Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,238,145 T→G 10.0% L195R (CTG→CGG)  PP_2836 → 2‑keto‑3‑deoxyxylonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,238,1450TG10.0% 127.8 / 5.2 48L195R (CTG→CGG) PP_28362‑keto‑3‑deoxyxylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (20/23);  new base G (3/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACT  >  NC_002947/3237998‑3238288
                                                                                                                                                   |                                                                                                                                               
gCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTg                                                                                                                                                >  1:8927/1‑149 (MQ=255)
         ccGACCTGACGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGtt                                                                                                                                       >  4:86673/1‑149 (MQ=255)
          cGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTg                                                                                                                                      <  1:222561/149‑1 (MQ=255)
           gACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGACAGCGGTTgg                                                                                                                                     <  8:132044/149‑1 (MQ=255)
               tGTCGCAGATCGTGCCCGGCTAGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCAc                                                                                                                                 >  2:183441/1‑149 (MQ=255)
                   gCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGCCCGCGGTTGGCCACGgcg                                                                                                                             >  4:95860/1‑149 (MQ=255)
                             cccGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCgg                                                                                                                   >  7:250464/1‑149 (MQ=255)
                                     ggctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCAcc                                                                                                           <  8:250464/149‑1 (MQ=255)
                                          aGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCAcccc                                                                                                         >  5:182759/1‑146 (MQ=255)
                                                 aGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCt                                                                                               <  5:277698/149‑1 (MQ=255)
                                                   cgcTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGTGCCGACATCGGCATCCACCCCAAGTCCAGCTgg                                                                                             <  2:8927/149‑1 (MQ=255)
                                                   cgcTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTgg                                                                                             <  2:161326/149‑1 (MQ=255)
                                                     cTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                           >  6:196843/1‑149 (MQ=255)
                                                     cTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGGCGCAGTACCTGGAGGTGGGTCTCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                           <  4:19547/149‑1 (MQ=255)
                                                              gTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCaga                                                                                  <  3:86673/149‑1 (MQ=255)
                                                                     tAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGaa                                                                           <  1:183441/149‑1 (MQ=255)
                                                                        agCAGGGCCTGTGGTCGCAGTACATGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACAAAGGCCCAACCGCTGACCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAACTCCACCTGGAACAACCCAGAGCCCCAAgtg                                                                        >  8:128136/1‑149 (MQ=255)
                                                                          cAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACGGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAgtggt                                                                      <  6:182759/149‑1 (MQ=255)
                                                                          cAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAgtggt                                                                      >  3:94394/1‑149 (MQ=255)
                                                                            gggCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGc                                                                    <  3:224937/149‑1 (MQ=255)
                                                                               ccTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGTCCACGGCGCCGACATCGGCATCCACCCCAAGt                                                                                                     <  5:188110/113‑1 (MQ=255)
                                                                                cTGTGGTCGCAGTACCTGGAGGTGGGTAACGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGc                                                                >  1:178421/1‑149 (MQ=255)
                                                                                 tgtgGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCg                                                                              >  2:175650/1‑134 (MQ=255)
                                                                                 tgtgGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCg                                                                              <  1:175650/134‑1 (MQ=255)
                                                                                         cAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAg                                                       >  1:248314/1‑149 (MQ=255)
                                                                                           gTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGc                                                      >  8:87339/1‑148 (MQ=255)
                                                                                               cTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGGTGTCCGCGGTTGGCCACGGCGCCTACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATgg                                                 <  7:128136/149‑1 (MQ=255)
                                                                                                  gAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGCCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCCGGCCGTGTCGAGCAATGgc                                                <  3:95860/147‑1 (MQ=255)
                                                                                                  gAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGCACACCCCAGAGCCGGAAGTGGTGCTGGCCGGGTCGCGCAATGgcag                                              >  8:115330/1‑149 (MQ=255)
                                                                                                               gCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGgcgc                                  >  1:107036/1‑148 (MQ=255)
                                                                                                                    gATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCt                            >  4:68578/1‑149 (MQ=255)
                                                                                                                     aTGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                           <  6:100023/149‑1 (MQ=255)
                                                                                                                      tGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCg                          <  7:115330/149‑1 (MQ=255)
                                                                                                                      tGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCg                          <  2:107036/149‑1 (MQ=255)
                                                                                                                         cGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGGAgtggt                                                                      <  6:129097/102‑1 (MQ=255)
                                                                                                                         cGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGGAgtggt                                                                      >  5:129097/1‑102 (MQ=255)
                                                                                                                                   aCCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                           >  7:226953/1‑135 (MQ=255)
                                                                                                                                   aCCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                           <  8:226953/135‑1 (MQ=255)
                                                                                                                                      aaGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCt          >  3:238392/1‑149 (MQ=255)
                                                                                                                                       aGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                      >  8:289105/1‑72 (MQ=255)
                                                                                                                                       aGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                      <  7:289105/72‑1 (MQ=255)
                                                                                                                                          cccAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTgcgc      <  2:178421/149‑1 (MQ=255)
                                                                                                                                           ccAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAATCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTgcgc      >  6:205744/1‑148 (MQ=255)
                                                                                                                                           ccAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGcgcg     >  5:126819/1‑149 (MQ=255)
                                                                                                                                            cAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc            <  2:182165/141‑1 (MQ=255)
                                                                                                                                            cAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc            >  1:182165/1‑141 (MQ=255)
                                                                                                                                             aaCCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAc   <  4:94394/149‑1 (MQ=255)
                                                                                                                                              aCCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACt  <  3:68578/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACT  >  NC_002947/3237998‑3238288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑