Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,229,093 C→T 8.5% R184C (CGC→TGC)  PP_2829 → GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,229,0930CT 8.5% 130.8 / 2.4 48R184C (CGC→TGC) PP_2829GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base T (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCTCCAACCAGTACTGGCATGCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGCCTGGGCAT  >  NC_002947/3228953‑3229229
                                                                                                                                            |                                                                                                                                        
gctgctCCAACCAGTACTGGCATGCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGTGGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTg                                                                                                                                  <  1:238167/149‑1 (MQ=255)
                  ggCATGCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCAt                                                                                                                    <  1:133420/145‑1 (MQ=255)
                  ggCATGCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCAt                                                                                                                    >  2:133420/1‑145 (MQ=255)
                   gCATGCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGcgcg                                                                                                               >  2:315852/1‑149 (MQ=255)
                    cATGCCGATGACGTCCATCTTGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTTCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGt                                                                                                              <  5:115566/149‑1 (MQ=255)
                       gCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCt                                                                                                           >  1:15958/1‑149 (MQ=255)
                       gCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCt                                                                                                           >  7:185196/1‑149 (MQ=255)
                       gCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCt                                                                                                           >  4:185885/1‑149 (MQ=255)
                         cGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTg                                                                                                          >  4:123007/1‑148 (MQ=255)
                          gATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGa                                                                                                        <  8:185196/149‑1 (MQ=255)
                            tGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAgg                                                                                                      >  6:51323/1‑149 (MQ=255)
                              aCGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGca                                                                                                    <  6:255739/149‑1 (MQ=255)
                                gTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCg                                                                                                                        >  3:175548/1‑127 (MQ=255)
                                gTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCg                                                                                                                        <  4:175548/127‑1 (MQ=255)
                                                     cTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAAccc                                                                             >  4:85200/1‑149 (MQ=255)
                                                      tCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGAATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAAcccc                                                                            >  1:236616/1‑149 (MQ=255)
                                                         cgTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGGTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCACGAg                                                                         <  5:247471/149‑1 (MQ=255)
                                                          gTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGc                                                                        <  5:73214/149‑1 (MQ=255)
                                                                ggAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCAcg                                                                  >  5:280394/1‑149 (MQ=255)
                                                                 gagtCGCTGCTAGCGGCGCTGTCCCTTGAAGGTGGTACCGCTTTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGGGCGGCGTGGCGAGTTCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGt                                                                                     <  5:295534/129‑1 (MQ=255)
                                                                 gagaCGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGt                                                                                     >  6:295534/1‑129 (MQ=255)
                                                                        tgctAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTgg                                                                                   <  4:98314/124‑1 (MQ=255)
                                                                        tgctAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTgg                                                                                   >  3:98314/1‑124 (MQ=255)
                                                                           tAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAg                                                       >  2:68975/1‑149 (MQ=255)
                                                                                gcgcTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAgctgct                                                  <  2:15958/149‑1 (MQ=255)
                                                                                  gcTGGCCCTTCACGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTgg                                                <  5:8496/149‑1 (MQ=255)
                                                                                   cTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTggg                                               <  3:185885/149‑1 (MQ=255)
                                                                                    tGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTggg                                               >  1:294967/1‑148 (MQ=255)
                                                                                      gCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGc                                                                                  >  2:238276/1‑111 (MQ=255)
                                                                                      gCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGc                                                                                  <  1:238276/111‑1 (MQ=255)
                                                                                         cTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCg                                                                           >  3:211369/1‑115 (MQ=255)
                                                                                         cTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCg                                                                           <  4:211369/115‑1 (MQ=255)
                                                                                              aGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCgg                                    <  3:123007/149‑1 (MQ=255)
                                                                                                 tggtACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTaa                                 <  2:294967/149‑1 (MQ=255)
                                                                                                     aCCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGTCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAACTGCTGCGTAGCGAGCCCGTAAGCct                             >  6:284817/1‑149 (MQ=255)
                                                                                                      ccGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCct                             <  1:9372/148‑1 (MQ=255)
                                                                                                       cGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCctgc                           <  3:85200/149‑1 (MQ=255)
                                                                                                       cGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCctgc                           >  1:186474/1‑149 (MQ=255)
                                                                                                                      gCCCTGTTGCTGGCAAGTGTTGTGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCtt            >  1:243466/1‑149 (MQ=255)
                                                                                                                      gCCCTGTTGCTGGCAAGTGTTGTGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCtt            >  1:247246/1‑149 (MQ=255)
                                                                                                                       cccTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCAc                                                                   >  1:273866/1‑93 (MQ=255)
                                                                                                                       cccTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCAc                                                                   <  2:273866/93‑1 (MQ=255)
                                                                                                                        ccTGTTGCTGGCAAGGGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGc          <  4:201248/149‑1 (MQ=255)
                                                                                                                            ttGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGTCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGCCTg       <  5:284817/148‑1 (MQ=255)
                                                                                                                              gCTGGCAAGTGTTGTGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGCCTggg     <  2:247246/148‑1 (MQ=255)
                                                                                                                              gCTGGCAAGTGTTGTGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGCCTggg     <  2:243466/148‑1 (MQ=255)
                                                                                                                                tGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGCCTGGGCAt  >  4:163129/1‑149 (MQ=255)
                                                                                                                                  gCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGCCTGGGCAt  <  1:68975/147‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
GCTGCTCCAACCAGTACTGGCATGCCGATGACGTCCATCTGGCCCCCGATGTGCTCGCGTTGCTGGAGACGCTGCTAGCGGCGCTGGCCCTTCAAGGTGGTACCGCATTGGTGCATTCGCCCTGTTGCTGGCAAGTGTTGCGCGCCCTGGCGCGTGCCGGCATGCGCGTGCTGGAGGCACCACTGGATGCCCGTGGCAACCCCGAGCTGCACGCGTTGGCCAAGCTGCTGGGTAGCGAGCCGGTAAGCCTGCTGATCATGCCGTCTTGCCTGGGCAT  >  NC_002947/3228953‑3229229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑