Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,442,394 G→A 9.7% E385K (GAG→AAG)  PP_3062 → tail length determination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,442,3940GA 9.7% 109.3 / 3.1 41E385K (GAG→AAG) PP_3062tail length determination protein
Reads supporting (aligned to +/- strand):  ref base G (18/19);  new base A (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

GAATACCGGCCTGCAGAAGGGCATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACTTAAGGCCGACTCCAAAAAG  >  NC_002947/3442255‑3442537
                                                                                                                                           |                                                                                                                                                 
gAATACCGGCCTGCAGAAGGGCATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGcc                                                                                                                                           >  1:36153/1‑148 (MQ=255)
 aaTACCGGCCTGCAGAAGGGCATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGcc                                                                                                                                           >  6:169049/1‑147 (MQ=255)
      cGGCCTGCAGAAGGGCATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGaa                                                                                                                                               <  1:108206/138‑1 (MQ=255)
      cGGCCTGCAGAAGGGCATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGaa                                                                                                                                               >  2:108206/1‑138 (MQ=255)
                  gggCATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAgcgc                                                                                                                        >  5:277043/1‑149 (MQ=255)
                     cATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTgg                                                                                                                     <  2:253777/149‑1 (MQ=255)
                      aTGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTg                                                                                                                      >  2:255370/1‑147 (MQ=255)
                                 tGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTgc                                                                                                         >  4:207770/1‑149 (MQ=255)
                                        tCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCgg                                                                                                  <  1:255370/149‑1 (MQ=255)
                                         ccATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGc                                                                                                 >  2:154372/1‑149 (MQ=255)
                                              gCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGata                                                                                             <  1:154372/148‑1 (MQ=255)
                                                      cATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGAAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGcc                                                                                     >  5:298265/1‑148 (MQ=255)
                                                      cATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGAAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGcc                                                                                     >  5:293624/1‑148 (MQ=255)
                                                          cGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCg                                                                                                <  3:234585/133‑1 (MQ=255)
                                                          cGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCg                                                                                                >  4:234585/1‑133 (MQ=255)
                                                                   gagaAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTc                                                                         >  7:296082/1‑147 (MQ=255)
                                                                     gaagaCCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTgc                                                                                                         >  3:119143/1‑113 (MQ=255)
                                                                     gaagaCCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTgc                                                                                                         <  4:119143/113‑1 (MQ=255)
                                                                                cGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACa                                                                                 >  8:78509/1‑126 (MQ=255)
                                                                                cGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACa                                                                                 <  7:78509/126‑1 (MQ=255)
                                                                                  gccaaggccaagCAGATCAAGGACGCCCAGGACAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGAGCTGGAGAAGAACCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGA‑‑GCTCACCGc                                                      >  1:131576/1‑149 (MQ=255)
                                                                                       ggccaagCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGAAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACg                                                 <  6:298265/149‑1 (MQ=255)
                                                                                       ggccaagCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGAAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACg                                                 <  6:293624/149‑1 (MQ=255)
                                                                                        gccaagCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACgg                                                <  6:187455/149‑1 (MQ=255)
                                                                                          caagCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTc                                                                                        >  3:87752/1‑109 (MQ=255)
                                                                                          caagCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTc                                                                                        <  4:87752/109‑1 (MQ=255)
                                                                                                    aaGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGACAGAACCggg                                     <  5:169049/148‑1 (MQ=255)
                                                                                                          gCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTa                              <  4:269769/149‑1 (MQ=255)
                                                                                                              aGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAg                          <  5:56638/149‑1 (MQ=255)
                                                                                                                      aTCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACtt                    >  3:74792/1‑147 (MQ=255)
                                                                                                                      aTCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACtt                    >  7:272991/1‑147 (MQ=255)
                                                                                                                         gACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACTTAAgg                <  3:207770/148‑1 (MQ=255)
                                                                                                                         gACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACTTAAgg                <  4:74792/148‑1 (MQ=255)
                                                                                                                          aCAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAgg                         <  6:232270/138‑1 (MQ=255)
                                                                                                                          aCAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAgg                         >  5:232270/1‑138 (MQ=255)
                                                                                                                              ggAAGTCGACCCGGAGAACGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGCTCAACGCGGG‑‑GATAACCGCCTACGGGCAGAACCCGGGCCTGTATGAGGAACATAAGGCCGACt          >  2:249103/1‑149 (MQ=255)
                                                                                                                                aaGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTGTGAGGAACTTAAGGCCGACTcc        <  2:36153/149‑1 (MQ=255)
                                                                                                                                     gACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACTTAAGGCCGACTCCaaaaa   <  2:312509/149‑1 (MQ=255)
                                                                                                                                     gACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACTTAAGGCCGACTCCaaaaa   <  4:299247/149‑1 (MQ=255)
                                                                                                                                     gACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACTTAAGGCCGACTCCaaa     >  3:299247/1‑147 (MQ=255)
                                                                                                                                        ccGGTGAAGGCCAATGCGGCACTGGAAGCGCTGGAGAAGAACCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGCCAGCTCACCGCCTACGTGCAGAACCGGGGCCTGTATGAGGAACTTAAGGCCGACTCCAAAAAg  <  1:249103/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
GAATACCGGCCTGCAGAAGGGCATGAACGTCATTGAGGCGTCCATGGCCCTGGCCATGCGTTACGTCGAGAAGACCGACCCGGCCAAGGCCAAGCAGATCAAGGACGCCCAGGCCAAGATCGACAAGGAAGTCGACCCGGAGAAGGCCAAGGCGGCACTGGAAGCGCTGGAGAAGACCCTGCGCACCGGCGATATCTTCGCCGACATGCAGGTCAAGGCGGC‑‑GCTCACCGCCTACGGGCAGAACCGGGGCCTGTATGAGGAACTTAAGGCCGACTCCAAAAAG  >  NC_002947/3442255‑3442537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑