Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,965,289 C→T 8.0% M446I (ATG→ATA)  PP_2595 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,965,2890CT 8.0% 143.1 / 2.4 50M446I (ATG→ATA) PP_2595ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (26/20);  new base T (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

CCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGGCGCGGGCCATTTGCCGCAC  >  NC_002947/2965144‑2965435
                                                                                                                                                  |                                                                                                                                                  
ccaccaGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCAtg                                                                                                                                                 >  3:216205/1‑149 (MQ=255)
          tACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCCGCCGTTCGAGGGTGTGTCCCAAGCAGGCCATGTGCGCCGCtt                                                                                                                                       >  1:134234/1‑149 (MQ=255)
               gTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAg                                                                                                                                   >  5:212489/1‑148 (MQ=255)
                  cTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTgc                                                                                                                                              >  8:53502/1‑134 (MQ=255)
                  cTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTgc                                                                                                                                              <  7:53502/134‑1 (MQ=255)
                     gCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCg                                                                                                                            >  3:175934/1‑149 (MQ=255)
                      ccTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCg                                                                                                                            <  4:175934/148‑1 (MQ=255)
                          aTCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGTCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGc                                                                                                                          <  1:249013/147‑1 (MQ=255)
                          aTCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTc                                                                                                                       >  8:155721/1‑149 (MQ=255)
                                    gggCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCTATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCg                                                                                                             >  6:150620/1‑149 (MQ=255)
                                    gggCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCg                                                                                                             >  1:191245/1‑149 (MQ=255)
                                      gCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCggg                                                                                                           >  8:19239/1‑149 (MQ=255)
                                         aTGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCTGGttt                                                                                                        <  4:216205/149‑1 (MQ=255)
                                            gTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGcc                                                                                                     <  6:212489/149‑1 (MQ=255)
                                            gTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGc                                                                                                      <  2:191245/148‑1 (MQ=255)
                                               acacGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTg                                                                                                       <  6:241178/144‑1 (MQ=255)
                                               acacGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTg                                                                                                       >  5:241178/1‑144 (MQ=255)
                                               acacGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTCTGTCCCAAGCCGGCTATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGa                                                                                                   <  5:150620/148‑1 (MQ=255)
                                                 acGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGTCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATAc                                                                                                >  2:19597/1‑149 (MQ=255)
                                                 acGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTg                                                                                                                   <  2:89190/130‑1 (MQ=255)
                                                 acGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTg                                                                                                                   >  1:89190/1‑130 (MQ=255)
                                                     tGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCTATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGACATCGGGTTTGCCGATACGGAt                                                                                            >  8:89805/1‑149 (MQ=255)
                                                       gCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCTATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGACATCGGGTTTGCCGATACGGAtg                                                                                           <  7:89805/148‑1 (MQ=255)
                                                       gCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGAt                                                                                            <  7:155721/147‑1 (MQ=255)
                                                              ccACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATAGGGTTTGCCGATACGGATGTTGTCGGc                                                                                   >  4:92860/1‑149 (MQ=255)
                                                               cACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGc                                                                                   >  5:271936/1‑148 (MQ=255)
                                                                  cGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGAtg                                                                               <  6:271936/149‑1 (MQ=255)
                                                                          tGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGAtg                                                                                           >  6:34963/1‑129 (MQ=255)
                                                                          tGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGAtg                                                                                           <  5:34963/129‑1 (MQ=255)
                                                                           gcgcACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTg                                                                       >  2:27571/1‑148 (MQ=255)
                                                                            cgcACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTgg                                                                      <  5:122894/148‑1 (MQ=255)
                                                                              caacaccTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCAGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGaa                                                                    >  6:305558/4‑148 (MQ=255)
                                                                                    cTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAg                                                             >  5:150036/1‑149 (MQ=255)
                                                                                            aCGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTg                                                     <  1:27571/149‑1 (MQ=255)
                                                                                                aCGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAg                                                  >  3:216216/1‑148 (MQ=255)
                                                                                                aCGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAg                                                  >  6:248481/1‑148 (MQ=255)
                                                                                                       gAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTggt                                          >  8:274001/1‑148 (MQ=255)
                                                                                                            ccccG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCgg                                                                                    >  2:290029/1‑102 (MQ=255)
                                                                                                              ccG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACtgt                                   <  5:305558/149‑1 (MQ=255)
                                                                                                                    cAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCa                                                   >  3:193142/1‑128 (MQ=255)
                                                                                                                    cAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCa                                                   <  4:193142/128‑1 (MQ=255)
                                                                                                                         gTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAgg                         <  4:216216/149‑1 (MQ=255)
                                                                                                                              aGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGgc                    >  4:179613/1‑149 (MQ=255)
                                                                                                                                  tgtgtCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGGCTCgg                <  5:121753/149‑1 (MQ=255)
                                                                                                                                  tgtgtCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGGCGCgg                <  2:134234/149‑1 (MQ=255)
                                                                                                                                  tgtgtCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGGCGCgg                <  5:121752/149‑1 (MQ=255)
                                                                                                                                        ccAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGa                                                                                                   <  6:215306/60‑1 (MQ=255)
                                                                                                                                        ccAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGa                                                                                                   >  5:215306/1‑60 (MQ=255)
                                                                                                                                          aaGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGGCGCGGGCCATTTg        >  3:155215/1‑149 (MQ=255)
                                                                                                                                                gCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGGCGCGGGCCATTTGCCGCAc  >  3:103906/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCG‑CGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGGGTTTGCCGATACGGATGTTGTCGGCGATGCTGCCCTGGAACAGCCAGGTGTCCTGCAGTACTTGGCTGACTGTGTCGGTCAGGCTGGCGCGGGCCATTTGCCGCAC  >  NC_002947/2965144‑2965435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑