 (MQ=255)
               gTGGGTAGCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                       <  7:293212/149‑4 (MQ=38)
                  ggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCt                                                                                                                                    <  7:191871/149‑1 (MQ=255)
                        cacaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAg                                                                                                                               <  4:127415/148‑1 (MQ=255)
                         acaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                             <  3:152172/149‑1 (MQ=255)
                                ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCTCCGCCCCCGGGTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTTCCATCGAGACCGCGATGGCTGGCCTGCCAGGGATCaa                                                                                                                        <  8:168111/147‑1 (MQ=255)
                                ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                       >  5:89424/1‑129 (MQ=38)
                                ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                       <  6:89424/132‑4 (MQ=38)
                                             atcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCGGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgctcgct                                                                                                         >  5:236818/1‑149 (MQ=255)
                                                     tgtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCt                                                                                                                                    >  1:207677/1‑114 (MQ=255)
                                                     tgtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCt                                                                                                                                    <  2:207677/114‑1 (MQ=255)
                                                     tgtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTc                                                                                                                          >  2:267625/1‑124 (MQ=255)
                                                     tgtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTc                                                                                                                          <  1:267625/124‑1 (MQ=255)
                                                     tgtgCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCGGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGGCCGCGATGGCTGTCCTGCCAGGGATCAAGCAGAACCGCTCGCTGTAGCGCt                                                                                                 >  3:191382/1‑149 (MQ=255)
                                                         ccGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCggg                                                                                             >  5:25551/1‑149 (MQ=255)
                                                          cGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGc                                                                                            <  6:39507/149‑1 (MQ=255)
                                                                   cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                   <  5:194115/149‑1 (MQ=255)
                                                                           cAGGTGCAGATCAACACCGCCGCCACCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGt                                                                           >  1:216804/1‑149 (MQ=255)
                                                                            aGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACgg                                                                            <  6:213777/147‑1 (MQ=255)
                                                                            aGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACgg                                                                            >  5:213777/1‑147 (MQ=255)
                                                                                  aGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTc                                                                                       >  1:162971/1‑130 (MQ=255)
                                                                                  aGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTc                                                                                       <  2:162971/130‑1 (MQ=255)
                                                                                       aaCACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatg                                                                <  6:35521/148‑1 (MQ=255)
                                                                                        acacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGTGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatgat                                                              >  4:87503/1‑149 (MQ=255)
                                                                                        acacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                   <  3:121571/128‑1 (MQ=255)
                                                                                        acacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                   >  4:121571/1‑128 (MQ=255)
                                                                                        acacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatgat                                                              <  6:154222/149‑1 (MQ=255)
                                                                                        acacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatgat                                                              <  6:25551/149‑1 (MQ=255)
                                                                                         cacCGCCGCCCCGGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGatgatg                                                             <  6:236818/149‑1 (MQ=255)
                                                                                            cgccgcCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGc                                                                                            <  1:218889/115‑1 (MQ=255)
                                                                                            cgccgcCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGc                                                                                            >  2:218889/1‑115 (MQ=255)
                                                                                                 cccccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGa                                                      >  5:307730/1‑148 (MQ=255)
                                                                                                      ggCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtctt                                                <  4:276619/149‑1 (MQ=255)
                                                                                                         tATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATcttctt                                             >  1:282033/1‑149 (MQ=255)
                                                                                                          aTTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCttcttc                                            >  6:112447/1‑149 (MQ=255)
                                                                                                           ttCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGAACCGATATCttcttc                                            <  2:216804/148‑1 (MQ=255)
                                                                                                              gccgcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgccc                                        >  1:159451/1‑149 (MQ=255)
                                                                                                                 gcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCga                                                                  >  5:219209/1‑120 (MQ=255)
                                                                                                                 gcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCga                                                                  <  6:219209/120‑1 (MQ=255)
                                                                                                                 gcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgcccgc                                      >  6:224043/1‑148 (MQ=255)
                                                                                                                                gcgcATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAgc                      <  2:159451/149‑1 (MQ=255)
                                                                                                                                    aTCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCt                  >  4:138805/1‑149 (MQ=255)
                                                                                                                                        ccTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAg              <  6:307730/149‑1 (MQ=255)
                                                                                                                                         cTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAg                                                                                  >  2:301380/1‑80 (MQ=255)
                                                                                                                                         cTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAg                                                                                  <  1:301380/80‑1 (MQ=255)
                                                                                                                                           cagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCttcttc                                            <  2:38114/114‑1 (MQ=255)
                                                                                                                                           cagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCttcttc                                            >  1:38114/3‑116 (MQ=255)
                                                                                                                                           cagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTg           >  1:152177/3‑149 (MQ=255)
                                                                                                                                            tGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTg           <  2:282033/148‑1 (MQ=255)
                                                                                                                                                aTCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcg      >  2:285362/1‑149 (MQ=255)
                                                                                                                                                    agaCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAg  >  7:237921/1‑149 (MQ=255)
                                                                                                                                                    agaCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAg  >  1:39431/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTCCTGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAG  >  NC_002947/2753735‑2754031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑