| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,812,775 | C→T | 13.9% | intergenic (+69/‑41) | rplT → / → pheS | 50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,812,775 | 0 | C | T | 13.9% | 87.4 / 6.5 | 36 | intergenic (+69/‑41) | rplT/pheS | 50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (13/18); new base T (2/3); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
CTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGG > NC_002947/2812632‑2812922 | cTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCa < 5:102115/148‑1 (MQ=255) tGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGaag > 7:276119/1‑148 (MQ=255) tCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTCGAAGGCTCTTc > 5:87014/1‑149 (MQ=255) cTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGCTCTTc < 8:276119/148‑1 (MQ=255) ggCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGc > 6:20507/1‑141 (MQ=255) ggCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGc < 5:20507/141‑1 (MQ=255) gCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGCTCTTc < 6:130098/145‑1 (MQ=255) gCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTCGAAGGCTCTTc < 6:87014/145‑1 (MQ=255) aGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTAt < 6:118252/149‑1 (MQ=255) aCGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTc < 4:34520/148‑1 (MQ=255) aCGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCg > 5:62887/1‑149 (MQ=255) gAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTa > 3:18389/1‑149 (MQ=255) aaaaaGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCTGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGTGTAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTAt < 7:47478/149‑1 (MQ=255) ggcgTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGt < 3:17534/149‑1 (MQ=255) tgcGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATg > 1:18202/1‑149 (MQ=255) tCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt < 8:168445/149‑1 (MQ=255) gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt < 4:27664/147‑1 (MQ=255) gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt > 3:27664/1‑147 (MQ=255) agaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGa < 2:18202/149‑1 (MQ=255) aaaGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTg > 7:69443/1‑145 (MQ=255) aaaGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTg < 8:69443/145‑1 (MQ=255) cGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTcc < 3:159488/149‑1 (MQ=255) cTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCt < 4:18389/149‑1 (MQ=255) tAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTgg < 4:29527/125‑1 (MQ=255) tAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTgg > 3:29527/1‑125 (MQ=255) cggcggTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTg > 5:83457/1‑146 (MQ=255) cggcggTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa < 6:83457/148‑1 (MQ=255) gTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATgcg > 1:5814/1‑104 (MQ=255) gTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATgcg < 2:5814/104‑1 (MQ=255) cgcTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa > 6:28288/1‑136 (MQ=255) cgcTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa < 5:28288/136‑1 (MQ=255) cGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGtt < 2:273424/149‑1 (MQ=255) tAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTAtctc > 7:14460/1‑149 (MQ=255) gggAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCt > 6:26613/1‑121 (MQ=255) gggAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCt < 5:26613/121‑1 (MQ=255) gAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGc > 2:17979/1‑147 (MQ=255) ccTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAggg < 6:62887/149‑1 (MQ=255) | CTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGG > NC_002947/2812632‑2812922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |