Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,078,139 C→T 10.9% G376E (GGG→GAG)  PP_2688 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,078,1390CT10.9% 174.7 / 9.6 64G376E (GGG→GAG) PP_2688hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/28);  new base T (3/4);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

GTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGTTCTTCGCTCAG  >  NC_002947/3077997‑3078278
                                                                                                                                              |                                                                                                                                           
gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGccc                                                                                                                                            <  1:36331/144‑1 (MQ=255)
gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGccc                                                                                                                                            >  2:36331/1‑144 (MQ=255)
gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGAcc                                                                                                                                        >  8:7750/1‑148 (MQ=255)
gTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGAcc                                                                                                                                        >  6:16667/1‑148 (MQ=255)
   gATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaag                                                                                                                                    <  1:197647/149‑3 (MQ=255)
   gATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACa                                                                                                                                    >  8:113640/1‑149 (MQ=255)
    aTGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGa                                                                                                                                          <  4:193570/142‑1 (MQ=255)
    aTGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGa                                                                                                                                          >  3:193570/1‑142 (MQ=255)
     tgtgACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGcc                                                                                                                                             >  1:274365/1‑138 (MQ=255)
     tgtgACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGcc                                                                                                                                             <  2:274365/138‑1 (MQ=255)
                  gATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGccgccg                                                                                                                      >  6:313947/1‑148 (MQ=255)
                     gCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaag                                                                                                                                    <  8:55909/131‑3 (MQ=255)
                     gCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaag                                                                                                                                    >  7:55909/1‑129 (MQ=255)
                       cGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGccc                                                                                                                >  7:254882/1‑149 (MQ=255)
                        gCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCt                                                                                                               >  4:172394/1‑149 (MQ=255)
                           gTCGACGAAGGTCGGTCAGGGTGACAGGCAGAGGGGCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGTAAGTCCTCGATCTGGGCGTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCTGAGCCCTgg                                                                                                             <  8:260884/148‑1 (MQ=255)
                            tCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTgg                                                                                                             <  3:172394/147‑1 (MQ=255)
                            tCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGt                                                                                                           >  3:85302/1‑149 (MQ=255)
                            tCGACGAAGGGCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATCGGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGt                                                                                                           <  1:101073/149‑1 (MQ=255)
                                cgaAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTcc                                                                                                       <  6:140711/149‑1 (MQ=255)
                                   aGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                    <  1:157886/149‑1 (MQ=255)
                                    ggTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                    <  7:113640/148‑1 (MQ=255)
                                                   cAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCggg                                                                                      >  7:138020/1‑147 (MQ=255)
                                                    aGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAg                                                                                   <  4:85302/149‑1 (MQ=255)
                                                     ggCACCGCGGCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTGGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGGCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAgg                                                                                  <  5:305055/149‑1 (MQ=255)
                                                       cacaGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCt                                                                                                               >  6:199560/1‑118 (MQ=255)
                                                        acaGCGTCGGTTGAGGGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTgg                                                                               <  7:7750/149‑1 (MQ=255)
                                                               cGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACAAGTTCGGGCAGGTGGGCCAGTa                                                                        >  6:171316/1‑149 (MQ=255)
                                                                 gTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACa                                                                      <  5:16667/149‑1 (MQ=255)
                                                                 gTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCAGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                    <  8:113573/119‑1 (MQ=255)
                                                                 gTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCAGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTg                                                                                                    >  7:113573/1‑119 (MQ=255)
                                                                     aGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCtt                                                                                                     >  3:199403/1‑114 (MQ=255)
                                                                     aGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCtt                                                                                                     <  4:199403/114‑1 (MQ=255)
                                                                      gCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGa                                                                 >  2:101105/1‑149 (MQ=255)
                                                                       cGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGa                                                                 >  3:119338/1‑148 (MQ=255)
                                                                       cGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGa                                                                 <  8:254882/148‑1 (MQ=255)
                                                                         atatAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaa                                                                                                                                     <  2:270867/78‑2 (MQ=37)
                                                                         atatAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGGGCCCGCCTCGACCaaa                                                                                                                                     >  1:270867/1‑77 (MQ=37)
                                                                          tataGGCGTGCGGGCGGTCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTcc                                                                                                       <  2:6203/107‑1 (MQ=255)
                                                                          tataGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTcc                                                                                                       >  1:6203/1‑107 (MQ=255)
                                                                            taGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGAt                                                            <  5:313947/148‑1 (MQ=255)
                                                                                 gTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATccgccg                                                      <  7:100815/149‑1 (MQ=255)
                                                                                    cgggcgggCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCt                                                   >  2:49613/1‑149 (MQ=255)
                                                                                       gcgggcTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGc                                                <  1:55159/149‑1 (MQ=255)
                                                                                           gcTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCACTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCa                                            >  6:123420/1‑149 (MQ=255)
                                                                                                     gcgcgcgGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTc                                  >  1:274916/1‑149 (MQ=255)
                                                                                                       gcgcgGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCgg                                <  1:49613/149‑1 (MQ=255)
                                                                                                                  cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAg                                     <  2:262076/133‑1 (MQ=255)
                                                                                                                  cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAg                                     >  1:262076/1‑133 (MQ=255)
                                                                                                                  cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTc                                  <  5:141922/136‑1 (MQ=255)
                                                                                                                  cTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTc                                  >  6:141922/1‑136 (MQ=255)
                                                                                                                      aTCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTg                                                 <  4:14923/117‑1 (MQ=255)
                                                                                                                      aTCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTg                                                 >  3:14923/1‑117 (MQ=255)
                                                                                                                      aTCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGAt                 >  5:118541/1‑149 (MQ=255)
                                                                                                                          gggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGAt                                                            >  4:35097/1‑102 (MQ=255)
                                                                                                                          gggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGAt                                                            <  3:35097/102‑1 (MQ=255)
                                                                                                                           ggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTgg              <  4:119338/147‑1 (MQ=255)
                                                                                                                           ggCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGtt            <  8:138020/149‑1 (MQ=255)
                                                                                                                            atgttGGTCGGGCCCGCCTCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGtt            >  1:73526/4‑148 (MQ=255)
                                                                                                                            atgttGGTCGGGCCCGCCTCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGtt            <  2:73526/145‑1 (MQ=255)
                                                                                                                             cTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttct          >  4:45999/1‑149 (MQ=255)
                                                                                                                              tttGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttctt         >  1:152023/1‑149 (MQ=255)
                                                                                                                              tttGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttctt         >  1:159624/1‑149 (MQ=255)
                                                                                                                                tGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGttcttc        <  1:101105/148‑1 (MQ=255)
                                                                                                                                  gTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAg                                                              >  1:308045/1‑92 (MQ=255)
                                                                                                                                  gTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAg                                                              <  2:308045/92‑1 (MQ=255)
                                                                                                                                   tCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGTTCTTCGCt     >  6:43253/1‑148 (MQ=255)
                                                                                                                                     gacccGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGCGGCTCCTTGACCACCAGTGCGGGCCGGTCGGCCAGTACAAGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGTTCTTCGCTCAg  <  6:217084/145‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
GTCGATGTGACGCGGTGCGATGCCGCTGTCGACGAAGGTCGGGCAGGTTGACAGGCACAGCGTCGGTTGAGCGATATAGGCGTGCGGGCGGGCTTTGATGCGCGCGCGGAAGTCCTCGATCTGGGCTTTGGTCGAGGCGGGCCCGACCAACATGCCATAGCCGCCGGAGCCCTGGGTTTCCTTGACCACCAGTTCGGGCAGGTGGGCCAGTACATGGGACAGATCCGCCGGCTTGCGGCACTGCCAGGTCGGCACGTTGTTCAGGATTGGTTCTTCGCTCAG  >  NC_002947/3077997‑3078278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑