Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,553,793 C→T 6.4% R154C (CGC→TGC)  PP_3138 → VirK domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,553,7930CT 6.4% 186.9 / 2.3 62R154C (CGC→TGC) PP_3138VirK domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (35/23);  new base T (2/2);  total (37/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

ACCTGAGGCTGTGTGACCTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAA  >  NC_002947/3553647‑3553941
                                                                                                                                                  |                                                                                                                                                     
aCCTGAGGCTGTGTGACCTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCg                                                                                                                                                      >  3:113092/1‑148 (MQ=255)
  cTGAGGCTGTGTGACCTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTCGTGCTGCACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAg                                                                                                                                                   >  4:302302/1‑149 (MQ=255)
       gCTGTGTGACCTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAgg                                                                                                                                             >  2:144972/1‑149 (MQ=255)
             tgACCTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGaaa                                                                                                                                       <  4:137796/149‑1 (MQ=255)
             tgACCTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCT‑GGCTGCGGCCCAGGGTGaa                                                                                                                                        <  8:130574/148‑1 (MQ=255)
                ccTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAAcct                                                                                                                                    <  4:113092/149‑1 (MQ=255)
                 cTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAgg                                                                                                                                             <  1:254133/139‑1 (MQ=255)
                 cTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAgg                                                                                                                                             >  2:254133/1‑139 (MQ=255)
                  tGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAAcctgc                                                                                                                                  >  1:265446/1‑149 (MQ=255)
                      cAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGt                                                                                                                              >  4:95956/1‑149 (MQ=255)
                         ccACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCa                                                                                                                           >  8:213021/1‑149 (MQ=255)
                           aCTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCAtt                                                                                                                         >  1:247542/1‑149 (MQ=255)
                                    gCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCT‑GGCTGCGGCCCAGGGTGaa                                                                                                                                        <  8:79513/125‑1 (MQ=255)
                                    gCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCT‑GGCTGCGGCCCAGGGTGaa                                                                                                                                        >  7:79513/1‑125 (MQ=255)
                                        ttGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAgg                                                                                                            >  7:280482/1‑149 (MQ=255)
                                            cTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCAt                                                                                                        >  5:211299/1‑149 (MQ=255)
                                             tGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATc                                                                                                       >  6:231255/1‑149 (MQ=255)
                                               gTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCca                                                                                                     <  3:95956/149‑1 (MQ=255)
                                                     gACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTggtgcggt                                                                                                                 <  5:33403/131‑1 (MQ=255)
                                                     gACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTggtgcggt                                                                                                                 >  6:33403/1‑131 (MQ=255)
                                                     gACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAg                                                                                                <  7:213021/148‑1 (MQ=255)
                                                          gCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTggtgcggt                                                                                                                 <  5:66280/126‑1 (MQ=255)
                                                          gCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTggtgcggt                                                                                                                 >  6:66280/1‑126 (MQ=255)
                                                          gCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATc                                                                                                       >  3:226472/1‑136 (MQ=255)
                                                          gCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATc                                                                                           >  7:161275/1‑148 (MQ=255)
                                                               tCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTc                                                                                                                            <  7:101102/110‑1 (MQ=255)
                                                               tCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTc                                                                                                                            >  8:101102/1‑110 (MQ=255)
                                                               tCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGc                                                                                     >  2:250210/1‑149 (MQ=255)
                                                                     tCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAggg                                                                                                           <  2:148880/121‑1 (MQ=255)
                                                                      cAAGCGCGAAGGCGAGCTGGTGCTGAACCAGTTCCAGTCCGACCTGCGTGTGGCATACCTTGCCTTCACCATGGGCCGCAG‑CCAGGGTGAAACCTGCCTGATCATTGGTGCGGTGCACGGCATCAAAAAAGGTATCCACAGCTAAACCt                                                                              >  1:243212/1‑149 (MQ=255)
                                                                       aaGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTc                                                                             >  1:162565/1‑149 (MQ=255)
                                                                        aGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGtt                                                                                                                             >  1:286748/1‑100 (MQ=255)
                                                                        aGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGtt                                                                                                                             <  2:286748/100‑1 (MQ=255)
                                                                        aGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCg                                                                            >  4:32066/1‑149 (MQ=255)
                                                                         gcgcGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCATGGCATCCACAAAGGTATCGACAGCGAAACCTCGc                                                                           >  6:121216/1‑149 (MQ=255)
                                                                           gcgAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTg                                                                         <  1:144972/149‑1 (MQ=255)
                                                                               aGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCa                                                                     >  1:133178/1‑149 (MQ=255)
                                                                                    aGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTAc                                                                <  2:247542/149‑1 (MQ=255)
                                                                                         gTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtga                                                           <  7:21609/149‑1 (MQ=255)
                                                                                         gTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtga                                                           >  5:270137/1‑149 (MQ=255)
                                                                                             tGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacct                                                        >  2:134678/1‑148 (MQ=255)
                                                                                               aaCCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCATGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacctga                                                      <  5:121216/148‑1 (MQ=255)
                                                                                               aaCCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacctgac                                                     >  2:101020/1‑149 (MQ=255)
                                                                                                  cTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacatgacc                                                    >  3:230599/1‑147 (MQ=255)
                                                                                                     ttCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGATCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAg                                                 <  1:101020/147‑1 (MQ=255)
                                                                                                        cAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACtt                                            >  7:258358/1‑149 (MQ=255)
                                                                                                                 cTGCGTGTGGCATCCCTTGCCTTCTCCCTGGGCCGCAG‑ACAGGGTTAAACCAGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCTAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGc                                    <  3:302302/148‑1 (MQ=255)
                                                                                                                 cTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCt                                   >  7:261266/1‑149 (MQ=255)
                                                                                                                            aTCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACg                          >  6:213590/1‑147 (MQ=255)
                                                                                                                            aTCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACg                          >  3:182045/1‑147 (MQ=255)
                                                                                                                              cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                       >  5:206715/1‑132 (MQ=255)
                                                                                                                              cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                       <  6:206715/132‑1 (MQ=255)
                                                                                                                              cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                       <  6:202023/132‑1 (MQ=255)
                                                                                                                              cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                       >  5:202023/1‑132 (MQ=255)
                                                                                                                                gcaggtttCACCCT‑GGCTGCGGCCCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCt                    >  7:65166/7‑149 (MQ=255)
                                                                                                                                cTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCt                    <  4:182045/149‑1 (MQ=255)
                                                                                                                                   gCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTg                   <  8:280482/147‑1 (MQ=255)
                                                                                                                                     cTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGc               >  2:278827/1‑149 (MQ=255)
                                                                                                                                      ttCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGc                      >  4:215023/1‑141 (MQ=255)
                                                                                                                                      ttCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGc                      <  3:215023/141‑1 (MQ=255)
                                                                                                                                        cACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTc             <  2:265446/148‑1 (MQ=255)
                                                                                                                                               ggCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGt      <  8:261266/148‑1 (MQ=255)
                                                                                                                                                  cGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGaa  <  3:32066/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ACCTGAGGCTGTGTGACCTGTCCAGCCACTCGCCGGGCTGTTGCCTGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAA  >  NC_002947/3553647‑3553941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑