Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,085,952 C→T 13.8% G205D (GGC→GAC)  PP_2694 ← aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,085,9520CT13.8% 67.6 / 2.9 29G205D (GGC→GAC) PP_2694aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (13/12);  new base T (2/2);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTACCCATTTCCAGCTGGCAGCGCACCAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGTTGCCGAAGGCCAGGGCCGGGGCGATTTTCCAGGCGG  >  NC_002947/3085810‑3086096
                                                                                                                                              |                                                                                                                                                
gCTACCCATTTCCAGCTGGCAGCGCACCAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCa                                                                                                                                            >  5:33454/1‑149 (MQ=255)
     ccATTTCCAGCTGGCAGCGCACCAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGTCCATCACCAgg                                                                                                                                       >  3:283023/1‑149 (MQ=255)
       aTTTCCAGCTGGCAGCGCACCAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGccc                                                                                                                                                <  5:18736/138‑1 (MQ=255)
       aTTTCCAGCTGGCAGCGCACCAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATATCGGCGGCCTGGATCAGGGCCTCGCCGACGTAGGCCCCCCGGccc                                                                                                                                                >  6:18736/1‑138 (MQ=255)
               cgggCAGCGCACCAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGAGTGGATCAGGGCCTCGCCGACGTCGGCACCACTGTCCATCACCAGGTTGa                                                                                                                                   <  3:214210/141‑1 (MQ=255)
               cTGGCAGCGCACCAGATTACCGGCGGTGGCCACCCCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATATCTGCGGACTGGATCAGGGCCACGCCGACGTCGGCACCACAGTCCATCACCACGTTGaaatatc                                                                                                                             >  4:214210/1‑144 (MQ=255)
                           cAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGc                                                                                                                 <  3:265776/149‑1 (MQ=255)
                                       ggTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCtga                                                                                                     <  7:99003/149‑1 (MQ=255)
                                            ccACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGAtt                                                                                                >  7:284094/1‑149 (MQ=255)
                                              aCCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTc                                                                                              <  6:33454/149‑1 (MQ=255)
                                                           ggCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCa                                                                                  <  1:247012/148‑1 (MQ=255)
                                                            gCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTggg                                                                                                                      >  8:97828/1‑111 (MQ=255)
                                                                   tGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGc                                                                         >  6:257168/1‑149 (MQ=255)
                                                                     cAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGcc                                                                        <  1:191799/148‑1 (MQ=255)
                                                                      agagAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCgg                                                                      >  6:43275/1‑149 (MQ=255)
                                                                                  aaCGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGc                                                          <  5:43275/149‑1 (MQ=255)
                                                                                         gggCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGTCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTg                                                   <  4:283023/149‑1 (MQ=255)
                                                                                           gCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGaa                                                 >  5:98234/1‑149 (MQ=255)
                                                                                              tCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAacac                                              <  6:98234/149‑1 (MQ=255)
                                                                                                  tcgcggctgACTGGATCAGGGCCTCGCCGACGACGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACg                                          <  8:284094/146‑1 (MQ=255)
                                                                                                      ggcggACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGt                                      >  8:288713/1‑149 (MQ=255)
                                                                                                           aCTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGccc                                                                                   >  2:15164/1‑99 (MQ=255)
                                                                                                           aCTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGccc                                                                                   <  1:15164/99‑1 (MQ=255)
                                                                                                               gATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGTTGCCGAAgg                             >  8:227890/1‑149 (MQ=255)
                                                                                                                       ccTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGTTGCCGAAGGCCAGGGcc                     >  6:310115/1‑149 (MQ=255)
                                                                                                                           gCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGTTGCCGAAGGCCAGGGCCgggg                 <  6:191677/149‑1 (MQ=255)
                                                                                                                              gACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGTTGCCGAAGGCCAGGGCCGGGGCGa              <  7:117683/149‑1 (MQ=255)
                                                                                                                               aCGTCGCCACCACTGCCCATCACCAGGTTGAATGTGCCGCTGGGCAGGCCCTCACGGCTGATGATTTCGGTGAGCGCACAGGCACTCGCCGGCACCAGGTTGGCCCGCTTGAACACCACGGCGTTGCCGAAGGCCAGGGCCGGTGAGAt             >  6:205369/1‑149 (MQ=255)
                                                                                                                                          acTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAAcacca                                            >  4:111665/1‑107 (MQ=255)
                                                                                                                                          acTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAAcacca                                            <  3:111665/107‑1 (MQ=255)
                                                                                                                                          acTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGTTGCCGAAGGCCAGGGCCGGGGCGATTTTCCAggcgg  >  3:54872/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GCTACCCATTTCCAGCTGGCAGCGCACCAGATTACCGGCGGTGGCCACCGCTACGCGTCGGCCCGTCTGCAGAGAACCGGTGAACGTCAGGGCATCAATCTCGGCGGACTGGATCAGGGCCTCGCCGACGTCGGCACCACTGCCCATCACCAGGTTGAAGGTGCCGCTGGGCAGGCCCTGACGGCTGATGATTTCGGTGAGCGCCCAGGCACTGGCCGGCACCAGGTTGGCCGGCTTGAACACCACGGCGTTGCCGAAGGCCAGGGCCGGGGCGATTTTCCAGGCGG  >  NC_002947/3085810‑3086096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑