Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,526,480 A→G 8.9% V106V (GTA→GTG)  PP_3117 → DNA lesion error‑prone processing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,526,4800AG 8.9% 121.9 / 2.9 45V106V (GTA→GTG) PP_3117DNA lesion error‑prone processing protein
Reads supporting (aligned to +/- strand):  ref base A (21/20);  new base G (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

CCAACCCCGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTGCGGCGCTTGCAG  >  NC_002947/3526332‑3526603
                                                                                                                                                    |                                                                                                                           
ccAACCCCGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTa                                                                                                                             <  4:306642/149‑1 (MQ=255)
  aaCCCCGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTa                                                                                                                             >  3:56162/1‑147 (MQ=255)
   aCCCCGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCAACGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAg                                                                                                                          >  5:248739/1‑149 (MQ=255)
     cccGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGt                                                                                                                        >  2:279927/1‑149 (MQ=255)
     cccGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGt                                                                                                                        <  6:158838/149‑1 (MQ=255)
      ccGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGATGGTGGATCCACCCTTCCTCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTg                                                                                                                       >  7:267373/1‑149 (MQ=255)
       cGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTg                                                                                                                       >  4:162765/1‑148 (MQ=255)
          ttGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTGCAGGTGGATg                                                                                                                   >  7:4976/1‑149 (MQ=255)
          ttGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTGCAGGTGGATg                                                                                                                   >  7:444/1‑149 (MQ=255)
                     tGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGcc                                                                                                        >  7:159528/1‑149 (MQ=255)
                       cAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTGCAGGTGGATGCCGAGCCTGcc                                                                                                        <  8:4976/147‑1 (MQ=255)
                       cAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTGCAGGTGGATGCCGAGCCTGcc                                                                                                        <  8:444/147‑1 (MQ=255)
                        aGTTGCCGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGAt                                                                                                     <  6:290767/149‑1 (MQ=255)
                             cTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCt                                                                                                <  4:56162/149‑1 (MQ=255)
                               gtgGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCtgt                                                                                              >  5:295141/1‑149 (MQ=255)
                                  gCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCCCCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCGCTGTggg                                                                                           >  3:27371/1‑149 (MQ=255)
                                  gCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTggg                                                                                           >  6:135630/1‑149 (MQ=255)
                                   ccAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGc                                                                                          >  3:8399/1‑149 (MQ=255)
                                     aaCCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTg                                                                                        >  7:276430/1‑149 (MQ=255)
                                     aaCCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTg                                                                                        >  5:38961/1‑149 (MQ=255)
                                      aCCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGc                                                                                       <  5:223709/149‑1 (MQ=255)
                                       cccTGGGTCGTCTGAGTGCAGAAGCCAGCAGAGTGTTGCTGGTGGCTCCACCCTTCATCGCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGTACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGcc                                                                                      <  8:159528/149‑1 (MQ=255)
                                              tCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGt                                                                               >  5:19518/1‑149 (MQ=255)
                                                ggcggcgTGCAGAAGCCAGCAGAGGGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCCGGCAGCGGGGGGGGACCTGCGCTGGCTGGTACAGGGGGATGCCGAGCCTGCCGATGCCCTGGGGGCTGCCGAACAGTGc                                                                             <  4:235104/143‑1 (MQ=255)
                                                ggCTGAGTGCAGCAGCCATCAGCGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTGGCACCGGCCTGGCCGGCAGCGGGGGTGGACCTGCGCTTGCGGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGc                                                                             <  4:231018/149‑1 (MQ=255)
                                                                cagcagAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGtt                                                             >  7:106841/1‑149 (MQ=255)
                                                                 agcagAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTg                                                            >  4:89870/1‑149 (MQ=255)
                                                                 agcagAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTg                                                            >  6:203608/1‑149 (MQ=255)
                                                                        tggtgCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCgcggcg                                                     <  8:276430/149‑1 (MQ=255)
                                                                        tggtgCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCgcggcg                                                     <  6:38961/149‑1 (MQ=255)
                                                                                gtggCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGgtgctgtg                                             <  6:19518/149‑1 (MQ=255)
                                                                                gtggCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGgtgctgtg                                             <  3:162765/149‑1 (MQ=255)
                                                                                  ggCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGgtgctgtgct                                           <  3:205959/149‑1 (MQ=255)
                                                                                     tCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGc                                        >  4:188560/1‑149 (MQ=255)
                                                                                     tCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGc                                        >  4:189060/1‑149 (MQ=255)
                                                                                       cACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGcc                                       <  1:279927/148‑1 (MQ=255)
                                                                                       cACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCg                                      <  6:295141/149‑1 (MQ=255)
                                                                                                tCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGcc                             >  4:206062/1‑149 (MQ=255)
                                                                                                 cccTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGaa                                                                                   >  5:148764/1‑94 (MQ=255)
                                                                                                   cTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTACTGTGCTGGCCGGAACGTGCCGAt                          >  3:269859/1‑149 (MQ=255)
                                                                                                        gCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACcg                     >  8:188163/1‑149 (MQ=255)
                                                                                                                    caggcagCGGGGGTGGACGTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTgcggcg         <  6:248739/149‑1 (MQ=255)
                                                                                                                         agCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTACTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTGCGGCGCTTGc    <  4:269859/149‑1 (MQ=255)
                                                                                                                          gCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTGCGGCGCTTGCa   <  5:135630/149‑1 (MQ=255)
                                                                                                                           cGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTGCGGCGCTTGCAg  <  5:203608/149‑1 (MQ=255)
                                                                                                                           cGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTGCGGCGCTTGCAg  <  8:106841/149‑1 (MQ=255)
                                                                                                                           cGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTGCGGCGCTTGCAg  <  4:8399/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                           
CCAACCCCGGTTGTGGCGAACTGCAGTTGCTGTGGCCAACCCTGGCTCGGCTGAGTGCAGAAGCCAGCAGAGTGGTGCTGGTGGCTCCACCCTTCATCCCTTTTGCACCGGCCTGGCAGGCAGCGGGGGTGGACCTGCGCTGGCTGGTACAGGTGGATGCCGAGCCTGCCGATGCCCTGTGGGCTGCCGAACAGTGCCTGCGCTCGGGCAGTTGCGCGGCGGTGCTGTGCTGGCCGGAACGTGCCGATGACCGCGCCCTGCGGCGCTTGCAG  >  NC_002947/3526332‑3526603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑