Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,214,225 C→T 7.1% A47V (GCC→GTC)  mexD → multidrug RND transporter MexD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,214,2250CT 7.1% 161.9 / 2.3 56A47V (GCC→GTC) mexDmultidrug RND transporter MexD
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base T (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

TCCAGGTTCTTCATCCATCGCCCGAACTTCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAGTGATGGTGACCTTCGAGC  >  NC_002947/3214089‑3214371
                                                                                                                                        |                                                                                                                                                  
tCCAGGTTCTTCATCCATCGCCCGAACTTCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCgg                                                                                                                                        >  8:107773/1‑149 (MQ=255)
             tccatcGCCCGAACTTCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAg                                                                                                                           <  5:93249/149‑1 (MQ=255)
             tccatcGCCCGAACTTCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAg                                                                                                                           <  3:114703/149‑1 (MQ=255)
               catcGCCCGAACTTCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTTGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGATTCGGCCAAgg                                                                                                                          >  1:120796/1‑148 (MQ=255)
                           ttCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCAtggtg                                                                                                                  <  3:258040/144‑1 (MQ=255)
                           ttCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCAtggtg                                                                                                                  >  4:258040/1‑144 (MQ=255)
                           ttCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTc                                                                                                             >  5:130814/1‑149 (MQ=255)
                                cTGGGTCGTGGCGTTGTTGATTTCACTGGCCGGTTTGCTGGTGATTCTATCCTTGCCCGTGGTCTAGTTACCTAACGTGGCGCCACCGGAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAATGTCAtggtg                                                                                                                  <  2:19105/139‑1 (MQ=255)
                                cTGGGTCGTGGCGTTGTTCATTTCACTGACCGGTTTCCTGGTGATTCCATCCTTGCACGTGGCCCAGTACACCAACGTGCCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGATTAGGCCAAGTTCAtggtg                                                                                                                  >  1:19105/1‑139 (MQ=255)
                                 tGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGAc                                                                                                       >  3:270550/1‑149 (MQ=255)
                                   ggTCGTGGCGTGGTTCAGTTCACTGGCCCGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACACCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGAcc                                                                                                      <  4:223021/148‑1 (MQ=255)
                                     tCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAg                                                                                                    >  5:177682/1‑148 (MQ=255)
                                                ttCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCaa                                                                                                                            <  4:292740/113‑1 (MQ=255)
                                                ttCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCaa                                                                                                                            <  4:288138/113‑1 (MQ=255)
                                                ttCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCaa                                                                                                                            >  3:288138/1‑113 (MQ=255)
                                                ttCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGaa                                                                                          >  5:308220/1‑147 (MQ=255)
                                                ttCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACACCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCaa                                                                                                                            >  3:292740/1‑113 (MQ=255)
                                                 tCATTTCACTGGCCGGTTTGGGTGGGATTCCATCCTTGCCCGTTGCACAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGTCGCTTCGGCCAAGGTCATTGTGGAGTCTGTGACCAGCATCATCGAACa                                                                                        <  4:268591/148‑1 (MQ=255)
                                                        aCTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTc                                                                                 >  1:163951/1‑148 (MQ=255)
                                                            gCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCAtggtg                                                                                                                  <  2:209777/111‑1 (MQ=255)
                                                            gCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCAtggtg                                                                                                                  >  1:209777/1‑111 (MQ=255)
                                                            gCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACgg                                                                            >  7:111995/1‑149 (MQ=255)
                                                                   tGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCaa                                                                      <  6:177682/148‑1 (MQ=255)
                                                                        gTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCct                                                                >  3:73912/1‑149 (MQ=255)
                                                                         tGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCctg                                                               <  7:269930/149‑1 (MQ=255)
                                                                         tGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCctg                                                               <  2:163951/149‑1 (MQ=255)
                                                                            ttCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCctgctg                                                            <  5:287053/149‑1 (MQ=255)
                                                                            ttCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCctgctg                                                            >  3:12785/1‑149 (MQ=255)
                                                                            ttCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCctgctg                                                            <  4:73912/149‑1 (MQ=255)
                                                                               cATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGTCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAATGGCGCCAAGGGCCTGCTGtac                                                         >  4:103846/1‑149 (MQ=255)
                                                                               cATCCTTGCCCGTGGCCCAGTACCCCAACCTGGCGCCACCCCAGATCTCCATCACCGTCACCTACCCCGGCCCTTCGGCCAAGGACATGCTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAATCCCGCCAAGGGCCAGCTGta                                                          >  4:108378/1‑148 (MQ=255)
                                                                                 tCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGtacta                                                       >  3:180342/1‑149 (MQ=255)
                                                                                      gCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGa                                                    <  1:141036/147‑1 (MQ=255)
                                                                                       cccGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTc                                                 <  7:107773/149‑1 (MQ=255)
                                                                                         cGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTcca                                               <  8:111995/149‑1 (MQ=255)
                                                                                              cccAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACcaa                                           >  4:282449/1‑148 (MQ=255)
                                                                                                 aGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTccacc                                             <  8:182203/143‑1 (MQ=255)
                                                                                                 aGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATAATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTccacc                                             >  7:182203/1‑143 (MQ=255)
                                                                                                      cccAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCaa                                  >  3:130532/1‑149 (MQ=255)
                                                                                                      cccAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCaa                                  >  7:175113/1‑149 (MQ=255)
                                                                                                       ccAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAAc                                 <  6:308220/149‑1 (MQ=255)
                                                                                                       ccAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAAc                                 >  6:77559/1‑149 (MQ=255)
                                                                                                                 gcCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGcc                        >  7:98986/1‑148 (MQ=255)
                                                                                                                   cACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGaa                     <  5:77559/149‑1 (MQ=255)
                                                                                                                    aCCGCAGATCTCCATCACCGTCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAATGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAg                    <  3:103846/149‑1 (MQ=255)
                                                                                                                    aCCGCAGATCTCCATCACCGTCTCCTACCCCGGCGCTTCGGCCAACGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTGGCTCAATGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAg                    <  3:108378/149‑1 (MQ=255)
                                                                                                                    aCCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCaacaac                                       >  1:12626/1‑130 (MQ=255)
                                                                                                                    aCCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCaacaac                                       <  2:12626/130‑1 (MQ=255)
                                                                                                                    aCCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAg                    >  5:158411/1‑149 (MQ=255)
                                                                                                                    aCCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAg                    <  3:205627/149‑1 (MQ=255)
                                                                                                                         aGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAGTGATg               <  6:130814/149‑1 (MQ=255)
                                                                                                                              tcCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAGTGATGGTGAc          >  8:163663/1‑149 (MQ=255)
                                                                                                                               ccATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGt                            <  5:4784/130‑1 (MQ=255)
                                                                                                                               ccATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAGTGATGGTGAcc         >  6:254546/1‑149 (MQ=255)
                                                                                                                               ccATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCCAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCAAACCGcct                            >  6:4784/1‑128 (MQ=255)
                                                                                                                                      cGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAGTGATGGTGACCTTCGAGc  <  8:98986/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
TCCAGGTTCTTCATCCATCGCCCGAACTTCGCCTGGGTCGTGGCGTTGTTCATTTCACTGGCCGGTTTGCTGGTGATTCCATCCTTGCCCGTGGCCCAGTACCCCAACGTGGCGCCACCGCAGATCTCCATCACCGCCTCCTACCCCGGCGCTTCGGCCAAGGTCATGGTGGAGTCGGTGACCAGCATCATCGAACAGTCGCTCAACGGCGCCAAGGGCCTGCTGTACTACGAGTCCACCAACAACTCCAACGGCGTGGCCGAAGTGATGGTGACCTTCGAGC  >  NC_002947/3214089‑3214371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑