Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,914,108 C→T 6.8% P1043L (CCC→CTC)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,914,1080CT 6.8% 166.6 / 2.4 59P1043L (CCC→CTC) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base T (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01

ACCGAGGACATCAGCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGTTTCGACTGG  >  NC_002947/2913963‑2914250
                                                                                                                                                  |                                                                                                                                              
accGAGGACATCAGCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCtt                                                                                                                                             >  2:193614/1‑149 (MQ=255)
     ggACATCAGCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGATTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTgg                                                                                                                                        <  2:208640/149‑1 (MQ=255)
     ggACATCAGCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTgg                                                                                                                                        <  8:191931/149‑1 (MQ=255)
        cATCAGCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTg                                                                                                                                     <  8:90560/149‑1 (MQ=255)
         aTCAGCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGa                                                                                                                                    >  3:99698/1‑149 (MQ=255)
             gCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGaa                                                                                                                                <  1:146338/149‑1 (MQ=255)
             gCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGaa                                                                                                                                <  1:193614/149‑1 (MQ=255)
              caccacCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGt                                                                                                                                      <  3:160857/142‑1 (MQ=255)
              caccacCTTCCGTGGCCTGGGCGACGACATCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGAAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACCGCGCACCTGGCGACAACTTCGCACCCTTCCTggct                                                                                                                                      >  4:160857/1‑140 (MQ=38)
                    cTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTAGCGGTAAGGAGGGTGA‑CGACTGGATCTAGAAGGGTTCCCAGGACGGCGCACCTGGCGACAACTTCGGACCCTTGCTGGGTGACGAAGTCATTg                                                                                                                         <  3:104717/149‑1 (MQ=255)
                    cTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTg                                                                                                                         >  6:232595/1‑149 (MQ=255)
                    cTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATAGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTg                                                                                                                         >  6:189970/1‑149 (MQ=255)
                        ggTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCaa                                                                                                                     <  7:257378/149‑1 (MQ=255)
                            gCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGa                                                                                                                  <  8:294198/148‑1 (MQ=255)
                               tGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATc                                                                                                              >  2:208163/1‑149 (MQ=255)
                                gggCGAAGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTAACGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCt                                                                                                             >  2:118287/1‑149 (MQ=255)
                                 ggCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCtt                                                                                                            >  2:290857/1‑149 (MQ=255)
                                  gCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTtc                                                                                                           <  1:208163/149‑1 (MQ=255)
                                    gacgacTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACTCTTGCTGGGTGACGAAGTCATTGGCAATGACATCTtcgt                                                                                                         >  6:220830/1‑149 (MQ=255)
                                    gacgacTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACTCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTtcgt                                                                                                         >  4:156574/1‑149 (MQ=255)
                                       gacTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCgg                                                                                                      <  1:261597/149‑1 (MQ=255)
                                       gacTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCgg                                                                                                      >  4:264262/1‑149 (MQ=255)
                                         cTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACTCTTGCTGGGTGACGAAGTCATTGGCAATGACATCTTCGTCgggg                                                                                                    <  5:220830/149‑1 (MQ=255)
                                         cTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACTCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCgggg                                                                                                    <  3:156574/149‑1 (MQ=255)
                                            cATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGACCGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGggc                                                                                                  >  1:233225/1‑149 (MQ=255)
                                            cATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGggcg                                                                                                 >  8:299286/1‑149 (MQ=255)
                                                 tCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGggcggcggc                                                                                            >  8:106444/1‑149 (MQ=255)
                                                      gcCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTtc                                                                                                           <  1:122823/129‑1 (MQ=255)
                                                      gcCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTtc                                                                                                           >  2:122823/1‑129 (MQ=255)
                                                      gcCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTcga                                                                                       <  1:290857/149‑1 (MQ=255)
                                                       ccAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCAtt                                                                                                                          >  3:270923/1‑113 (MQ=255)
                                                           gACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGTCGGCGGCTTCGACGaaa                                                                                  <  4:99698/149‑1 (MQ=255)
                                                           gACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGaaa                                                                                  <  2:131135/149‑1 (MQ=255)
                                                               aaCCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGAt                                                                              <  3:264262/149‑1 (MQ=255)
                                                                aCCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATc                                                                             <  1:118287/149‑1 (MQ=255)
                                                                aCCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATc                                                                             <  2:220268/149‑1 (MQ=255)
                                                                    gCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGt                                                                          >  2:182184/1‑148 (MQ=255)
                                                                     ccACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGa                                                                        <  1:62882/149‑1 (MQ=255)
                                                                              ggTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGcga                                                               <  5:71704/149‑1 (MQ=255)
                                                                              ggTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGcga                                                               >  7:86297/1‑149 (MQ=255)
                                                                               gTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGcgac                                                              >  6:164851/1‑149 (MQ=255)
                                                                                      gggTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCt                                                       <  2:233225/149‑1 (MQ=255)
                                                                                                     tCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGAt                                         >  7:61117/1‑149 (MQ=255)
                                                                                                        agaAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGcc                                      >  8:71152/1‑149 (MQ=255)
                                                                                                        agaAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGcc                                      >  4:10149/1‑149 (MQ=255)
                                                                                                            gggTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGccc                                     >  6:736/1‑146 (MQ=255)
                                                                                                              gTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGCCGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTtcgtc                                                  >  5:97622/1‑131 (MQ=255)
                                                                                                              gTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTtcgtc                                                  <  6:97622/131‑1 (MQ=255)
                                                                                                                      gACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAatgga                        >  1:173359/1‑149 (MQ=255)
                                                                                                                            gcACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCAt                  >  2:279258/1‑149 (MQ=255)
                                                                                                                               ccTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTc               <  5:232595/149‑1 (MQ=255)
                                                                                                                                   gCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCgg            <  1:279258/148‑1 (MQ=255)
                                                                                                                                    cGAAAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGtt          <  7:198623/149‑1 (MQ=255)
                                                                                                                                     gACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGttt         <  7:71152/149‑1 (MQ=255)
                                                                                                                                     gACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGttt         <  5:736/149‑1 (MQ=255)
                                                                                                                                           ttCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGTTTCGACTg   >  6:130536/1‑149 (MQ=255)
                                                                                                                                             cGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGatat                                                         <  6:9682/93‑1 (MQ=255)
                                                                                                                                             cGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGTTTCGACTgg  <  7:299286/148‑1 (MQ=255)
                                                                                                                                             cGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGTTTCGACTgg  <  5:189970/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
ACCGAGGACATCAGCACCACCTTCGGTGGCCTGGGCGACGACTTCATCCTCGGCGCCAAGACCAACCTGCCACCTACCGGTAACGAGGGTGA‑CGACTGGATCGAGAAGGGTACCCAGGACGGCGCACCTGGCGACAACTTCGCACCCTTGCTGGGTGACGAAGTCATTGGCAACGACATCTTCGTCGGGGGCGGCGGCTTCGACGAAATGATCGGTGAGGGCGGCGACGATATCTTCGTCGGCAGCGATGCCCAAGACAAAATGGATGGCATGTCCGGTTTCGACTGG  >  NC_002947/2913963‑2914250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑