Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,330,911 A→C 8.6% L364R (CTG→CGG)  nspC ← carboxynorspermidine/carboxyspermidine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,330,9110AC 8.6% 171.4 / 7.8 58L364R (CTG→CGG) nspCcarboxynorspermidine/carboxyspermidine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base C (3/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

ACGCGATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTCGATGAACGACAGG  >  NC_002947/3330769‑3331057
                                                                                                                                              |                                                                                                                                                  
aCGCGATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTg                                                                                                                                               >  2:171715/1‑148 (MQ=255)
 cgcgATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCTGTCGTAAGACCGGCtttc                                                                                                                                             <  4:140385/149‑4 (MQ=255)
 cgcgATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTgg                                                                                                                                              >  1:198749/1‑148 (MQ=255)
        gCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTa                                                                                                                                       <  6:257439/148‑1 (MQ=255)
          gATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTc                                                                                                                                    >  2:143073/1‑149 (MQ=255)
            tACGACCCAGTTCGTCGTTATGTTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCt                                                                                                                                   >  4:166563/1‑148 (MQ=255)
              cGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTc                                                                                                                                 >  2:265332/1‑148 (MQ=255)
                 cccAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGaaa                                                                                                                             <  1:265332/149‑1 (MQ=255)
                        cgtcgtTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACt                                                                                                                      >  7:163123/1‑149 (MQ=255)
                                     cgcgcACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACtt                                                                                                          <  1:171715/148‑1 (MQ=255)
                                     cgcgcACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTc                                                                                                         <  2:198749/149‑1 (MQ=255)
                                      gcgcACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTc                                                                                                         >  4:167838/1‑148 (MQ=255)
                                              gtgGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGcc                                                                                                >  7:243532/1‑149 (MQ=255)
                                                gggCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGt                                                                                              >  6:149836/1‑149 (MQ=255)
                                                 ggCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTc                                                                                             >  4:59467/1‑149 (MQ=255)
                                                  gCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAAATTACCCATTGCTCCATACTTGAAAGCCGGTCTTACGACAGGCTGGAAACCTAGACTTCGAAACCGAACTCCCGGACGAATTCGACGCAGCCGTc                                                                                             >  6:209408/1‑148 (MQ=255)
                                                   ccaccaCCTTGGCGACACCTCCTGCACCATTGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACTACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGa                                                                                           <  6:129849/149‑1 (MQ=255)
                                                    caccacCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGAc                                                                                                      >  6:235638/1‑137 (MQ=255)
                                                    caccacCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGAc                                                                                                      <  5:235638/137‑1 (MQ=255)
                                                    caccacCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAg                                                                                          >  7:134248/1‑149 (MQ=255)
                                                     accaccTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAgc                                                                                         <  8:166749/149‑1 (MQ=255)
                                                                gACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCAc                                                                              <  5:149836/149‑1 (MQ=255)
                                                                gACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCAc                                                                              >  4:67127/1‑149 (MQ=255)
                                                                  cacCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGa                                                                            <  8:163123/149‑1 (MQ=255)
                                                                   acCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGAt                                                                           <  8:209251/149‑1 (MQ=255)
                                                                    cctcctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATg                                                                          <  8:243532/149‑1 (MQ=255)
                                                                     ctcctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATg                                                                          >  2:316910/1‑148 (MQ=255)
                                                                        ctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGaa                                                                       <  3:167838/148‑1 (MQ=255)
                                                                        ctGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAg                                                                      >  3:252649/1‑149 (MQ=255)
                                                                           cACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGc                                                                    <  3:166563/148‑1 (MQ=255)
                                                                             ccAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGc                                                                    <  4:121311/146‑1 (MQ=255)
                                                                             ccAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGc                                                                    >  3:121311/1‑146 (MQ=255)
                                                                              cAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTGGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCt                                                                >  5:274970/1‑149 (MQ=255)
                                                                              cAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCt                                                                >  3:95357/1‑149 (MQ=255)
                                                                               aaTGATAAGAACGTTCTTCTTAAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACAGAACTCGCGGACGACTTCGACGCTGCCGTAGAGCTGCTTGAACACGATGGAAGGCATCtt                                                               >  1:181600/1‑149 (MQ=255)
                                                                                         aCGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGtt                                                      <  8:134248/148‑1 (MQ=255)
                                                                                         aCGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGtt                                                      <  5:209408/148‑1 (MQ=255)
                                                                                           gTTCTTCTTCAATTTACCCTTTGCTCCTTACTAGAAAGCCCGTCTTACGACAGGCAGGAAAAGTAGTCTTCGAAACCGAACTCGCGGAAGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGaa                                                   >  5:258524/1‑149 (MQ=255)
                                                                                                  ttcAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTAGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGttt                                            >  5:77894/1‑149 (MQ=255)
                                                                                                    cAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGtttt                                           <  6:77894/148‑1 (MQ=255)
                                                                                                     aaTTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTc                                         <  6:274970/149‑1 (MQ=255)
                                                                                                       tttACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCtt                                       <  3:59467/149‑1 (MQ=255)
                                                                                                       tttACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCtt                                       <  3:67127/149‑1 (MQ=255)
                                                                                                                     cTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTaa                          <  1:143073/148‑1 (MQ=255)
                                                                                                                          tttccaGCCTGTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACccgcc                    >  4:203429/6‑149 (MQ=255)
                                                                                                                          ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGaa                                                                       <  6:185700/98‑1 (MQ=255)
                                                                                                                          ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGaa                                                                       >  5:185700/1‑98 (MQ=255)
                                                                                                                          ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGTATCTTCAGCCCGTTGAACCAGTTTTTCtt                                       <  1:183165/130‑1 (MQ=255)
                                                                                                                          ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCtt                                       >  2:183165/1‑130 (MQ=255)
                                                                                                                          ttGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACccgcc                    >  8:122587/1‑149 (MQ=255)
                                                                                                                            gAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGGCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCGCGTTGAACCAGTTTTTCTTGACCATGGTGTAACccgcc                    <  6:258524/147‑1 (MQ=255)
                                                                                                                              caGCCTGTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCgtgt                >  2:30865/2‑149 (MQ=255)
                                                                                                                              caGCCTGTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCgtgt                >  1:268921/2‑149 (MQ=255)
                                                                                                                               aGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGACGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTc               >  3:222674/1‑149 (MQ=255)
                                                                                                                                  ctgTCGTAAGACCGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTCGAt            <  3:203429/147‑1 (MQ=255)
                                                                                                                                         aCGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGttt                                            <  3:236348/110‑1 (MQ=255)
                                                                                                                                         aCGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGttt                                            >  4:236348/1‑110 (MQ=255)
                                                                                                                                            tcAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATTGAATGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTCGATGAACGACAgg  <  3:263044/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ACGCGATGGCGATACGACCCAGTTCGTCGTTATGCTGCGCGCACTTGTGGGCCACCACCTTGGCGACACCTCCTGCACCAATGATAAGAACGTTCTTCTTCAATTTACCCTTTGCTCCTTACTTGAAAGCCGGTCTTACGACAGGCTGGAAACGTAGTCTTCGAAACCGAACTCGCGGACGACTTCGACGCTGCCGTCGAGCTGCTTGACCACGATGGAAGGCATCTTCAGCCCGTTGAACCAGTTTTTCTTGACCATGGTGTAACCCGCCGTGTCGATGAACGACAGG  >  NC_002947/3330769‑3331057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑