Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,078,131 A→G 10.2% S379P (TCG→CCG)  PP_2688 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,078,1310AG10.2% 180.2 / 8.9 68S379P (TCG→CCG) PP_2688hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (31/30);  new base G (3/4);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CGAACGGGCGCAGGTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGTTCTTCGCT  >  NC_002947/3077984‑3078275
                                                                                                                                                   |                                                                                                                                                
cGAACGGGCGCAGGTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAg                                                                                                                                                 <  7:229181/149‑1 (MQ=255)
             gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGccc                                                                                                                                         <  1:36331/144‑1 (MQ=255)
             gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGccc                                                                                                                                         >  2:36331/1‑144 (MQ=255)
             gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGAcc                                                                                                                                     >  8:7750/1‑148 (MQ=255)
             gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGAcc                                                                                                                                     >  6:16667/1‑148 (MQ=255)
                gATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaag                                                                                                                                 <  1:197647/149‑3 (MQ=255)
                gATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACa                                                                                                                                 >  8:113640/1‑149 (MQ=255)
                 aTGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGa                                                                                                                                       <  4:193570/142‑1 (MQ=255)
                 aTGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGa                                                                                                                                       >  3:193570/1‑142 (MQ=255)
                  tgtgACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGcc                                                                                                                                          >  1:274365/1‑138 (MQ=255)
                  tgtgACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGcc                                                                                                                                          <  2:274365/138‑1 (MQ=255)
                               gATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGccgccg                                                                                                                   >  6:313947/1‑148 (MQ=255)
                                  gCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaag                                                                                                                                 <  8:55909/131‑3 (MQ=255)
                                  gCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaag                                                                                                                                 >  7:55909/1‑129 (MQ=255)
                                    cGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGccc                                                                                                             >  7:254882/1‑149 (MQ=255)
                                     gCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCt                                                                                                            >  4:172394/1‑149 (MQ=255)
                                        gTCGACGAAGGTCGGTCAGGGTGACAGGCAGAGGGGCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGTAAGTCCTCGATCTGGGCGTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCTGAGCCCTgg                                                                                                          <  8:260884/148‑1 (MQ=255)
                                         tCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTgg                                                                                                          <  3:172394/147‑1 (MQ=255)
                                         tCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGt                                                                                                        >  3:85302/1‑149 (MQ=255)
                                         tCGACGAAGGGCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATCGGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGt                                                                                                        <  1:101073/149‑1 (MQ=255)
                                             cgaAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTcc                                                                                                    <  6:140711/149‑1 (MQ=255)
                                                aGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                 <  1:157886/149‑1 (MQ=255)
                                                 ggTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                 <  7:113640/148‑1 (MQ=255)
                                                                cAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCggg                                                                                   >  7:138020/1‑147 (MQ=255)
                                                                 aGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAg                                                                                <  4:85302/149‑1 (MQ=255)
                                                                  ggCACCGCGGCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTGGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGGCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAgg                                                                               <  5:305055/149‑1 (MQ=255)
                                                                    cacaGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCt                                                                                                            >  6:199560/1‑118 (MQ=255)
                                                                     acaGCGTCGGTTGAGGGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTgg                                                                            <  7:7750/149‑1 (MQ=255)
                                                                            cGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACAAGTTCGGGCAGGTGGGCCAGTa                                                                     >  6:171316/1‑149 (MQ=255)
                                                                              gTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACa                                                                   <  5:16667/149‑1 (MQ=255)
                                                                              gTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCAGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                 <  8:113573/119‑1 (MQ=255)
                                                                              gTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCAGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                 >  7:113573/1‑119 (MQ=255)
                                                                                  aGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCtt                                                                                                  >  3:199403/1‑114 (MQ=255)
                                                                                  aGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCtt                                                                                                  <  4:199403/114‑1 (MQ=255)
                                                                                   gCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGa                                                              >  2:101105/1‑149 (MQ=255)
                                                                                    cGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGa                                                              >  3:119338/1‑148 (MQ=255)
                                                                                    cGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGa                                                              <  8:254882/148‑1 (MQ=255)
                                                                                      atatAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaa                                                                                                                                  >  1:270867/1‑77 (MQ=37)
                                                                                      atatAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaa                                                                                                                                  <  2:270867/78‑2 (MQ=37)
                                                                                       tataGGCGTGCGGGCGGTCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTcc                                                                                                    <  2:6203/107‑1 (MQ=255)
                                                                                       tataGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTcc                                                                                                    >  1:6203/1‑107 (MQ=255)
                                                                                         taGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGAt                                                         <  5:313947/148‑1 (MQ=255)
                                                                                              gTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATccgccg                                                   <  7:100815/149‑1 (MQ=255)
                                                                                                 cgggcgggCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCt                                                >  2:49613/1‑149 (MQ=255)
                                                                                                    gcgggcTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGc                                             <  1:55159/149‑1 (MQ=255)
                                                                                                        gcTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCACTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCa                                         >  6:123420/1‑149 (MQ=255)
                                                                                                                  gcgcgcgGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTc                               >  1:274916/1‑149 (MQ=255)
                                                                                                                    gcgcgGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCgg                             <  1:49613/149‑1 (MQ=255)
                                                                                                                               cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAg                                  >  1:262076/1‑133 (MQ=255)
                                                                                                                               cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAg                                  <  2:262076/133‑1 (MQ=255)
                                                                                                                               cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTc                               >  6:141922/1‑136 (MQ=255)
                                                                                                                               cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTc                               <  5:141922/136‑1 (MQ=255)
                                                                                                                                   aTCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTg                                              <  4:14923/117‑1 (MQ=255)
                                                                                                                                   aTCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTg                                              >  3:14923/1‑117 (MQ=255)
                                                                                                                                   aTCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGAt              >  5:118541/1‑149 (MQ=255)
                                                                                                                                       gggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGAt                                                         >  4:35097/1‑102 (MQ=255)
                                                                                                                                       gggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGAt                                                         <  3:35097/102‑1 (MQ=255)
                                                                                                                                        ggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTgg           <  4:119338/147‑1 (MQ=255)
                                                                                                                                        ggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGtt         <  8:138020/149‑1 (MQ=255)
                                                                                                                                         atgttGGTCGGGCCCGCCTCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGtt         >  1:73526/4‑148 (MQ=255)
                                                                                                                                         atgttGGTCGGGCCCGCCTCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGtt         <  2:73526/145‑1 (MQ=255)
                                                                                                                                          cTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttct       >  4:45999/1‑149 (MQ=255)
                                                                                                                                           tttGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttctt      >  1:159624/1‑149 (MQ=255)
                                                                                                                                           tttGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttctt      >  1:152023/1‑149 (MQ=255)
                                                                                                                                             tGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttcttc     <  1:101105/148‑1 (MQ=255)
                                                                                                                                               gTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAg                                                           <  2:308045/92‑1 (MQ=255)
                                                                                                                                               gTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAg                                                           >  1:308045/1‑92 (MQ=255)
                                                                                                                                                tCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGTTCTTCGCt  >  6:43253/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGAACGGGCGCAGGTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGTTCTTCGCT  >  NC_002947/3077984‑3078275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑