| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,054,778 | T→A | 14.6% | intergenic (‑253/+55) | PP_2666 ← / ← PP_2667 | hypothetical protein/ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,054,778 | 0 | T | A | 14.6% | 75.0 / 4.9 | 33 | intergenic (‑253/+55) | PP_2666/PP_2667 | hypothetical protein/ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (10/17); new base A (2/3); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATCGCCAGCAGGGTGAACAATGCGGTCAAGCCCA > NC_002947/3054634‑3054912 | gAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATGGCGGCAGCATGCATAGATTGCTGGGGCCGCGTTGc > 8:98994/1‑149 (MQ=255) gCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCATGCGTCGCGAAAGGGCCGCGCAGCGGCCCCCGGATCTCGGCCTAATGCATAGATTTCTGGGGCCGCATTTCGGcc > 1:212749/1‑149 (MQ=37) aTGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGccc > 6:306192/1‑148 (MQ=255) tGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCAAAGCGGcccca < 7:214635/149‑3 (MQ=255) tGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCAAAGCGGcccca < 4:171138/149‑3 (MQ=255) ccTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGacac < 5:306192/149‑1 (MQ=255) gggAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAgg < 4:295117/149‑1 (MQ=255) aGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCg < 7:98994/149‑1 (MQ=255) aCTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATc < 2:208427/131‑1 (MQ=255) aCTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGAGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATc > 1:208427/1‑131 (MQ=255) tGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGcc < 4:266253/142‑1 (MQ=255) tGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCATGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCgagaacg > 3:266253/1‑143 (MQ=255) tCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCAAAGCGGcccca < 8:23676/119‑3 (MQ=255) tCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCAAAGCGGcccca > 7:23676/1‑117 (MQ=255) cTGGCGCGATCCCTGTGGGAGCGGCCATGCGTCGCGAAAAGGCCCCGCAGCGGCCCCAGGATATCGGCAACAAGCAAAGATTGCTGGGGGCGCTTTGAGGGCCAAAAGCGACACAAGGCCGCGCCCCCCCCGGTGACAAGCCacggtt > 8:150135/1‑143 (MQ=255) gcgATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAgcg < 3:170823/149‑1 (MQ=255) ccTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCttt > 6:40165/1‑85 (MQ=255) ccTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCttt < 5:40165/85‑1 (MQ=255) tgGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTgcg < 2:209731/148‑1 (MQ=255) gCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGc < 1:204296/149‑1 (MQ=255) cTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCAt > 6:269569/1‑149 (MQ=255) cgAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGt > 6:25144/1‑149 (MQ=255) gAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGtt > 6:26045/1‑149 (MQ=255) aaaGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGtt > 6:16624/1‑148 (MQ=255) ggCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGc < 8:265371/118‑1 (MQ=255) ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTg < 2:212749/149‑1 (MQ=255) cgcgCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa < 7:73899/149‑1 (MQ=255) cgcgCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa < 5:269569/149‑1 (MQ=255) aGCGGCCGCAGGATCGCGGCATCATGCATAGATTGCTGGGTCCGCTTTGCGGCCCAATCTCGACACATGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGTGGTTGAAGGTGAGGATc < 2:308871/149‑1 (MQ=255) cAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATCGCcagcag > 4:12862/1‑149 (MQ=255) tcatGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACGCGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATCGCCAGCAGGGTGAACAATGCg < 7:150135/149‑1 (MQ=255) atGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTg > 7:195118/1‑107 (MQ=255) atGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTg < 8:195118/107‑1 (MQ=255) gATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATCGCCAGCAGGGTGAACAATGCGGTCAAGccc < 5:16624/149‑1 (MQ=255) aTTGCTGGGGCCGCTTTGCGGCCAAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCATGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATCGCCAGCAGGGTGAACAATGCGGTCAAGCCCa < 7:22396/149‑1 (MQ=255) | GAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCACACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATCGCCAGCAGGGTGAACAATGCGGTCAAGCCCA > NC_002947/3054634‑3054912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |