Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,269,903 G→T 8.7% L64M (CTG→ATG)  PP_2868 ← Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,269,9030GT 8.7% 137.4 / 4.1 46L64M (CTG→ATG) PP_2868Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base T (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

ATCGACGATGCCATCTTCGTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCGGGTGCCGAGGAACTGCG  >  NC_002947/3269763‑3270043
                                                                                                                                              |                                                                                                                                                
aTCGACGATGCCATCTTCGTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGa                                                                                                                                          >  2:62509/1‑149 (MQ=255)
       aTGCCATCTTCGTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACgg                                                                                                                                   <  6:144344/149‑1 (MQ=255)
           cATCTTCGTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGCTGTTCAACATCGccc                                                                                                                                             <  3:256851/135‑2 (MQ=255)
           cATCTTCGTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGCTGTTCAACATCGccc                                                                                                                                             >  4:256851/1‑134 (MQ=255)
                  gTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATATCCTTTGTCTTCGGGCGCGGTGGTGGCCTCGCTGGGGAATAACCTCACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGgaga                                                                                                                          <  1:94536/148‑1 (MQ=255)
                  gTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGt                                                                                                                       >  7:36047/1‑149 (MQ=255)
                  gTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGt                                                                                                                       >  2:148751/1‑149 (MQ=255)
                    aaTGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTg                                                                                                                     >  5:109244/1‑149 (MQ=255)
                        cACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTgcgc                                                                                                                  <  8:222039/148‑1 (MQ=255)
                                ggcggcTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAgctgg                                                                                                         <  8:36047/149‑1 (MQ=255)
                                     cTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGAt                                                                                                    <  6:109244/149‑1 (MQ=255)
                                        ttGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATg                                                                                                   >  7:110294/1‑147 (MQ=255)
                                        ttGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTa                                                                                                 <  1:62509/149‑1 (MQ=255)
                                            gccgcACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCt                                                                                             >  7:129272/1‑149 (MQ=255)
                                            gccgcACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCt                                                                                             >  7:132051/1‑149 (MQ=255)
                                            gccgcACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCt                                                                                             >  8:281003/1‑149 (MQ=255)
                                               gcACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCg                                                                                          >  1:170866/1‑149 (MQ=255)
                                                         gCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCa                                                                              <  2:170866/148‑1 (MQ=255)
                                                           cTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCa                                                                                   >  4:123996/1‑141 (MQ=255)
                                                           cTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCa                                                                                   <  3:123996/141‑1 (MQ=255)
                                                               gTCTTCGGGCGCGGGGGTGGCCTCGCTGGCGAAGAAC‑‑CTCTGTATGGTCACGCCCAGGCTGCTGGCGAGGTTGAACAGCGCCGGGATCGACGGGT‑ATGAGAGGTTGCGCTCCAGCTGTCTGATGTAGCCTGCGG‑‑‑TCAGTTCACtgtgt                                                                        <  8:110294/148‑1 (MQ=255)
                                                                 cTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCg                                                                     >  2:141930/1‑149 (MQ=255)
                                                                    cGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCg                                                                     <  8:132051/146‑1 (MQ=255)
                                                                     gggCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAgctggct                                                                                                       >  8:18390/1‑114 (MQ=255)
                                                                     gggCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAgctggct                                                                                                       <  7:18390/114‑1 (MQ=255)
                                                                          cgGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATTGTAACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTcc                                                            <  4:197970/149‑1 (MQ=255)
                                                                          cgGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCgg                                                                                         >  8:284239/1‑123 (MQ=255)
                                                                          cgGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCgg                                                                                         <  7:284239/123‑1 (MQ=255)
                                                                                tggCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAg                                                      <  1:148751/149‑1 (MQ=255)
                                                                                tggCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAg                                                      <  7:281003/149‑1 (MQ=255)
                                                                                    cTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCa                                                  >  2:113024/1‑149 (MQ=255)
                                                                                    cTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAAAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCa                                                  >  3:53053/1‑149 (MQ=255)
                                                                                               ataaC‑‑CACTGGATTGGCACGCCCAGGCTGCGGGCGATTTTGAACAGCGCCGTGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGgcgc                                       <  6:42206/147‑1 (MQ=255)
                                                                                                   c‑‑cACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCa                                                                                   <  7:125575/101‑1 (MQ=255)
                                                                                                        cTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTgcg                                  >  2:192963/1‑147 (MQ=255)
                                                                                                           gATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGc                             >  1:291847/1‑149 (MQ=255)
                                                                                                                tCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGTCAGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCcgcg                         >  1:233259/1‑149 (MQ=255)
                                                                                                                 cACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCcg                           >  7:113107/1‑145 (MQ=255)
                                                                                                                 cACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCcg                           <  8:113107/145‑1 (MQ=255)
                                                                                                                     cccAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCg                   <  2:233259/149‑1 (MQ=255)
                                                                                                                              ccggCGCTGTTCAACATCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCg                   <  4:51816/138‑1 (MQ=255)
                                                                                                                              ccggCGCTGTTCAACATCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCg                   >  3:51816/3‑140 (MQ=255)
                                                                                                                              cGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCGGGTGCCGAg          <  2:291847/149‑1 (MQ=255)
                                                                                                                                ggCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCaa                                                       <  4:282541/102‑1 (MQ=255)
                                                                                                                                     tGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGGGGAACAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCGGGAGCCGAGGAAc      >  1:256513/1‑149 (MQ=255)
                                                                                                                                      gTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCGGGTGCCGAGGAACTgcg  >  6:111560/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                
ATCGACGATGCCATCTTCGTAATGCACGCTCAGGCGGCTGTTGCGCCGCACCACATAGCCTTGGTCTTCGGGCGCGGTGGTGGCCTCGCTGGCGAAGAAC‑‑CACTGGATGGTCACGCCCAGGCTGCGGGCGATGTTGAACAGCGCCGGGATCGACGGGT‑AGGAGAGGTTGCGCTCCAGCTGGCTGATGTAGCCTGCGG‑‑‑TCAGTTCACTGTGTGCGGCCAGCTCCGCCAAGGTCATGCCGCGGCGCTTGCGCAGGCCGCGGATGCGGGTGCCGAGGAACTGCG  >  NC_002947/3269763‑3270043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑