Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,238,147 T→A 13.0% S196T (TCC→ACC) ‡ PP_2836 → 2‑keto‑3‑deoxyxylonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,238,1470TA13.0% 116.9 / 4.9 47S196T (TCC→ACC) ‡PP_28362‑keto‑3‑deoxyxylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (18/22);  new base A (3/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACT  >  NC_002947/3238007‑3238288
                                                                                                                                            |                                                                                                                                             
ccGACCTGACGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGtt                                                                                                                                       >  4:86673/1‑149 (MQ=255)
 cGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTg                                                                                                                                      <  1:222561/149‑1 (MQ=255)
  gACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGACAGCGGTTgg                                                                                                                                     <  8:132044/149‑1 (MQ=255)
      tGTCGCAGATCGTGCCCGGCTAGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCAc                                                                                                                                 >  2:183441/1‑149 (MQ=255)
          gCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGCCCGCGGTTGGCCACGgcg                                                                                                                             >  4:95860/1‑149 (MQ=255)
                    cccGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCgg                                                                                                                   >  7:250464/1‑149 (MQ=255)
                            ggctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCAcc                                                                                                           <  8:250464/149‑1 (MQ=255)
                                 aGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCAcccc                                                                                                         >  5:182759/1‑146 (MQ=255)
                                        aGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCt                                                                                               <  5:277698/149‑1 (MQ=255)
                                          cgcTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGTGCCGACATCGGCATCCACCCCAAGTCCAGCTgg                                                                                             <  2:8927/149‑1 (MQ=255)
                                          cgcTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTgg                                                                                             <  2:161326/149‑1 (MQ=255)
                                            cTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                           >  6:196843/1‑149 (MQ=255)
                                            cTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGGCGCAGTACCTGGAGGTGGGTCTCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                           <  4:19547/149‑1 (MQ=255)
                                                     gTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCaga                                                                                  <  3:86673/149‑1 (MQ=255)
                                                            tAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGaa                                                                           <  1:183441/149‑1 (MQ=255)
                                                               agCAGGGCCTGTGGTCGCAGTACATGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACAAAGGCCCAACCGCTGACCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAACTCCACCTGGAACAACCCAGAGCCCCAAgtg                                                                        >  8:128136/1‑149 (MQ=255)
                                                                 cAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACGGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAgtggt                                                                      <  6:182759/149‑1 (MQ=255)
                                                                 cAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAgtggt                                                                      >  3:94394/1‑149 (MQ=255)
                                                                   gggCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGc                                                                    <  3:224937/149‑1 (MQ=255)
                                                                      ccTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGTCCACGGCGCCGACATCGGCATCCACCCCAAGt                                                                                                     <  5:188110/113‑1 (MQ=255)
                                                                       cTGTGGTCGCAGTACCTGGAGGTGGGTAACGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGc                                                                >  1:178421/1‑149 (MQ=255)
                                                                        tgtgGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCg                                                                              >  2:175650/1‑134 (MQ=255)
                                                                        tgtgGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCg                                                                              <  1:175650/134‑1 (MQ=255)
                                                                                cAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAg                                                       >  1:248314/1‑149 (MQ=255)
                                                                                  gTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGc                                                      >  8:87339/1‑148 (MQ=255)
                                                                                      cTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGGTGTCCGCGGTTGGCCACGGCGCCTACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATgg                                                 <  7:128136/149‑1 (MQ=255)
                                                                                         gAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGCCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCCGGCCGTGTCGAGCAATGgc                                                <  3:95860/147‑1 (MQ=255)
                                                                                         gAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGCACACCCCAGAGCCGGAAGTGGTGCTGGCCGGGTCGCGCAATGgcag                                              >  8:115330/1‑149 (MQ=255)
                                                                                                      gCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGgcgc                                  >  1:107036/1‑148 (MQ=255)
                                                                                                           gATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCt                            >  4:68578/1‑149 (MQ=255)
                                                                                                            aTGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                           <  6:100023/149‑1 (MQ=255)
                                                                                                             tGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCg                          <  7:115330/149‑1 (MQ=255)
                                                                                                             tGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCg                          <  2:107036/149‑1 (MQ=255)
                                                                                                                cGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGGAgtggt                                                                      <  6:129097/102‑1 (MQ=255)
                                                                                                                cGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGGAgtggt                                                                      >  5:129097/1‑102 (MQ=255)
                                                                                                                          aCCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                           >  7:226953/1‑135 (MQ=255)
                                                                                                                          aCCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                           <  8:226953/135‑1 (MQ=255)
                                                                                                                             aaGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCt          >  3:238392/1‑149 (MQ=255)
                                                                                                                              aGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                      >  8:289105/1‑72 (MQ=255)
                                                                                                                              aGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                      <  7:289105/72‑1 (MQ=255)
                                                                                                                                 cccAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTgcgc      <  2:178421/149‑1 (MQ=255)
                                                                                                                                  ccAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAATCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTgcgc      >  6:205744/1‑148 (MQ=255)
                                                                                                                                  ccAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGcgcg     >  5:126819/1‑149 (MQ=255)
                                                                                                                                   cAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc            <  2:182165/141‑1 (MQ=255)
                                                                                                                                   cAACCGCGGACAGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc            >  1:182165/1‑141 (MQ=255)
                                                                                                                                    aaCCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAc   <  4:94394/149‑1 (MQ=255)
                                                                                                                                     aCCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACt  <  3:68578/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
CCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTTGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGACT  >  NC_002947/3238007‑3238288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑