Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,092,365 A→G 7.7% E109G (GAA→GGA)  PP_2701 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,092,3650AG 7.7% 146.9 / 2.9 52E109G (GAA→GGA) PP_2701LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base G (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

AGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCGCAGCAACCGCTGAGTCGGCTGAGCCTG  >  NC_002947/3092219‑3092510
                                                                                                                                                   |                                                                                                                                                 
aGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑Ga                                                                                                                                                   >  2:24360/1‑147 (MQ=255)
 ggCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAg                                                                                                                                                >  6:272404/1‑149 (MQ=255)
    aaGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTa                                                                                                                                              >  8:251030/1‑148 (MQ=255)
      gCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATcc                                                                                                                                           >  7:41825/1‑149 (MQ=255)
          gtCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGc                                                                                                                                       >  6:100302/1‑149 (MQ=255)
           tCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGcc                                                                                                                                      <  6:153352/149‑1 (MQ=255)
             aTCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCtt                                                                                                                                    >  5:326705/1‑149 (MQ=255)
              tCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCttt                                                                                                                                   >  3:26071/1‑149 (MQ=255)
              tCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCttt                                                                                                                                   <  6:326705/149‑1 (MQ=255)
              tCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCttt                                                                                                                                   >  1:81218/1‑149 (MQ=255)
                       ccAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGATCGAAAGTATCCTGGCCTTTCGGCGCaa                                                                                                                           <  1:164296/149‑1 (MQ=255)
                       ccAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCaa                                                                                                                           <  8:41825/148‑1 (MQ=255)
                        cAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑Gaaa                                                                                                                                                 >  4:177540/1‑125 (MQ=255)
                        cAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑Gaaa                                                                                                                                                 <  3:177540/125‑1 (MQ=255)
                               gTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCcgcg                                                                                                                  >  3:147539/1‑149 (MQ=255)
                                   tAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTg                                                                                                              <  5:100302/149‑1 (MQ=255)
                                     gagagCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTg                                                                                                              >  8:2855/1‑147 (MQ=255)
                                     gagagCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGaa                                                                                                            <  7:251030/149‑1 (MQ=255)
                                      agagCATACTCGGCCTGCAGATGGAAGCGGCGTGCATTCAGGATGCGGCGCTGGGCTCCGTGTCGATCGGGGTGAGGTCGGTGACGGAGATGCTCTATGGCGTCGAT‑GAAAGTATCCTGGCCTTTAGGCGCAAGTACCCGCGGGTGAAg                                                                                                           <  8:64484/149‑1 (MQ=255)
                                        agCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCaa                                                                                                                           >  5:38479/1‑131 (MQ=255)
                                        agCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCaa                                                                                                                           <  6:38479/131‑1 (MQ=255)
                                        agCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTg                                                                                                              >  1:280561/1‑144 (MQ=255)
                                          cATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGatca                                                                                                       >  8:143136/1‑149 (MQ=255)
                                            tACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGatcacc                                                                                                     <  5:225131/149‑1 (MQ=255)
                                            tACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGatcacc                                                                                                     <  7:143136/149‑1 (MQ=255)
                                             aCGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGatcacca                                                                                                    >  1:118085/1‑149 (MQ=255)
                                                    cTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GGAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGACGCTCACCCTCACCgc                                                                                             >  2:185799/1‑148 (MQ=255)
                                                    cTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GGAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGa                                                                                             >  2:190382/1‑149 (MQ=255)
                                                       cAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGAc                                                                                            <  4:26071/147‑1 (MQ=255)
                                                       cAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCt                                                                                          <  3:132492/149‑1 (MQ=255)
                                                               aGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGTCGCAAGTACCCGCTGGTGAAGATCACCATCACAGACCTGCGTCcca                                                                                  <  4:77365/149‑1 (MQ=255)
                                                                  ggcgAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGCGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCccagcc                                                                               <  5:272404/149‑1 (MQ=255)
                                                                        cATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGTGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATcc                                                                         <  2:280561/149‑1 (MQ=255)
                                                                         aTTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCt                                                                        >  7:161219/1‑149 (MQ=255)
                                                                         aTTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCt                                                                        <  2:209844/149‑1 (MQ=255)
                                                                          ttCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTg                                                                       <  7:158599/149‑1 (MQ=255)
                                                                          ttCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTg                                                                       >  1:21824/1‑149 (MQ=255)
                                                                           tCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑Gaaa                                                                                                                                                 >  3:182302/1‑74 (MQ=255)
                                                                           tCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑Gaaa                                                                                                                                                 <  4:182302/74‑1 (MQ=255)
                                                                             aGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCa                                                                     <  8:60815/148‑1 (MQ=255)
                                                                               gATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAgcg                                                                  >  5:177317/1‑149 (MQ=255)
                                                                                             cgcCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GGAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCa                                                    <  1:190382/149‑1 (MQ=255)
                                                                                             cgcCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GGAAGTATCCTGGCCTTGCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCa                                                    <  1:185799/149‑1 (MQ=255)
                                                                                                 gtgtCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCt                                                >  5:61865/1‑149 (MQ=255)
                                                                                                  tgtCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTg                                               <  2:81218/149‑1 (MQ=255)
                                                                                                  tgtCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTg                                               <  1:24360/149‑1 (MQ=255)
                                                                                                          ggggTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTg                                        <  4:148031/148‑1 (MQ=255)
                                                                                                           gggTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGc                                       >  3:310684/1‑148 (MQ=255)
                                                                                                                      ttGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCgca                           <  4:310684/149‑1 (MQ=255)
                                                                                                                      ttGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCgca                           <  5:126902/149‑1 (MQ=255)
                                                                                                                         aCGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCGTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATc                                                                          <  6:312610/99‑1 (MQ=255)
                                                                                                                             cGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCGCAGCAACCg                    >  5:111604/1‑149 (MQ=255)
                                                                                                                                  cTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCGCAGCAACCGCTGa                <  6:61865/148‑1 (MQ=255)
                                                                                                                                     gATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCATCCTGGACTTTGCCATTACTTCGCAGCAACCGCTGAGTc             <  7:2855/148‑1 (MQ=255)
                                                                                                                                     gATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCGCAGCAACCGCTGAGTCg            >  4:55676/1‑149 (MQ=255)
                                                                                                                                       tGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCGCAGCAACCGCTGAGTCGGc          <  3:189811/149‑1 (MQ=255)
                                                                                                                                               aT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCGCAGCAACCGCTGAGTCGGCTGAGCCTg  <  2:21824/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGGCAAGCGTGTCATCCGCCATGCCAACCTCGTGGTAGAGAGCATACGCCGCCTGCAGATCGAAGCGGCGAGCATTCAGGATGCGGCGCTGGGCGCCGTGTCGATCGGGGTGACGTCGTTGACGGCGATGCTCGATGGCGTCGAT‑GAAAGTATCCTGGCCTTTCGGCGCAAGTACCCGCGGGTGAAGATCACCATCACCGACCTGCGTCCCAGCCAGATCCTGCAGCGCTTGCGCGATGGCAGCCTGGACTTTGCCATTACTTCGCAGCAACCGCTGAGTCGGCTGAGCCTG  >  NC_002947/3092219‑3092510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑