| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,330,904 | T→G | 8.6% | *366Y (TAA→TAC) | nspC ← | carboxynorspermidine/carboxyspermidine decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,330,904 | 0 | T | G | 8.6% | 168.7 / 7.8 | 58 | *366Y (TAA→TAC) | nspC | carboxynorspermidine/carboxyspermidine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base T (27/26); new base G (3/2); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
GATGTTCCGTGACGCGATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTCGAT > NC_002947/3330758‑3331047 | gaTGTTCCGTGACGCGATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTa < 1:282556/149‑1 (MQ=255) aCGCGATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTg > 2:171715/1‑148 (MQ=255) cgcgATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCTGTCGTAAGACCGGCtttc < 4:140385/149‑4 (MQ=255) cgcgATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTgg > 1:198749/1‑148 (MQ=255) gCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTa < 6:257439/148‑1 (MQ=255) gATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTc > 2:143073/1‑149 (MQ=255) tACGACCCAGTTCGTCGTTATGTTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCt > 4:166563/1‑148 (MQ=255) cGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTc > 2:265332/1‑148 (MQ=255) cccAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGaaa < 1:265332/149‑1 (MQ=255) aGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTa < 3:87495/118‑1 (MQ=255) aGTTAGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTa > 4:87495/1‑118 (MQ=255) cgtcgtTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACt > 7:163123/1‑149 (MQ=255) cgcgcACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACtt < 1:171715/148‑1 (MQ=255) cgcgcACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTc < 2:198749/149‑1 (MQ=255) gcgcACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTc > 4:167838/1‑148 (MQ=255) gtgGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGcc > 7:243532/1‑149 (MQ=255) gggCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGt > 6:149836/1‑149 (MQ=255) ggCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTc > 4:59467/1‑149 (MQ=255) gCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAAATTACCCATTGCTCCATACTTGAAAGCCGGTCTTACGACAGGCTGGAAACCTAGACTTCGAAACCGAACTCCCGGACGAATTCGACGCAGCCGTc > 6:209408/1‑148 (MQ=255) ccaccaCCTTGGCGACACCTCCTGCACCATTGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACTACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGa < 6:129849/149‑1 (MQ=255) caccacCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGAc > 6:235638/1‑137 (MQ=255) caccacCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGAc < 5:235638/137‑1 (MQ=255) caccacCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAg > 7:134248/1‑149 (MQ=255) accaccTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAgc < 8:166749/149‑1 (MQ=255) gACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCAc < 5:149836/149‑1 (MQ=255) gACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCAc > 4:67127/1‑149 (MQ=255) cacCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGa < 8:163123/149‑1 (MQ=255) acCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGAt < 8:209251/149‑1 (MQ=255) cctcctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATg < 8:243532/149‑1 (MQ=255) ctcctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATg > 2:316910/1‑148 (MQ=255) ctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGaa < 3:167838/148‑1 (MQ=255) ctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAg > 3:252649/1‑149 (MQ=255) cACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGc < 3:166563/148‑1 (MQ=255) ccAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGc < 4:121311/146‑1 (MQ=255) ccAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGc > 3:121311/1‑146 (MQ=255) cAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTGGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCt > 5:274970/1‑149 (MQ=255) cAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCt > 3:95357/1‑149 (MQ=255) aaTGATAAGAACGTTCTTCTTAAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACAGAACTCGCGGACGACTTCGACGCTGCCGTAGAGCTGCTTGAACACGATGGAAGGCATCtt > 1:181600/1‑149 (MQ=255) aCGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGtt < 5:209408/148‑1 (MQ=255) aCGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGtt < 8:134248/148‑1 (MQ=255) gTTCTTCTTCAATTTACCCTTTGCTCCTTACTAGAAAGCCCGTCTTACGACAGGCAGGAAAAGTAGTCTTCGAAACCGAACTCGCGGAAGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGaa > 5:258524/1‑149 (MQ=255) ttcAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTAGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGttt > 5:77894/1‑149 (MQ=255) cAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGtttt < 6:77894/148‑1 (MQ=255) aaTTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTc < 6:274970/149‑1 (MQ=255) tttACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCtt < 3:59467/149‑1 (MQ=255) tttACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCtt < 3:67127/149‑1 (MQ=255) cTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTaa < 1:143073/148‑1 (MQ=255) tttccaGCCTGTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACccgcc > 4:203429/6‑149 (MQ=255) ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGaa > 5:185700/1‑98 (MQ=255) ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGaa < 6:185700/98‑1 (MQ=255) ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGTATCTTCAGCCCGTTGAACCAGTTTTTCtt < 1:183165/130‑1 (MQ=255) ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCtt > 2:183165/1‑130 (MQ=255) ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACccgcc > 8:122587/1‑149 (MQ=255) gAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGGCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCGCGTTGAACCAGTTTTTCTTGACCATGGTGTAACccgcc < 6:258524/147‑1 (MQ=255) caGCCTGTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCgtgt > 2:30865/2‑149 (MQ=255) caGCCTGTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCgtgt > 1:268921/2‑149 (MQ=255) aGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGACGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTc > 3:222674/1‑149 (MQ=255) ctgTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTCGAt < 3:203429/147‑1 (MQ=255) | GATGTTCCGTGACGCGATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTCGAT > NC_002947/3330758‑3331047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |