| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,542,318 | A→G | 6.8% | Y135Y (TAT→TAC) | galE ← | UDP‑glucose 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,542,318 | 0 | A | G | 6.8% | 174.0 / 2.5 | 60 | Y135Y (TAT→TAC) | galE | UDP‑glucose 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (22/33); new base G (2/2); total (24/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
ATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTG > NC_002947/3542174‑3542465 | aTGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACg < 8:51221/149‑1 (MQ=255) gTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGtt < 7:74125/149‑1 (MQ=255) gCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGc < 4:265576/149‑1 (MQ=255) gTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCt > 6:121362/1‑149 (MQ=255) tGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCtt > 7:184818/1‑149 (MQ=255) ccTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagc < 3:170276/132‑3 (MQ=255) ccTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagc > 4:170276/1‑130 (MQ=255) gCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCa < 2:301623/149‑1 (MQ=255) cTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATACCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCat < 8:222138/149‑1 (MQ=255) ggCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatc < 3:241696/148‑1 (MQ=255) ggCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatc > 4:241696/1‑148 (MQ=255) gCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatc < 5:121362/147‑1 (MQ=255) ttCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagcaaa < 8:7285/124‑6 (MQ=255) ttCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagcaaa > 7:7285/1‑119 (MQ=255) gTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGt < 5:177234/149‑1 (MQ=255) ccGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCggg < 3:253553/149‑1 (MQ=255) aTTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTg > 1:171717/1‑138 (MQ=255) aTTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTg < 2:171717/138‑1 (MQ=255) aaaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgt > 2:299439/1‑109 (MQ=255) aaaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgt < 1:299439/109‑1 (MQ=255) aaaTAGCGCAGGCTTACCGACTTCAAACCAAAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAc < 4:201749/149‑1 (MQ=255) aaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcg > 1:228106/1‑149 (MQ=255) aaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcg < 2:292521/149‑1 (MQ=255) tAGCGCAGGCTTACCGACTTCAAACCATAGCCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCGTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggc < 2:148463/149‑1 (MQ=255) ggCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTc > 7:65580/1‑149 (MQ=255) aCCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaag < 8:65580/148‑1 (MQ=255) aCCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaa < 6:251958/147‑1 (MQ=255) gACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCt < 8:293062/96‑1 (MQ=255) cAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAacac < 2:228106/147‑1 (MQ=255) aTAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGcc < 6:44824/149‑1 (MQ=255) gTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑cccg > 2:202735/1‑147 (MQ=255) gTCGAAGTCCACAAGAATCTGCTCGACCATCCAATTCCTCAACCCATACGCCTAGATCGGCCCCATGGTGTGTGCTTCAACGATCGGCACGTACTGCGCGTTGCCCTATACGCCGGCCCACGAAGAAAACACCAGGTGCCTCATG‑CCgg > 2:207312/1‑149 (MQ=255) gTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGa > 2:191668/1‑149 (MQ=255) cTCAAGAATCTGCTCGACCATCAATTTGCTAACCCCATACGGGTTTATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGCAGAAAACACAAGGTGCCTGATG‑CCGGCAGTGAcaa > 8:143935/1‑147 (MQ=255) tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcgg > 8:54847/1‑106 (MQ=255) tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcgg < 7:54847/106‑1 (MQ=255) cAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCAt < 5:259960/141‑1 (MQ=255) cAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCAt > 6:259960/1‑141 (MQ=255) aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCGCTTGGTGTGTGCTTCATCGATCGGCACGTACTGGGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑c < 5:203642/134‑1 (MQ=255) aaGAATCTGCTCGACAATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTAATCGATCGGCACGTACTGCGGGTTGCCGTATAAGGCGGCACTCGAAGAAAACAACAGGTGCCTGAtcc > 6:203642/1‑132 (MQ=255) aGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCga < 5:103242/110‑1 (MQ=255) aGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCga > 6:103242/1‑110 (MQ=255) gAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGcc > 8:194980/1‑149 (MQ=255) gCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGa > 4:140319/1‑135 (MQ=255) gCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGa < 3:140319/135‑1 (MQ=255) cTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTgcagca < 1:263125/149‑1 (MQ=255) gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcgg < 2:245867/89‑1 (MQ=255) gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcgg > 1:245867/1‑89 (MQ=255) gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTgcagca < 7:194980/146‑1 (MQ=255) catTTGCTCAACCCTTTGGGGTTGATGGGGCGCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCGGTATACGGCGGCACTCGAAGAAATCACCAGGTGCCTGATGACCGGCATTGACCATGGCCTGCAGGAGGGAAAggg < 1:207312/149‑1 (MQ=255) catTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGACCGGCATTGACCATGGCCTGCAGCAGGGAAAggg < 1:202735/149‑1 (MQ=255) catTTGCTCAACCCATAAGGGTTGATCGGCCCCTTGGGGTGTGCTTCATCGATCGGCACGTACTGCGTGTTGCAGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGCCCATGGCCTGCAGCAGGGAAAggg < 7:143935/148‑1 (MQ=255) atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAggg > 1:187153/1‑147 (MQ=255) atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAggg < 2:187153/147‑1 (MQ=255) atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg < 1:191668/149‑1 (MQ=255) cTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg > 4:89734/1‑144 (MQ=255) cTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg < 3:89734/144‑1 (MQ=255) aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACAcc < 2:166291/93‑1 (MQ=255) aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACAcc > 1:166291/1‑93 (MQ=255) cATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACgttg > 4:226671/1‑149 (MQ=255) | ATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATG‑CCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTG > NC_002947/3542174‑3542465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |