Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,153,732 G→C 7.0% intergenic (+3/‑65) PP_2769 → / → PP_2770 branched‑chain amino acid ABC transporter permease/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,153,7320GC 7.0% 176.7 / 4.1 57intergenic (+3/‑65)PP_2769/PP_2770branched‑chain amino acid ABC transporter permease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base C (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

ATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAA  >  NC_002947/3153586‑3153880
                                                                                                                                                  |                                                                                                                                                    
aTCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGa                                                                                                                                                     <  8:195115/148‑1 (MQ=255)
     tGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTc                                                                                                                                               <  5:181442/149‑1 (MQ=255)
       ccGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCg                                                                                                                                              <  7:11440/148‑1 (MQ=255)
                tAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCtt                                                                                                                                    >  1:111146/1‑149 (MQ=255)
                tAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCtt                                                                                                                                    >  1:62169/1‑149 (MQ=255)
                  gagaACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcg                                                                                                                                  <  7:251988/149‑1 (MQ=255)
                    gaACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgc                                                                                                                                 <  6:254309/148‑1 (MQ=255)
                     aaCCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgccc                                                                                                                               >  3:143210/1‑149 (MQ=255)
                      aCCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgcccg                                                                                                                              >  5:111625/1‑149 (MQ=255)
                       ccTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgcccgc                                                                                                                             <  2:199268/149‑1 (MQ=255)
                            agaagaTTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTAt                                                                                                                        <  4:70016/149‑1 (MQ=255)
                             gaagaTTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgc                                                                                                                                 <  8:117043/139‑1 (MQ=255)
                             gaagaTTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgc                                                                                                                                 >  7:117043/1‑139 (MQ=255)
                                         cGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCa                                                                                                             <  6:88842/147‑1 (MQ=255)
                                           gCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGcc                                                                                                               >  3:166457/1‑143 (MQ=255)
                                           gCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGcc                                                                                                               <  4:166457/143‑1 (MQ=255)
                                              cGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGAttt                                                                                                      <  2:62169/149‑1 (MQ=255)
                                                cTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGt                                                                                                    <  3:281242/149‑1 (MQ=255)
                                                 tGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGt                                                                                                    >  7:250633/1‑148 (MQ=255)
                                                 tGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCATTATGCACCTGCCCACTGATTTGt                                                                                                    >  8:214346/1‑148 (MQ=255)
                                                   atcatcatcTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTaaa                                                                                                 <  7:131828/149‑1 (MQ=255)
                                                   atcatcatcTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTaaa                                                                                                 <  6:111625/149‑1 (MQ=255)
                                                      atcatcTTCCTGATCAAGGAACCCGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGa                                                                                              >  8:19529/1‑149 (MQ=255)
                                                       tcatcTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGAt                                                                                             <  7:19529/149‑1 (MQ=255)
                                                            ttCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGa                                                                                              >  1:223537/1‑143 (MQ=255)
                                                            ttCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGa                                                                                              <  2:223537/143‑1 (MQ=255)
                                                            ttCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCCAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCa                                                                                        >  6:135987/1‑149 (MQ=255)
                                                            ttCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCCAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCa                                                                                        >  6:131161/1‑149 (MQ=255)
                                                             tCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCAc                                                                                       >  8:255432/1‑149 (MQ=255)
                                                                 gATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAg                                                                                                >  5:212397/1‑136 (MQ=255)
                                                                 gATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAg                                                                                                <  6:212397/136‑1 (MQ=255)
                                                                   tCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTg                                                                                                     <  8:228290/129‑1 (MQ=255)
                                                                   tCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTg                                                                                                     >  7:228290/1‑129 (MQ=255)
                                                                    cAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTc                                                                                 >  5:118293/1‑148 (MQ=255)
                                                                     aaGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCTCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGa                                                                               <  4:298192/149‑1 (MQ=255)
                                                                      aGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCAc                                                                                                            <  3:307166/119‑1 (MQ=255)
                                                                      aGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCAc                                                                                                            >  4:307166/1‑119 (MQ=255)
                                                                      aGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAc                                                                              <  6:118293/149‑1 (MQ=255)
                                                                      aGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAc                                                                              >  8:60377/1‑149 (MQ=255)
                                                                      aGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAc                                                                              >  6:57514/1‑149 (MQ=255)
                                                                       ggAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAcc                                                                             >  2:150310/1‑149 (MQ=255)
                                                                        gAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCCAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAccc                                                                            <  5:131161/149‑1 (MQ=255)
                                                                        gAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCCAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAccc                                                                            <  5:135987/149‑1 (MQ=255)
                                                                                             ctctGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTgg                                                       >  7:76022/1‑149 (MQ=255)
                                                                                                tGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGgc                                                      <  4:143210/147‑1 (MQ=255)
                                                                                                   cAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTgg                                                 >  2:171390/1‑149 (MQ=255)
                                                                                                    aaCGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGc                                                >  8:283304/1‑149 (MQ=255)
                                                                                                           tCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTaa                                           <  8:250633/147‑1 (MQ=255)
                                                                                                                 gcgcgcTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCt                                   <  7:283304/149‑1 (MQ=255)
                                                                                                                  cgcgcTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTg                                  <  7:60377/149‑1 (MQ=255)
                                                                                                                                gCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAAc                    >  2:226666/1‑149 (MQ=255)
                                                                                                                                 ccGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACg                   >  5:131648/1‑149 (MQ=255)
                                                                                                                                   gTTGCGCGACTGCGCGATCCTACGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGCTTTGTATAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTg                 <  4:71219/149‑1 (MQ=255)
                                                                                                                                          tACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCgg          >  4:74904/1‑149 (MQ=255)
                                                                                                                                            cTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCgg          <  8:76022/147‑1 (MQ=255)
                                                                                                                                               aGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAAc     <  6:131648/149‑1 (MQ=255)
                                                                                                                                                 cGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACg    >  2:233703/1‑148 (MQ=255)
                                                                                                                                                  gAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGaa  <  1:171390/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAA  >  NC_002947/3153586‑3153880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑