Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,812,778 C→G 12.5% intergenic (+72/‑38) rplT → / → pheS 50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,812,7780CG12.5% 110.7 / 7.9 40intergenic (+72/‑38)rplT/pheS50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (16/19);  new base G (2/3);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGC  >  NC_002947/2812632‑2812923
                                                                                                                                                  |                                                                                                                                                 
cTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCa                                                                                                                                                  <  5:102115/148‑1 (MQ=255)
 tGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGaag                                                                                                                                                >  7:276119/1‑148 (MQ=255)
        tCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTCGAAGGCTCTTc                                                                                                                                         >  5:87014/1‑149 (MQ=255)
         cTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGCTCTTc                                                                                                                                         <  8:276119/148‑1 (MQ=255)
           ggCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGc                                                                                                                                              <  5:20507/141‑1 (MQ=255)
           ggCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGc                                                                                                                                              >  6:20507/1‑141 (MQ=255)
            gCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGCTCTTc                                                                                                                                         <  6:130098/145‑1 (MQ=255)
            gCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTCGAAGGCTCTTc                                                                                                                                         <  6:87014/145‑1 (MQ=255)
              aGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTAt                                                                                                                                   <  6:118252/149‑1 (MQ=255)
                   aCGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTc                                                                                                                               <  4:34520/148‑1 (MQ=255)
                   aCGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCg                                                                                                                              >  5:62887/1‑149 (MQ=255)
                     gAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTa                                                                                                                            >  3:18389/1‑149 (MQ=255)
                      aaaaaGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCTGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGTGTAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTAt                                                                                                                           <  7:47478/149‑1 (MQ=255)
                             ggcgTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGt                                                                                                                    <  3:17534/149‑1 (MQ=255)
                                      tgcGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATg                                                                                                           >  1:18202/1‑149 (MQ=255)
                                              tCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt                                                                                                   <  8:168445/149‑1 (MQ=255)
                                                gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt                                                                                                   >  3:27664/1‑147 (MQ=255)
                                                gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt                                                                                                   <  4:27664/147‑1 (MQ=255)
                                                 agaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGa                                                                                                <  2:18202/149‑1 (MQ=255)
                                                   aaaGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTg                                                                                                  <  8:69443/145‑1 (MQ=255)
                                                   aaaGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTg                                                                                                  >  7:69443/1‑145 (MQ=255)
                                                              cGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTcc                                                                                   <  3:159488/149‑1 (MQ=255)
                                                                      cTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCt                                                                           <  4:18389/149‑1 (MQ=255)
                                                                        tAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTgg                                                                                                 <  4:29527/125‑1 (MQ=255)
                                                                        tAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTgg                                                                                                 >  3:29527/1‑125 (MQ=255)
                                                                                             cggcggTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTg                                                       >  5:83457/1‑146 (MQ=255)
                                                                                             cggcggTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                     <  6:83457/148‑1 (MQ=255)
                                                                                                  gTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATgcg                                                                                            <  2:5814/104‑1 (MQ=255)
                                                                                                  gTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATgcg                                                                                            >  1:5814/1‑104 (MQ=255)
                                                                                                         cgcTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                     >  6:28288/1‑136 (MQ=255)
                                                                                                         cgcTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                     <  5:28288/136‑1 (MQ=255)
                                                                                                                          cGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGtt                       <  2:273424/149‑1 (MQ=255)
                                                                                                                                   tAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTAtctc              >  7:14460/1‑149 (MQ=255)
                                                                                                                                      gggAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCt                                       <  5:26613/121‑1 (MQ=255)
                                                                                                                                      gggAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCt                                       >  6:26613/1‑121 (MQ=255)
                                                                                                                                        gAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGc           >  2:17979/1‑147 (MQ=255)
                                                                                                                                              ccTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAggg   <  6:62887/149‑1 (MQ=255)
                                                                                                                                                ttCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGc  >  1:34488/1‑148 (MQ=255)
                                                                                                                                                ttCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGc  >  1:128670/1‑148 (MQ=255)
                                                                                                                                                 tCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAAACTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGc  >  2:17280/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGC  >  NC_002947/2812632‑2812923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑