Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,200,601 G→A 8.0% P279P (CCG→CCA)  PP_2807 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,200,6010GA 8.0% 135.9 / 3.1 50P279P (CCG→CCA) PP_2807hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/26);  new base A (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

TTCCTGCGCCTTGAAGAGAATCACTTCCTGTCCATTCCCGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTCGCGGTCTGGGGGGATGGGTAGAGC  >  NC_002947/3200455‑3200748
                                                                                                                                                   |                                                                                                                                                   
aaccTGCGCCTTGAAGACAAACACTTAGAGTCCATACCCCTCCAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCAGCTGGGCTGGAGTGCCATTTCGACGCTGCTGGGGAGGGAGGAAATCGATGGCCAGAAGCGCGACACGCa                                                                                                                                                   >  6:182837/3‑149 (MQ=255)
           tGAAGAGAATCACTTCCTGTCCATTCCCGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTgg                                                                                                                                        >  3:81745/1‑149 (MQ=255)
            gAAGAGAATCACTTCCTGTCCATTCCCGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTgg                                                                                                                                        <  3:251901/148‑1 (MQ=255)
            gAAGAGAATCACTTCCTGTCCATTCCCGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTgg                                                                                                                                        <  3:163304/148‑1 (MQ=255)
                              tCCATTCCCGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAACCGCCTGCTGGGCTGGAGTGCCAGTACGACGGTGCTGGGGTGGGTGGAAAACAATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTATCCg                                                                                                                      >  1:224708/1‑149 (MQ=255)
                                cATTCCCGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGc                                                                                                                   >  7:138489/1‑149 (MQ=255)
                                     ccGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCggg                                                                                                               >  7:248925/1‑148 (MQ=255)
                                      cgtcgtACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTACGCCGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACGCCTGGGCATGCCGGCATTCTCCGAGCCgggg                                                                                                              >  2:229069/1‑147 (MQ=255)
                                           aaCGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGc                                                                                                        <  8:138489/149‑1 (MQ=255)
                                             cGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCtt                                                                                                      <  7:199161/149‑1 (MQ=255)
                                               tgtTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCt                                                                                                    <  7:288481/149‑1 (MQ=255)
                                                gtTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCt                                                                                                    >  8:15646/1‑148 (MQ=255)
                                                  tCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCATATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGa                                                                                                  >  6:97118/1‑148 (MQ=255)
                                                    gCCAGCTCCCGACCGAAATGCAA‑CGCCTGCTGCGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGCTGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATaa                                                                                               <  6:195779/149‑1 (MQ=255)
                                                     ccAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAAt                                                                                              >  8:136734/1‑149 (MQ=255)
                                                      cAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAAtt                                                                                             <  2:224708/149‑1 (MQ=255)
                                                         cTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTg                                                                                          <  7:15646/149‑1 (MQ=255)
                                                            ccGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGTCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGa                                                                                       <  1:234082/149‑1 (MQ=255)
                                                            ccGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTgg                                                                                         >  7:242446/1‑147 (MQ=255)
                                                                   aaaTGCAA‑CGGCTGCTGCGCTGGTGTGCCAGTTCGACGCTGCTGGGTTGGGTGGAAATCGATGTCCAGATGCTCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTGCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGAcgc                                                                                <  5:182837/149‑1 (MQ=255)
                                                                   aaaTGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTc                                                                                                     >  4:207297/1‑128 (MQ=255)
                                                                   aaaTGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTc                                                                                                     <  3:207297/128‑1 (MQ=255)
                                                                        cAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCACAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCgaat                                                                            >  5:149352/1‑148 (MQ=255)
                                                                            cgtctgctgGGCTGGAGTGTCTGTTCGTCGCTGCTTGTGTGGTTGGAAATCGATGGCCAGATTCTCGACCCGCAGTAACTCCTGGGGATGCCGGCTTTCTACTAGTCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCtaatgaat                                                                        <  4:81745/146‑1 (MQ=255)
                                                                             gCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGaatgaatg                                                                       >  4:8277/1‑149 (MQ=255)
                                                                                     ggctggAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGaa                                                                <  5:320716/148‑1 (MQ=255)
                                                                                     ggctggAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGaa                                                                <  7:229533/148‑1 (MQ=255)
                                                                                          gAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGa                                                                                       >  4:147660/1‑120 (MQ=255)
                                                                                          gAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGa                                                                                       <  3:147660/120‑1 (MQ=255)
                                                                                           aGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCACAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCg                                                         <  6:149352/149‑1 (MQ=255)
                                                                                            gTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCATATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCgg                                                        <  5:97118/149‑1 (MQ=255)
                                                                                               ccAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGa                                                                                                                     <  5:76231/85‑1 (MQ=255)
                                                                                               ccAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGa                                                                                                                     >  6:76231/1‑85 (MQ=255)
                                                                                                  gTTCGACGCTGCTGGGGTGGGTGGAATTCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATg                                                  <  7:136734/149‑1 (MQ=255)
                                                                                                  gTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCgaa                                                                             <  4:197260/122‑1 (MQ=255)
                                                                                                  gTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCgaa                                                                             >  3:197260/1‑122 (MQ=255)
                                                                                                  gTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATg                                                  >  1:261055/1‑149 (MQ=255)
                                                                                                   ttCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATgg                                                 >  8:275415/1‑149 (MQ=255)
                                                                                                   ttCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATgg                                                 <  2:261055/149‑1 (MQ=255)
                                                                                                        cGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACt                                            <  1:7104/149‑1 (MQ=255)
                                                                                                                       tggAAATCGATGGCCAGATGCGCGACCCACAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCa                                          <  3:207542/136‑1 (MQ=255)
                                                                                                                       tggAAATCGATGGCCAGATGCGCGACCCACAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCa                                          >  4:207542/1‑136 (MQ=255)
                                                                                                                        ggAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGtt                            >  1:234545/1‑149 (MQ=255)
                                                                                                                           aaTCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTcg                         <  7:208995/149‑1 (MQ=255)
                                                                                                                             tCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTcgcg                       <  8:248925/149‑1 (MQ=255)
                                                                                                                                   gCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTCGCGGTCTgg                 <  7:275415/149‑1 (MQ=255)
                                                                                                                                     cAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTCGCGGTCTgggg               >  7:102721/1‑149 (MQ=255)
                                                                                                                                         tGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGTGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTCGCGGTCTGGGGGGAt           <  3:8277/149‑1 (MQ=255)
                                                                                                                                           cgcgACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTCGCGGTCTGGGGGGATgg         <  8:242446/149‑1 (MQ=255)
                                                                                                                                            gcgACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTTACGAGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTAGAAGTTTCGCGGTATGGGCGGATggc        >  6:86237/1‑148 (MQ=255)
                                                                                                                                                   gCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTCGCGGTCTGGGGGGATGGGTAGAGc  >  8:132502/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCCTGCGCCTTGAAGAGAATCACTTCCTGTCCATTCCCGTCGAACGTGTTCGCCAGCTCCCGAGCGAAATGCAA‑CGCCTGCTGGGCTGGAGTGCCAGTTCGACGCTGCTGGGGTGGGTGGAAATCGATGGCCAGATGCGCGACCCGCAGGAACTCCTGGGCATGCCGGCATTCTCCGAGCCGGGTAAAGGCTTCTGATAATTCTGGGAGTGACGCGAATGAATGCCTTGAACAATGCGGCCGATGGTGACTCAGCACCGTTGAAGTTTCGCGGTCTGGGGGGATGGGTAGAGC  >  NC_002947/3200455‑3200748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑