Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,380,540 T→A 8.3% L71Q (CTG→CAG)  tnpT‑I → cointegrate resolution protein T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,380,5400TA 8.3% 135.0 / 3.5 48L71Q (CTG→CAG) tnpT‑Icointegrate resolution protein T
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-01

AGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCA  >  NC_002947/3380396‑3380681
                                                                                                                                                |                                                                                                                                             
agaACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTc                                                                                                                                           <  8:69013/149‑1 (MQ=255)
     ccAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGcc                                                                                                                                       >  8:258381/1‑148 (MQ=255)
     ccAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGcc                                                                                                                                       >  4:271362/1‑148 (MQ=255)
     ccAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGcc                                                                                                                                       >  4:136045/1‑148 (MQ=255)
       aaGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAAc                                                                                                                                    <  7:289923/149‑1 (MQ=255)
       aaGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACACTTGGCCAACCTGGTCGGCCAAc                                                                                                                                    >  1:212441/1‑149 (MQ=255)
               aCGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGc                                                                                                                                        <  8:78190/137‑1 (MQ=255)
               aCGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGc                                                                                                                                        >  7:78190/1‑137 (MQ=255)
                       cGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTaga                                                                                                                    <  3:271362/149‑1 (MQ=255)
                          gTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAgagga                                                                                                                 <  2:212441/149‑1 (MQ=255)
                              aaCTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGAt                                                                                                             >  3:137024/1‑149 (MQ=255)
                              aaCTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGAt                                                                                                             >  1:288667/1‑149 (MQ=255)
                                      aaTACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGc                                                                                                     >  7:40376/1‑149 (MQ=255)
                                        tACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCa                                                                                                   <  7:258381/149‑1 (MQ=255)
                                          ccGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTgg                                                                                                                                             >  1:163503/1‑105 (MQ=255)
                                          ccGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTgg                                                                                                                                             <  2:163503/105‑1 (MQ=255)
                                            ggCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGt                                                                                               <  8:40376/149‑1 (MQ=255)
                                             gCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAgagga                                                                                                                 <  3:113291/130‑1 (MQ=255)
                                             gCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAgagga                                                                                                                 >  4:113291/1‑130 (MQ=255)
                                                cGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGcc                                                                                           >  8:110213/1‑149 (MQ=255)
                                                        accaTTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAgcg                                                                                   >  2:61893/1‑149 (MQ=255)
                                                        accaTTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAgcg                                                                                   <  5:55715/149‑1 (MQ=255)
                                                             tCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCg                                                                                                                                          >  4:64807/1‑89 (MQ=255)
                                                             tCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCg                                                                                                                                          <  3:64807/89‑1 (MQ=255)
                                                              cACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGaac                                                                              <  4:137024/148‑1 (MQ=255)
                                                               aCCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGaacaa                                                                            <  2:288667/149‑1 (MQ=255)
                                                                 cGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGaacaac                                                                           >  5:278335/1‑148 (MQ=255)
                                                                      ccTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACaa                                                                      <  1:61893/148‑1 (MQ=255)
                                                                               aCTCGAAGACGCCGAGCGCGGCCGAAATCCCGCCGCGAAACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACAGGCAGATCAGCTAGAGGCGGATGCGCAGGCCCCCCAGCCCCAGGAGCGCGCACACCTACACCGCGAAcccc                                                            >  7:134756/1‑146 (MQ=255)
                                                                                  cGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTa                                                                                                                      <  4:197016/88‑1 (MQ=255)
                                                                                  cGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTa                                                                                                                      >  3:197016/1‑88 (MQ=255)
                                                                                                gcgGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCaa                                           <  7:110213/149‑1 (MQ=255)
                                                                                                 cgGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAg                                          <  6:275691/149‑1 (MQ=255)
                                                                                                   gCCGAAATACCGCCGCGATACCGCTCAGCGTACAGTTGGCCAACCTGCTCGGCCTACTGGCAGATCCGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTCCCAGAACCAAGCt                                        <  8:134756/149‑1 (MQ=255)
                                                                                                       aaaTACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGCTCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGc                                    >  6:48845/1‑149 (MQ=255)
                                                                                                       aaaTACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGc                                    <  6:278335/149‑1 (MQ=255)
                                                                                                            ccgccgCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAAc                                                                     >  6:44887/1‑111 (MQ=255)
                                                                                                            ccgccgCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAAc                                                                     <  5:44887/111‑1 (MQ=255)
                                                                                                             cgccgcGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGcc                               >  6:162035/1‑148 (MQ=255)
                                                                                                                cgcgATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGaaa                           <  5:134959/149‑1 (MQ=255)
                                                                                                                       ccGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGaa                     <  3:136045/148‑1 (MQ=255)
                                                                                                                             aGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCcagca              <  5:146310/149‑1 (MQ=255)
                                                                                                                             aGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCcag                >  6:146310/1‑147 (MQ=255)
                                                                                                                                 aaCAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTg          >  8:132089/1‑149 (MQ=255)
                                                                                                                                  ccAGTTGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTg          >  5:73321/2‑148 (MQ=255)
                                                                                                                                      ttGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCcag                <  6:271270/138‑1 (MQ=255)
                                                                                                                                      ttGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCcag                >  5:271270/1‑138 (MQ=255)
                                                                                                                                           cAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCa  >  7:58506/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                             
AGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCA  >  NC_002947/3380396‑3380681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑