Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 326,068 T→G 7.5% A555A (GCA→GCC)  PP_0269 ← glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947326,0680TG 7.5% 191.0 / 7.8 67A555A (GCA→GCC) PP_0269glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (30/32);  new base G (2/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01

GATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGGT  >  NC_002947/325920‑326215
                                                                                                                                                     |                                                                                                                                                   
gATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTAt                                                                                                                                                     >  4:253608/1‑149 (MQ=255)
gATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTAt                                                                                                                                                     >  6:35337/1‑149 (MQ=255)
     aGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCgg                                                                                                                                                <  6:78089/149‑1 (MQ=255)
       cTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCgggg                                                                                                                                              <  4:167386/149‑1 (MQ=255)
          cGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCAGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGCt                                                                                                                                            <  1:90677/149‑1 (MQ=255)
           gTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGt                                                                                                                                          >  4:135565/1‑149 (MQ=255)
            tAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGtt                                                                                                                                         >  1:110273/1‑149 (MQ=255)
               cTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGCCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTACTGATACTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCAGTTTCt                                                                                                                                      >  1:112110/1‑149 (MQ=255)
                  gTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtttt                                                                                                                                   >  1:191093/1‑149 (MQ=255)
                  gTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCttt                                                                                                                                    <  3:253608/148‑1 (MQ=255)
                  gTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGCTGttcgata                                                                                                                                    <  8:273350/148‑6 (MQ=255)
                      ttGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGc                                                                                                                               >  7:57448/1‑149 (MQ=255)
                        gCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGc                                                                                                                               <  5:35337/147‑1 (MQ=255)
                          gTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAAc                                                                                                                           >  6:126300/1‑149 (MQ=255)
                             ccAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑TATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGt                                                                                                                        <  7:223053/149‑1 (MQ=255)
                               aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCg                                                                                                                                 <  5:281820/138‑1 (MQ=255)
                               aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCg                                                                                                                                 >  6:281820/1‑138 (MQ=255)
                               aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa                                                                                                                      >  5:173009/1‑149 (MQ=255)
                                acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa                                                                                                                      <  3:137072/148‑1 (MQ=255)
                                acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa                                                                                                                      >  4:137072/1‑148 (MQ=255)
                                 caacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTc                                                                                                                                  >  2:81004/1‑135 (MQ=255)
                                 caacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTc                                                                                                                                  <  1:81004/135‑1 (MQ=255)
                                 caacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACt                                                                                                                    <  6:123982/149‑1 (MQ=255)
                                    cGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGtt                                                                                                                 <  6:173009/149‑1 (MQ=255)
                                     gCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGttt                                                                                                                <  8:57448/149‑1 (MQ=255)
                                     cccGTGCAGCTTAACGGTTAGATCGACGACGTCAACTAAGTCCGTGTATTCTTCGAGTACCC‑GTTCAGCATCTTCTTATTCTGATCTGATGTATCGAACATCCAGGGCCTATGCGGGGGGTGTTTCTTTTCGGCGATCGGTTTCTGttt                                                                                                                <  2:112110/148‑1 (MQ=255)
                                              cagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt                                                                                                                                     <  5:292143/119‑1 (MQ=255)
                                              cagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt                                                                                                                                     >  6:292143/1‑119 (MQ=255)
                                              cagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGCt                                                                                                                                            >  6:145815/1‑112 (MQ=255)
                                              cagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGCt                                                                                                                                            <  5:145815/112‑1 (MQ=255)
                                                 aaCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTg                                                                                                    >  6:227810/1‑149 (MQ=255)
                                                 aaCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTg                                                                                                    >  6:151401/1‑149 (MQ=255)
                                                      ttACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGaa                                                                                                                            >  4:215124/1‑120 (MQ=255)
                                                      ttACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGaa                                                                                                                            <  3:215124/120‑1 (MQ=255)
                                                        acacCGACGACGGCAACGCAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTa                                                                                              <  3:185596/148‑1 (MQ=255)
                                                        aCAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTAc                                                                                             >  4:17461/1‑149 (MQ=255)
                                                         cAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTAc                                                                                             <  2:191093/148‑1 (MQ=255)
                                                         cAACGACGACGGCAAAGAAGTCCGCGTTTTCGTCGAGTACCC‑GATCCGCATCTTCTGATTCTTATCTGATTTATCGAACACCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg                                                                                            <  8:165546/149‑1 (MQ=255)
                                                                gacgGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCTAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCg                                                                                     <  2:219700/149‑1 (MQ=255)
                                                                gacgGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCg                                                                                     <  5:151401/149‑1 (MQ=255)
                                                                   ggCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTg                                                                                  <  2:110273/149‑1 (MQ=255)
                                                                        cGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAg                                                                             <  5:126300/149‑1 (MQ=255)
                                                                                gcgTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCt                                                                     <  3:135565/149‑1 (MQ=255)
                                                                                        gtcgAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGcc                                                              >  8:130394/1‑148 (MQ=255)
                                                                                             gTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAACGCTGTCGCTACGCGCCATCCGc                                                        >  6:248297/1‑149 (MQ=255)
                                                                                             gTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGc                                                        >  3:282452/1‑149 (MQ=255)
                                                                                              tACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGcc                                                       <  1:210137/149‑1 (MQ=255)
                                                                                                 cc‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGtctc                                                                                       <  2:118403/114‑1 (MQ=255)
                                                                                                    gATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATc                                                  >  2:181920/1‑149 (MQ=255)
                                                                                                         gCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCcgcg                                              >  5:130424/1‑148 (MQ=255)
                                                                                                            tcttctGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTg                                                                                                    >  5:246964/1‑91 (MQ=255)
                                                                                                            tcttctGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTg                                                                                                    <  6:246964/91‑1 (MQ=255)
                                                                                                                ctgattctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATaaaa                                       <  2:211418/148‑1 (MQ=255)
                                                                                                                   attctgatCTGATGTATCGAACAGCCCCGGCCAATGCCCGGGCTGGTTGGTTTCGGCGATCGGTTACTGTTTGAGCCGGCGCAGGGCGTACGGTCTCCCGTGCAAACCTGTCGCTACGCGCCATCCCCCCCATACGCGCATAAAACTCg                                   >  8:102689/1‑149 (MQ=255)
                                                                                                                       tgatctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt                               >  3:84537/1‑149 (MQ=255)
                                                                                                                              atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatc                          >  2:127206/1‑147 (MQ=255)
                                                                                                                                 tATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGc                      >  1:66263/1‑148 (MQ=255)
                                                                                                                                     gAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACt                  <  3:17461/148‑1 (MQ=255)
                                                                                                                                      aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGa                <  6:130424/149‑1 (MQ=255)
                                                                                                                                      aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGa                <  6:226341/149‑1 (MQ=255)
                                                                                                                                      aaCAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGa                >  6:70180/1‑149 (MQ=255)
                                                                                                                                        cAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATc                                                           >  6:199976/1‑104 (MQ=255)
                                                                                                                                        cAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATc                                                           <  5:199976/104‑1 (MQ=255)
                                                                                                                                          gCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTaa            >  5:149359/1‑149 (MQ=255)
                                                                                                                                             ccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatc                          >  7:76488/1‑132 (MQ=255)
                                                                                                                                             ccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatc                          <  8:76488/132‑1 (MQ=255)
                                                                                                                                                    aTGCCGGGGCTGTTTCTTTTCGGGGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCAGATCCGCGCATAAAACTCGCTTTCGATCGAGCCAGTGAGTAACTCAc       <  8:216445/144‑1 (MQ=255)
                                                                                                                                                    aTGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGGt  >  2:234796/1‑149 (MQ=255)
                                                                                                                                                    aTGCCGGGGCTCTTTCTTTTCGGCGAACGGTTACTCTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAATCTGTCGCTACGCGCCATCCCCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGAAACTCAc       >  7:216445/1‑144 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCC‑GATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGGT  >  NC_002947/325920‑326215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑