Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,822,895 G→C 9.3% A156A (GCG→GCC)  curA → NADPH‑dependent curcumin/dihydrocurcumin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,822,8950GC 9.3% 158.2 / 7.5 54A156A (GCG→GCC) curANADPH‑dependent curcumin/dihydrocurcumin reductase
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base C (3/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

TCCGATGGAAGCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTCTAGATCACCGTGCGCCG  >  NC_002947/2822749‑2823042
                                                                                                                                                  |                                                                                                                                                   
tCCGATGGAAGCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTggcggc                                                                                                                                                   >  5:69499/1‑149 (MQ=255)
  cGATGGAAGCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTggcggcgg                                                                                                                                                 >  6:213895/1‑149 (MQ=255)
   gATGGAAGCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACACTGGTGGTggcggcggc                                                                                                                                                >  8:263517/1‑149 (MQ=255)
        aaGCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCg                                                                                                                                           >  6:157235/1‑149 (MQ=255)
         aGCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCgg                                                                                                                                          <  2:68183/149‑1 (MQ=255)
          gCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCggg                                                                                                                                         >  8:213390/1‑149 (MQ=255)
                   ccGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGgc                                                                                                                                >  7:133232/1‑149 (MQ=255)
                    cgcgTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGgcg                                                                                                                               <  7:105790/149‑1 (MQ=255)
                                        cgcATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGc                                                                                                           <  3:184615/149‑1 (MQ=255)
                                         gcATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTAACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCCCCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGc                                                                                                           >  4:263328/1‑148 (MQ=255)
                                           aTCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGc                                                                                                           >  5:152314/1‑146 (MQ=255)
                                               ttcttATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCTCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCaa                                                                                                         <  5:244347/144‑1 (MQ=255)
                                               ttcttATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCTCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCaa                                                                                                         >  6:244347/1‑144 (MQ=255)
                                               ttcttATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATc                                                                                                     >  4:290468/1‑148 (MQ=255)
                                                 cttATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCaa                                                                                                   <  3:263328/148‑1 (MQ=255)
                                                  ttATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAgg                                                                                                 <  3:21107/149‑1 (MQ=255)
                                                    aTGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGgcgc                                                                                                                              <  8:237395/118‑1 (MQ=255)
                                                    aTGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGgcgc                                                                                                                              >  7:237395/1‑118 (MQ=255)
                                                    aTGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAggg                                                                                                <  8:166849/148‑1 (MQ=255)
                                                    aTGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAg                                                                                                  <  5:213895/146‑1 (MQ=255)
                                                          tGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACtgtg                                                                                                                       <  5:66047/119‑1 (MQ=255)
                                                          tGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACtgtg                                                                                                                       >  6:66047/1‑119 (MQ=255)
                                                             gCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCCGCCGCCAccacca                                                                                                                                        <  4:241296/99‑5 (MQ=255)
                                                             gCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCCGCCGCCAccacca                                                                                                                                        >  3:241296/1‑95 (MQ=255)
                                                                tCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCTACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCAtg                                                                                        <  1:55951/144‑1 (MQ=255)
                                                                tCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCAtg                                                                                        >  2:55951/1‑144 (MQ=255)
                                                                  tttGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCgg                                                                                 >  8:67868/1‑149 (MQ=255)
                                                                  tttGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCCGCCGCCAc                                                                                                                                             >  7:265450/1‑89 (MQ=255)
                                                                  tttGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCCGCCGCCAc                                                                                                                                             <  8:265450/89‑1 (MQ=255)
                                                                    tGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCgg                                                                                 <  7:13066/147‑1 (MQ=255)
                                                                         tGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGgcgc                                                                                                                              >  5:272537/1‑97 (MQ=255)
                                                                         tGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGgcgc                                                                                                                              <  6:272537/97‑1 (MQ=255)
                                                                                  tCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGACATGAGGGCGGTGTCGCCGGTGGcgcg                                                                 >  4:83708/1‑149 (MQ=255)
                                                                                             atGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCg                                                      >  1:162208/1‑149 (MQ=255)
                                                                                                ggCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGc                                                    <  7:213390/148‑1 (MQ=255)
                                                                                                   ttGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGc                                                >  6:1199/1‑149 (MQ=255)
                                                                                                      cTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATAGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGt                                             >  7:204597/1‑149 (MQ=255)
                                                                                                         gATATTTGCCGGCCGCAGGCGGGTGAAACACTGGTGGTGGCGGCGGACACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCAAGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGAgggc                                            >  6:102383/1‑145 (MQ=255)
                                                                                                         gATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTc                                            <  3:290468/147‑1 (MQ=255)
                                                                                                          atatTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAg                                         <  6:152314/149‑1 (MQ=255)
                                                                                                           tatTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAgg                                        >  1:2241/1‑149 (MQ=255)
                                                                                                             ttGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGt                                       <  5:1199/148‑1 (MQ=255)
                                                                                                             ttGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTg                                      <  2:162208/149‑1 (MQ=255)
                                                                                                                gccggccgCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTg                                   >  5:114706/1‑149 (MQ=255)
                                                                                                                      cgCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCaa                                                                                                   >  4:171984/1‑79 (MQ=255)
                                                                                                                         aGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGAt                          <  7:67868/149‑1 (MQ=255)
                                                                                                                         aGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGAt                          <  6:114706/149‑1 (MQ=255)
                                                                                                                             gggTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCt                      <  7:263517/149‑1 (MQ=255)
                                                                                                                               gTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGt                    <  4:60889/149‑1 (MQ=255)
                                                                                                                                tGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTTGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTc                   <  6:69499/149‑1 (MQ=255)
                                                                                                                                tGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTc                   <  5:157235/149‑1 (MQ=255)
                                                                                                                                   aaCCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGATGTGCTGGGTTTTGATGCCTGTCTa                 <  8:133232/148‑1 (MQ=255)
                                                                                                                                    aCCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAAt                                                          >  8:175208/1‑106 (MQ=255)
                                                                                                                                    aCCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAAt                                                          <  7:175208/106‑1 (MQ=255)
                                                                                                                                     cccTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTc                   >  8:184724/1‑144 (MQ=255)
                                                                                                                                          gtggtggCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTCTAGATCACCg         >  8:248162/1‑149 (MQ=255)
                                                                                                                                          ccggtggCCGCCGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTCTAGATCACCg         >  8:286581/3‑149 (MQ=255)
                                                                                                                                                  ggcggcCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTCTAGATCACCGTGCGCCg  >  2:94683/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCCGATGGAAGCGATCTGACCGCGTTGGGTGAGTCGCCGGCGCATCCTTCTTATGCCTTGGGCATCTTTGGCATGCCGGGCTTCACCGCTTATATGGGCTTGCTCGATATTGGCCGGCCGCAGGCGGGTGAAACCCTGGTGGTGGCGGCGGCCACCGGGCCGGTCGGCGCGACTGTGGGGCAGATCGGCAAGATCAAGGGCTGCCATGTGGTCGGTGTCGCCGGTGGCGCGGAAAAATGCCGGCATGCGGTCGAGGTGCTGGGTTTTGATGCCTGTCTAGATCACCGTGCGCCG  >  NC_002947/2822749‑2823042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑