Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,130,371 G→A 8.0% R63H (CGC→CAC)  PP_2747 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,130,3710GA 8.0% 141.3 / 2.5 50R63H (CGC→CAC) PP_2747hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

TGGCCGACAGCGCCCTGACGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATG  >  NC_002947/3130224‑3130505
                                                                                                                                                   |                                                                                                                                      
tGGCCGACAGCGCCCTGACGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTgcgc                                                                                                                                       >  5:158449/1‑149 (MQ=255)
    ttgcAGCGCCGTCAGGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCcagc                                                                                                                                   >  6:148709/4‑149 (MQ=255)
         gcgcCCTGACGGCGCTGCAACGTTGGGCCAGAGACCGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGt                                                                                                                              <  8:78521/149‑1 (MQ=255)
         gcgcCCTGACGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGt                                                                                                                              <  5:277144/149‑1 (MQ=255)
             ccTGACGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCa                                                                                                                          <  7:259451/149‑1 (MQ=255)
             ccTGACGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCACTGCGTCATCGCCGTCTCGGTGAGTGGAAGGCCTGGCGCTGGATCGATGTCCTGCGTGAGGTCGAGCGGGTTGCAGATGGCTTGCGCCAGCAGGGTTTCa                                                                                                                          >  8:124582/1‑149 (MQ=255)
              cTGACGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCAc                                                                                                                         >  3:129925/1‑149 (MQ=255)
                   ggCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGt                                                                                                                              >  1:35995/1‑139 (MQ=255)
                   ggCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGt                                                                                                                              <  2:35995/139‑1 (MQ=255)
                   ggCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCCGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTg                                                                                                                    >  7:213359/1‑149 (MQ=255)
                                  ggCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACGGGCAt                                                                                                     >  4:264694/1‑149 (MQ=255)
                                   gCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCAtt                                                                                                    >  6:212480/1‑149 (MQ=255)
                                   gCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCAtt                                                                                                    >  3:235590/1‑149 (MQ=255)
                                    ccAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCAtt                                                                                                    >  2:56935/1‑148 (MQ=255)
                                    ccAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTG‑CGATGGCTTGCACCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCAtt                                                                                                    >  5:193163/1‑147 (MQ=255)
                                      agagAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAg                                                                                                 <  8:106859/149‑1 (MQ=255)
                                        agaACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCg                                                                                               <  5:254037/149‑1 (MQ=255)
                                          aaCGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCCGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGt                                                                                             <  8:213359/149‑1 (MQ=255)
                                          aaCCCCCGACACAAAGCGCCCTGCGTCCTCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGt                                                                                             <  3:33917/149‑1 (MQ=255)
                                              cccGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCt                                                                                         >  3:240858/1‑149 (MQ=255)
                                              cccGACACAAATCGCCCTGCGTCATCGCCGACTCGGTGCCTGGAAGGCCTGGCGCTGGATCGATCTCCTGCGTCAGGTCGATCGGGTTGCCGATGGCTTGCGCCAGAAGCGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCt                                                                                         >  4:207861/1‑149 (MQ=255)
                                                 gACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTc                                                                                       >  1:53176/1‑148 (MQ=255)
                                                  acacAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTcga                                                                                     <  5:212480/149‑1 (MQ=255)
                                                       aaTCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTcgagccga                                                                                <  6:158449/149‑1 (MQ=255)
                                                       aaTCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTcgagccga                                                                                <  3:139650/149‑1 (MQ=255)
                                                       aaTCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTcgagcc                                                                                  >  1:246675/1‑147 (MQ=255)
                                                         tCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTcgagccgagc                                                                              <  5:148709/149‑1 (MQ=255)
                                                            cccTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCctg                                                                           <  4:235590/149‑1 (MQ=255)
                                                            cccTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCACCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCAtt                                                                                                    <  5:146217/124‑1 (MQ=255)
                                                            cccTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCACCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCAtt                                                                                                    >  6:146217/1‑124 (MQ=255)
                                                                gCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGa                                                                       >  1:258376/1‑149 (MQ=255)
                                                                  gTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATg                                                                     <  1:56935/149‑1 (MQ=255)
                                                                       cGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGc                                                                                                       <  8:96838/110‑1 (MQ=255)
                                                                       cGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGc                                                                                                       >  7:96838/1‑110 (MQ=255)
                                                                                ggTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTggcgg                                                        <  3:268210/148‑1 (MQ=255)
                                                                                    cGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCccac                                                   <  4:240858/149‑1 (MQ=255)
                                                                                    cGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTG‑CGATGGCTTGCACCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCccac                                                   <  6:193163/148‑1 (MQ=255)
                                                                                              cTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTggcg                                         >  1:41623/1‑149 (MQ=255)
                                                                                                  cgcTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGcc                                                                                          <  6:240925/96‑1 (MQ=255)
                                                                                                  cgcTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGcc                                                                                          >  5:240925/1‑96 (MQ=255)
                                                                                                          cGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTg                              <  2:258376/148‑1 (MQ=255)
                                                                                                           gATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGTTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTgg                             <  3:207861/148‑1 (MQ=255)
                                                                                                           gATGTGATGCGTGAGGTCGAGCGGGTTGCCGATTGCTTGCGCCAGCTGGGTTTGACGCCTGTCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTTGCGTTGGCGGCGCTCCAGCTTGGCGGCCAGAGCCTgg                             <  7:124582/148‑1 (MQ=255)
                                                                                                            aTGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGt                            >  6:45101/1‑148 (MQ=255)
                                                                                                               tgctgcGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGAt                         <  2:53176/148‑1 (MQ=255)
                                                                                                                 ctgcGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTgg                      >  2:250691/1‑149 (MQ=255)
                                                                                                                 ctgcGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTgg                      <  2:246675/149‑1 (MQ=255)
                                                                                                                             gAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCg          >  7:136655/1‑149 (MQ=255)
                                                                                                                               gCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTg        <  2:41623/149‑1 (MQ=255)
                                                                                                                                  ggTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCg     >  8:259878/1‑149 (MQ=255)
                                                                                                                                      gCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATg  <  4:129925/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                      
TGGCCGACAGCGCCCTGACGGCGCTGCAACGTTGGGCCAGAGAACGCCCGACACAAATCGCCCTGCGTCATCGCCGTCTCGGTGCGTGGAAGGCCTGGCGCTGGATCGATGTGCTGCGTGAGGTCGAGCGGGTTGCCGATGGCTTGCGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATG  >  NC_002947/3130224‑3130505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑