Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,868,876 G→C 8.2% intergenic (+415/‑516) PP_2520 → / → PP_2522 hypothetical protein/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,868,8760GC 8.2% 146.7 / 2.1 48intergenic (+415/‑516)PP_2520/PP_2522hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base C (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAA  >  NC_002947/2868730‑2869024
                                                                                                                                                    |                                                                                                                                                    
aGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCggg                                                                                                                                                    <  1:14168/149‑1 (MQ=255)
     ttCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCAtt                                                                                                                                               >  5:112867/1‑149 (MQ=255)
       cAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTg                                                                                                                                              <  8:85570/148‑1 (MQ=255)
        aGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTggc                                                                                                                                            >  5:138505/1‑148 (MQ=255)
           cTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGCGCATGCGCCTTCGGGGCAGCC‑‑GATCAACTAGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTCGTGAt                                                                                                                                         >  3:82262/1‑149 (MQ=255)
               ttCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCTTCTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCt                                                                                                                                       >  1:98481/1‑149 (MQ=255)
                    gccattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGatat                                                                                                                                <  5:44182/149‑1 (MQ=255)
                        ttggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaat                                                                                                                            <  7:272399/149‑1 (MQ=255)
                           gccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaatca                                                                                                                          <  1:29170/148‑1 (MQ=255)
                                tCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaatca                                                                                                                          >  4:288114/1‑143 (MQ=255)
                                tCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaatca                                                                                                                          <  3:288114/143‑1 (MQ=255)
                                  gggTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCt                                                                                                                  <  5:62599/149‑1 (MQ=255)
                                       aaGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTcgcg                                                                                                             >  4:260915/1‑149 (MQ=255)
                                       aaGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTcgcg                                                                                                             >  4:131328/1‑149 (MQ=255)
                                            aTTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGcccc                                                                                                        >  6:86245/1‑149 (MQ=255)
                                               tGGGAGCGGCGGGGGCATGAGCGTTCGGGGCAGCC‑‑TGTCAACTCGTTGGTGCAGCTGGAGTGCGAGCTCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCACTCAGTCGCGCTTCGCGGCCCCCa                                                                                                      <  4:178383/148‑1 (MQ=255)
                                                 ggAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCAATATCAATCAGTCGCGCTTCGCGGCCCCCACg                                                                                                    <  8:168486/148‑1 (MQ=255)
                                                    gCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGt                                                                                                <  1:21142/149‑1 (MQ=255)
                                                     cGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTc                                                                                               <  6:112867/149‑1 (MQ=255)
                                                       gCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGa                                                                                             <  3:131328/149‑1 (MQ=255)
                                                          ggggCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATgc                                                                                          >  7:132029/1‑149 (MQ=255)
                                                               aTGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGAt                                                                                     >  1:193743/1‑149 (MQ=255)
                                                                tGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCAGGTTGTCGATGCGCGAtt                                                                                    <  4:82262/149‑1 (MQ=255)
                                                                  aGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGAtttt                                                                                  >  7:255298/1‑149 (MQ=255)
                                                                    ccTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGt                                                                                <  3:23299/149‑1 (MQ=255)
                                                                          gggCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCTCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACg                                                                          >  1:95432/1‑149 (MQ=255)
                                                                          gggCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACg                                                                          >  4:109969/1‑149 (MQ=255)
                                                                                     ttCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAgg                                                                 >  8:159670/1‑149 (MQ=255)
                                                                                       cAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGc                                                                <  8:255298/148‑1 (MQ=255)
                                                                                         aCTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTc                                                                    <  5:260838/142‑1 (MQ=255)
                                                                                         aCTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTc                                                                    >  6:260838/1‑142 (MQ=255)
                                                                                              ttGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTca                                                        <  2:193743/149‑1 (MQ=255)
                                                                                                     aGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACAtctc                                                  >  6:280536/1‑148 (MQ=255)
                                                                                                       cTGGAGTTCGAGCGCGGGCGGCTGCGTAACCCGTTCATGAATGCGCGGGCATTGGTGATCTGCGATATCAATCAGTGGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGAt                                               <  7:278200/149‑1 (MQ=255)
                                                                                                          gAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCaa                                            <  5:86245/149‑1 (MQ=255)
                                                                                                                       ggcggcCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCt                               <  5:280536/149‑1 (MQ=255)
                                                                                                                            ccGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTc                          <  3:109969/149‑1 (MQ=255)
                                                                                                                            ccGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTc                          >  3:140429/1‑149 (MQ=255)
                                                                                                                              gcgTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAgg                                                                 <  7:178973/108‑1 (MQ=255)
                                                                                                                              gcgTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAgg                                                                 >  8:178973/1‑108 (MQ=255)
                                                                                                                              gcgTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCg                         >  1:154883/1‑148 (MQ=255)
                                                                                                                                 tAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGa                                                <  2:139905/122‑1 (MQ=255)
                                                                                                                                 tAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGa                                                >  1:139905/1‑122 (MQ=255)
                                                                                                                                    cccGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAatat                   >  8:170206/1‑148 (MQ=255)
                                                                                                                                       gTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGt               <  2:98481/149‑1 (MQ=255)
                                                                                                                                        atcaccaATGCCCCGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGtt              >  4:300765/2‑149 (MQ=255)
                                                                                                                                          caccaATGCCCCGGCATTGATGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATAcc                                        <  1:218042/118‑1 (MQ=255)
                                                                                                                                          caccaATGCCCCGGCATTGATGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATAcc                                        >  2:218042/4‑121 (MQ=255)
                                                                                                                                             caATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTcgcg         >  6:177929/1‑149 (MQ=255)
                                                                                                                                                    ggggCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCaa  >  8:99426/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCC‑‑GTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAA  >  NC_002947/2868730‑2869024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑